当前位置： SCI文献检索 > CHEMICO-BIOLOGICAL INTERACTIONS期刊下所有文献 > Anti-proliferative and pro-apoptotic effect of CPT13, a novel camptothecin analog, on human colon cancer HCT8 cell line.

Anti-proliferative and pro-apoptotic effect of CPT13, a novel camptothecin analog, on human colon cancer HCT8 cell line.

Abstract：

:10-(2-pyrazolyl-ethoxy)-(20S)-camptothecin (CPT13) is a novel semi-synthetic analogue of camptothecin, our previous report had shown that it possessed higher in vitro cytoxicity activity towards human colon cancer HCT8 cell line than topotecan. In this study, the anti-proliferative effect of CPT13 on HCT8 cell line in vitro was analyzed. In order to further explore the underlying mechanism of cell growth inhibition of CPT13 towards HCT8 cell line, the cell cycle distribution, apoptosis proportion, the nuclei morphological changes and caspase-8 and caspase-3 activities were measured. Additionally the changes of mitochondrial morphology and membrane potential (DeltaPsim) were analyzed by atomic force microscopy (AFM) and flow cytometry, respectively. The results showed that CPT13 inhibited HCT8 cell growth by causing cell cycle arrest at G2/M transition and induced apoptosis, as evidenced by the typical apoptotic morphology such as condensation and fragmentation of nuclei and formation of apoptotic bodies. The changes of mitochondrial morphology, dose-dependently decrease in DeltaPsim and the enhancement of caspase-8 and caspase-3 activities were observed in different concentrations of drug treatment group. Our results suggest that CPT13 induces apoptosis by alternations of mitochondrial transmembrane depolarization, activation of caspase-8 and caspase-3. Therefore, CPT13 appears to be a potent drug against human colon cancer via induction of apoptosis and may be used as an alternative drug to therapy cancer.

journal_name

Chem Biol Interact

journal_title

Chemico-biological interactions

authors

Wang LM,Li QY,Zu YG,Fu YJ,Chen LY,Lv HY,Yao LP,Jiang SG

doi

10.1016/j.cbi.2008.07.005

subject

Has Abstract

pub_date

2008-11-25 00:00:00

pages

165-72

issue

2-3

eissn

0009-2797

issn

1872-7786

pii

S0009-2797(08)00370-0

journal_volume

176

pub_type

杂志文章

相关文献

CHEMICO-BIOLOGICAL INTERACTIONS文献大全
  • Suppressive effects of pelargonidin on lipopolysaccharide-induced inflammatory responses.

    abstract：:Pelargonidin (PEL) is a well-known red pigment found in plants, and has been reported as having important biological activities that are potentially beneficial for human health. Here, we tested the possible use of PEL in the treatment of lipopolysaccharide (LPS)-mediated vascular inflammatory responses. The anti-infla...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2019.02.007

    authors： Lee BS,Lee C,Yang S,Park EK,Ku SK,Bae JS

    更新日期：2019-04-01 00:00:00

  • The inhibition of lipid peroxidation by disulfiram prevents the killing of cultured hepatocytes by allyl alcohol, tert-butyl hydroperoxide, hydrogen peroxide and diethyl maleate.

    abstract：:Disulfiram is a potent antioxidant that prevented the peroxidation of microsomal phospholipids induced by ADP/Fe3+ at concentrations as low as 1 microM. However, disulfiram had a biphasic action when used to assess the role of lipid peroxidation in the killing of cultured hepatocytes by an acute oxidative stress. At a...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/0009-2797(89)90003-3

    authors： Kyle ME,Serroni A,Farber JL

    更新日期：1989-01-01 00:00:00

  • 20(S)-protopanaxadiol (PPD) alleviates scopolamine-induced memory impairment via regulation of cholinergic and antioxidant systems, and expression of Egr-1, c-Fos and c-Jun in mice.

    abstract：:20(S)-protopanaxadiol (PPD) possesses various biological properties, including anti-inflammatory, antitumor and anti-fatigue properties. Recent studies found that PPD functioned as a neurotrophic agent to ameliorate the sensory deficit caused by glutamate-induced excitotoxicity through its antioxidant effects and exhi...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2017.11.008

    authors： Lu C,Dong L,Lv J,Wang Y,Fan B,Wang F,Liu X

    更新日期：2018-01-05 00:00:00

  • Achieving resistance specificity in prostate cancer.

    abstract：:Prostate (CaP) cancer is the second-leading cause of cancer-related mortality in men in Western societies. Androgen receptor (AR) signaling is a critical survival pathway for prostate cancer cells, and androgen-deprivation therapy (ADT) remains the principal treatment for patients with locally advanced and metastatic ...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章,评审

    doi：10.1016/j.cbi.2016.10.002

    authors： Wadhwa B,Dumbre R

    更新日期：2016-12-25 00:00:00

  • Medium-chain fatty acids and glutathione derivatives as inhibitors of S-nitrosoglutathione reduction mediated by alcohol dehydrogenase 3.

    abstract：:Alcohol dehydrogenase 3 (ADH3) has emerged as an important regulator of protein S-nitrosation in its function as S-nitrosoglutathione (GSNO) reductase. GSNO depletion is associated with various disease conditions, emphasizing the potential value of a specific ADH3 inhibitor. The present study investigated inhibition o...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2009.01.008

    authors： Staab CA,Hellgren M,Grafström RC,Höög JO

    更新日期：2009-06-15 00:00:00

  • Impact of exogenous lysolipids on sensitive and multidrug resistant K562 cells: 1H NMR studies.

    abstract：:The ability of lysolipids to enter into a membrane bi-layer and disturb the membrane structure was used to study the behavior of K562 erythroleukemic cells, K562 wild type (K562wt) as well as the multidrug resistant cells K562adr. Both types of cells, when analyzed by proton NMR spectroscopy exhibit the high resolutio...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2004.12.005

    authors： Traïkia M,Marbeuf-Gueye C,Hantz E,Le Moyec L

    更新日期：2005-01-15 00:00:00

  • Comparison of pharmacokinetic and pharmacodynamic profiles of aspirin following oral gavage and diet dosing in rats.

    abstract：:Aspirin is one of the oldest drugs and has been purported to have multiple beneficial effects, including prevention of cardiovascular disease and cancer, in addition to its original indication for treatment of inflammation, fever and pain. In cancer chemoprevention studies using animal models, two methods of aspirin a...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2008.10.008

    authors： Kapetanovic IM,Bauer KS,Tessier DM,Lindeblad MO,Zakharov AD,Lubet R,Lyubimov A

    更新日期：2009-05-15 00:00:00

  • Compounds with the dioxopyrimidine cycle inhibit cholinesterases from different groups of animals.

    abstract：:We firstly synthesized derivatives of 6-methyluracil, alloxazine, and xanthine, containing omega-tetraalkylammonium (TAA) groups at the N(1) and N(3) atoms in a pyrimidine cycle and assayed their anticholinesterase activities. Compounds with triethylpentylammoniumalkyl groups behaved as typical reversible inhibitors o...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2008.05.037

    authors： Anikienko KA,Bychikhin EA,Reznik VS,Akamsin D,Galyametdinova IV

    更新日期：2008-09-25 00:00:00

  • Non-enzymatic modifications of prostaglandin H synthase 1 affect bifunctional enzyme activity - Implications for the sensitivity of blood platelets to acetylsalicylic acid.

    abstract：:Due to its ability to inhibit the blood platelet PGHS-1, acetylsalicylic acid (ASA, Aspirin(®)) is widely used as a preventive agent in atherothrombotic diseases. However, its beneficial effects seem to be lower in diabetic patients, suggesting that protein glycation may impair effective ASA-mediated acetylation proce...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2016.04.021

    authors： Kassassir H,Siewiera K,Talar M,Stec-Martyna E,Pawlowska Z,Watala C

    更新日期：2016-06-25 00:00:00

  • Camel milk attenuates the biochemical and morphological features of diabetic nephropathy: inhibition of Smad1 and collagen type IV synthesis.

    abstract：:Diabetic nephropathy (DN) is a common microvascular complication of diabetes mellitus (DM) that worsens its morbidity and mortality. There is evidence that camel milk (CM) improves the glycemic control in DM but its effect on the renal complications especially the DN remains unclear. Thus the current study aimed to ch...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2015.01.013

    authors： Korish AA,Abdel Gader AG,Korashy HM,Al-Drees AM,Alhaider AA,Arafah MM

    更新日期：2015-03-05 00:00:00

  • Multifunctional memantine nitrate significantly protects against glutamate-induced excitotoxicity via inhibiting calcium influx and attenuating PI3K/Akt/GSK3beta pathway.

    abstract：:Overactivation of N-methyl-D-aspartate (NMDA) receptors has been associated with neurodegenerative disorders such as Alzheimer's disease (AD), cerebral vascular disorders and amyotrophic lateral sclerosis (ALS). We have previously designed and synthesized a series of memantine nitrate and some of them have shown vesse...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2020.109020

    authors： Liu Z,Qiu X,Mak S,Guo B,Hu S,Wang J,Luo F,Xu D,Sun Y,Zhang G,Cui G,Wang Y,Zhang Z,Han Y

    更新日期：2020-07-01 00:00:00

  • 6-Shogaol enhances renal carcinoma Caki cells to TRAIL-induced apoptosis through reactive oxygen species-mediated cytochrome c release and down-regulation of c-FLIP(L) expression.

    abstract：:6-Shogaol, a potent bioactive compound in ginger (Zingiber officinale Roscoe), has been reported for anti-inflammatory and anti-cancer activity. In this study, we investigated the effect of 6-shogaol to enhance tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis. The combined treatment w...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2015.01.020

    authors： Han MA,Woo SM,Min KJ,Kim S,Park JW,Kim DE,Kim SH,Choi YH,Kwon TK

    更新日期：2015-02-25 00:00:00

  • Biochemical and biological evaluation of novel potent coumarin inhibitor of 17β-HSD type 1.

    abstract：:Human 17β-hydroxysteroid dehydrogenase (17β-HSD) type 1 is an enzyme that acts at the pre-receptor level. It catalyzes the NADPH-dependent reduction of the weak estrogen estrone into the most potent estrogen 17β-estradiol, which exerts proliferative effects via estrogen receptors. Overexpression of 17β-HSD type 1 in e...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2011.01.002

    authors： Starčević S,Kocbek P,Hribar G,Rižner TL,Gobec S

    更新日期：2011-05-30 00:00:00

  • Resveratrol mitigate structural changes and hepatic stellate cell activation in N'-nitrosodimethylamine-induced liver fibrosis via restraining oxidative damage.

    abstract：:Resveratrol, a polyphenol, found in skin of red grapes, peanuts and berries possesses anti-inflammatory, anti-carcinogenic and lipid modulation properties. Here, we demonstrate in vivo antifibrotic activity of resveratrol in a mammalian model, wherein hepatic fibrosis was induced by N'-nitrosodimethylamine (NDMA) admi...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2014.07.007

    authors： Ahmad A,Ahmad R

    更新日期：2014-09-25 00:00:00

  • Joint toxic effects of the type-2 alkene electrophiles.

    abstract：:Human populations are exposed to complex environmental mixtures of acrolein, methylvinyl ketone (MVK) and other type-2 alkenes. Many members of this chemical class are electrophiles that possess a common molecular mechanism of toxicity; i.e., protein inactivation via formation of stable cysteine adducts. Therefore, ac...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2016.06.014

    authors： Zhang L,Geohagen BC,Gavin T,LoPachin RM

    更新日期：2016-07-25 00:00:00

  • Adverse hepatic drug reactions: inflammatory episodes as consequence and contributor.

    abstract：:Susceptibility to drug toxicity is influenced by a variety of factors, both genetic and environmental. The focus of this article is the evidence addressing the hypothesis that inflammation is both a result of and a susceptibility factor for drug toxicity, with an emphasis on liver as a target organ. Results of studies...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章,评审

    doi：10.1016/j.cbi.2004.09.002

    authors： Ganey PE,Luyendyk JP,Maddox JF,Roth RA

    更新日期：2004-11-01 00:00:00

  • Hispidulin inhibits adipogenesis in 3T3-L1 adipocytes through PPARγ pathway.

    abstract：:Hispidulin, a natural flavone, has been reported to have diverse pharmacological effects, including antifungal, antioxidant, and antithrombotic properties. However, an anti-adipogenic effect has not yet been reported, which is the focus of the current study. Hispidulin suppressed the differentiation of adipocytes and ...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2018.07.027

    authors： Lee SG,Kim JS,Min K,Kwon TK,Nam JO

    更新日期：2018-09-25 00:00:00

  • Oxidant-increased proteolysis in rat liver slices: effect of bromotrichloromethane, antioxidants and effectors of proteolysis.

    abstract：:Proteolysis and lipid peroxidation were evaluated in rat liver slices incubated in the presence of the oxidant bromotrichloromethane and effectors of proteolysis. Proteolysis was evaluated by S-amino acids and lipid peroxidation by thiobarbituric acid-reactive substances (TBARS) released into the incubation medium. Th...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/0009-2797(90)90097-7

    authors： Zamora R,Hidalgo FJ,Tappel AL

    更新日期：1990-01-01 00:00:00

  • Mechanism of action and fate of the fungicide chlorothalonil (2,4,5,6-tetrachloroisophthalonitrile) in biological systems. 2. In vitro reactions.

    abstract：:The reaction characteristics of chlorothalonil with glyceraldehyde-3-phosphate dehydrogenase (GPDH), from yeast, (EC 1.2.1.12) were studied in vitro. Enzyme inhibition was related to the amount of [14C]chlorothalonil bound to the protein. Kinetics of enzyme inhibition was non-competitive for the substrate glyceraldehy...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/0009-2797(75)90069-1

    authors： Long JW,Siegel MR

    更新日期：1975-06-01 00:00:00

  • Immune up regulatory response of a non-caloric natural sweetener, stevioside.

    abstract：:Immunomodulation is a process, which alters the immune system of an organism by interfering with its functions. This interference results in either immunostimulation or immunosuppression. An immunomodulator is any substance that helps to regulate the immune system. This "regulation" is a normalization process, so that...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2008.01.008

    authors： Sehar I,Kaul A,Bani S,Pal HC,Saxena AK

    更新日期：2008-05-28 00:00:00

  • Human and rodent aldo-keto reductases from the AKR1B subfamily and their specificity with retinaldehyde.

    abstract：:NADP(H)-dependent cytosolic aldo-keto reductases (AKR) are mostly monomeric enzymes which fold into a typical (α/β)(8)-barrel structure. Substrate specificity and inhibitor selectivity are determined by interaction with residues located in three highly variable loops (A, B, and C). Based on sequence identity, AKR have...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2011.02.007

    authors： Ruiz FX,Moro A,Gallego O,Ardèvol A,Rovira C,Petrash JM,Parés X,Farrés J

    更新日期：2011-05-30 00:00:00

  • Bis(12)-hupyridone, a novel acetylcholinesterase inhibitor, protects against glutamate-induced neuronal excitotoxicity via activating α7 nicotinic acetylcholine receptor/phosphoinositide 3-kinase/Akt cascade.

    abstract：:Bis(12)-hupyridone (B12H), derived from the Chinese medicinal component huperzine A, was originally designed as a novel acetylcholinesterase (AChE) inhibitor. In this paper, we report that B12H (24-h pretreatment) effectively blocked glutamate-induced neuronal excitotoxicity in cerebellar granule neurons (CGNs). Howev...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2012.10.003

    authors： Cui W,Hu S,Chan HH,Luo J,Li W,Mak S,Choi TC,Rong J,Carlier PR,Han Y

    更新日期：2013-03-25 00:00:00

  • Time-resolved fluorescence spectroscopy of hematoporphyrin-derivative in human lymphocytes.

    abstract：:This paper reports on time-resolved microfluorimetric measurements on hematoporphyrin-derivative (HpD)-treated lymphocytes. HpD is at present widely used as a tumor-locating and photosensitizing drug. It is therefore of great importance to study the extent to which the HpD uptake process depends on cell functional and...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/0009-2797(84)90090-5

    authors： Docchio F,Ramponi R,Sacchi CA,Bottiroli G,Freitas I

    更新日期：1984-07-01 00:00:00

  • Muscular dystrophy with laminin deficiency decreases acetylcholinesterase activity in thymus of dystrophic Lama2dy mice.

    abstract：:We have studied the effect of muscular dystrophy by merosin deficiency on mouse thymus acetyl- (AChE) and butyrylcholinesterase (BuChE). The organ contains AChE and BuChE activities. Merosin deficiency causes an important decrease (46%) in AChE specific activity. Thymus produces dimers, monomers and tetramers of AChE,...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2005.10.092

    authors： Sánchez del Campo L,Nieto-Cerón S,Muñoz-Delgado E,Vidal CJ,Campoy FJ

    更新日期：2005-12-15 00:00:00

  • Kinetics and molecular docking studies of cholinesterase inhibitors derived from water layer of Lycopodiella cernua (L.) Pic. Serm. (II).

    abstract：:Acetylcholinesterase (AChE) inhibitors increase the availability of acetylcholine in central cholinergic synapses and are the most promising drugs currently available for the treatment of Alzheimer's disease (AD). Our screening study indicated that the water fraction of the methanolic extract of Lycopodiella cernua (L...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2015.07.008

    authors： Hung TM,Lee JS,Chuong NN,Kim JA,Oh SH,Woo MH,Choi JS,Min BS

    更新日期：2015-10-05 00:00:00

  • Molecular cloning of neuropathy target esterase (NTE).

    abstract：:Covalent modification of NTE, a neuronal protein with serine esterase activity, by certain organophosphates (OP) initiates degeneration of long axons in the peripheral and central nervous system. Simple inhibition of NTE esterase activity does not initiate neuropathy; the latter requires aging of the OP bound to the c...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章,评审

    doi：10.1016/s0009-2797(99)00065-4

    authors： Glynn P,Read DJ,Lush MJ,Li Y,Atkins J

    更新日期：1999-05-14 00:00:00

  • The 3D structure of the anticancer prodrug CPT-11 with Torpedo californica acetylcholinesterase rationalizes its inhibitory action on AChE and its hydrolysis by butyrylcholinesterase and carboxylesterase.

    abstract：:The anticancer prodrug CPT-11 is a highly effective camptothecin analog that has been approved for the treatment of colon cancer. The 2.6 angstroms resolution crystal structure of its complex with Torpedo californica acetylcholinesterase (TcAChE) demonstrates that CPT-11 binds to TcAChE and spans its gorge similarly t...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/j.cbi.2005.10.016

    authors： Harel M,Hyatt JL,Brumshtein B,Morton CL,Wadkins RM,Silman I,Sussman JL,Potter PM

    更新日期：2005-12-15 00:00:00

  • Inhibition of human glutathione S-transferase P1-1 by the flavonoid quercetin.

    abstract：:In the present study, the inhibition of human glutathione S-transferase P1-1 (GSTP1-1) by the flavonoid quercetin has been investigated. The results show a time- and concentration-dependent inhibition of GSTP1-1 by quercetin. GSTP1-1 activity is completely inhibited upon 1 h incubation with 100 microM quercetin or 2 h...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/s0009-2797(02)00250-8

    authors： van Zanden JJ,Ben Hamman O,van Iersel ML,Boeren S,Cnubben NH,Lo Bello M,Vervoort J,van Bladeren PJ,Rietjens IM

    更新日期：2003-05-06 00:00:00

  • A new direct method for determining superoxide dismutase activity by measuring hydrogen peroxide formation.

    abstract：:A new, direct method for determining superoxide dismutase activity is presented in this study. This method is based on measurement of one of the products of the superoxide dismutase reaction, hydrogen peroxide. Hydrogen peroxide is quantitated using a coupled reaction where horseradish peroxidase catalyzes the formati...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/0009-2797(93)90112-c

    authors： Segura-Aguilar J

    更新日期：1993-01-01 00:00:00

  • The interactions of benzo(a)pyrene with cell membranes: uptake into Chinese hamster ovary (CHO) cells and fluorescence studies with isolated membranes.

    abstract：:The interactions of benzo(a)pyrene (B(a)P) with the cell surface membrane were studied by measuring B(a)P uptake into intact mammalian cells and by determining B(a)P fluorescence in the presence of isolated cell surface membranes. It was found that 0.19 mu-g B(a)P were taken up by 10-6 Chinese hamster ovary (CHO) cell...

    journal_title：Chemico-biological interactions

    pub_type： 杂志文章

    doi：10.1016/0009-2797(75)90061-7

    authors： Brunette DM,Katz M

    更新日期：1975-07-01 00:00:00