Abstract：
:Quantitative fluorescence-based methods have been developed to determine the nuclear concentration of polyamide-fluorescein conjugates in cell culture. Confocal laser scanning microscopy and flow cytometry techniques are utilized to plot calibration curves, from which the nuclear concentration can be interpolated. Upon treatment with polyamide, the concentration in the nucleus of live HeLa cells is calculated to be between 0.1 and 0.5microM, which is significantly lower than the 2microM dosage concentration. In contrast, the observed nuclear concentration in U251 cells is closer to the dosage concentration, indicating a cell line-specific increase in uptake for this class of compounds. Although confocal microscopy and flow cytometry generate disparate values, taken together these experiments suggest that the polyamide concentration inside the cell nucleus is lower than it is outside the cell.
journal_name
Bioorg Med Chem Lettjournal_title
Bioorganic & medicinal chemistry lettersauthors
Hsu CF,Dervan PBdoi
10.1016/j.bmcl.2008.05.063subject
Has Abstractpub_date
2008-11-15 00:00:00pages
5851-5issue
22eissn
0960-894Xissn
1464-3405pii
S0960-894X(08)00560-Xjournal_volume
18pub_type
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journal_title：Bioorganic & medicinal chemistry letters
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journal_title：Bioorganic & medicinal chemistry letters
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journal_title：Bioorganic & medicinal chemistry letters
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journal_title：Bioorganic & medicinal chemistry letters
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journal_title：Bioorganic & medicinal chemistry letters
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journal_title：Bioorganic & medicinal chemistry letters
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journal_title：Bioorganic & medicinal chemistry letters
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更新日期：1999-06-07 00:00:00
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journal_title：Bioorganic & medicinal chemistry letters
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更新日期：2007-05-15 00:00:00
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journal_title：Bioorganic & medicinal chemistry letters
pub_type： 杂志文章
doi：10.1016/j.bmcl.2009.04.120
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journal_title：Bioorganic & medicinal chemistry letters
pub_type： 杂志文章
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更新日期：1998-02-03 00:00:00
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journal_title：Bioorganic & medicinal chemistry letters
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更新日期：2010-09-15 00:00:00
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journal_title：Bioorganic & medicinal chemistry letters
pub_type： 杂志文章
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更新日期：2002-09-16 00:00:00
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journal_title：Bioorganic & medicinal chemistry letters
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更新日期：2007-04-15 00:00:00
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journal_title：Bioorganic & medicinal chemistry letters
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journal_title：Bioorganic & medicinal chemistry letters
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journal_title：Bioorganic & medicinal chemistry letters
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journal_title：Bioorganic & medicinal chemistry letters
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更新日期：2011-11-01 00:00:00
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journal_title：Bioorganic & medicinal chemistry letters
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journal_title：Bioorganic & medicinal chemistry letters
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更新日期：2011-08-15 00:00:00
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journal_title：Bioorganic & medicinal chemistry letters
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更新日期：2007-08-15 00:00:00
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journal_title：Bioorganic & medicinal chemistry letters
pub_type： 杂志文章
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更新日期：2016-10-01 00:00:00
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journal_title：Bioorganic & medicinal chemistry letters
pub_type： 杂志文章
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更新日期：2014-12-15 00:00:00
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journal_title：Bioorganic & medicinal chemistry letters
pub_type： 杂志文章
doi：10.1016/j.bmcl.2008.12.095
更新日期：2009-02-15 00:00:00
