A high yield multi-method extraction protocol for protein quantification in activated sludge.

Abstract:

:A multi-method extraction protocol based on mechanical, ionic and hydrophobic methods was investigated on two types of activated sludge samples. Extraction methods were chosen with regards to optimal protein yield without cell disruption. Sonication, EDTA and Tween extraction methods were selected and combined. The total amount of protein released by the multi-method protocol sums up to 191 and 264 mg equiv. BSA/g VSS for the two different sludge samples. Protocol repetition on the same sample showed that protein yield after each successive protocol fitted an exponential curve model. The total amount of extractable proteins was evaluated by model predictions, 423 and 516 mg equiv. BSA/g VSS for the two sludge samples. The multi-method extraction protocol appears relevant for harvesting a representative quantity of proteins from the original sample (45-49%), moreover the multi-method criterion of the protocol also offers a heterogeneous pool of proteins. Thus, further qualitative studies may not be biased by the extraction protocol.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Monique R,Elisabeth GN,Etienne P,Dominique L

doi

10.1016/j.biortech.2008.02.025

subject

Has Abstract

pub_date

2008-11-01 00:00:00

pages

7464-71

issue

16

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(08)00167-3

journal_volume

99

pub_type

杂志文章
  • Efficient production of androstenedione by repeated batch fermentation in waste cooking oil media through regulating NAD+/NADH ratio and strengthening cell vitality of Mycobacterium neoaurum.

    abstract::The bioprocess for producing androstenedione (AD) from phytosterols by using Mycobacterium neoaurum is hindered by nicotinamide adenine dinucleotides (NAD+ and NADH) ratio imbalance, insoluble substrate, and lengthy biotransformation period. This study aims to improve the efficiency of AD production through a combined...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2019.01.144

    authors: Zhou X,Zhang Y,Shen Y,Zhang X,Xu S,Shang Z,Xia M,Wang M

    更新日期:2019-05-01 00:00:00

  • Anaerobic bioaugmentation hydrolysis of selected nitrogen heterocyclic compound in coal gasification wastewater.

    abstract::Anaerobic bottle experiments were conducted in parallel for 120 consecutive days to perform the bioaugmentation hydrolysis of selected nitrogen heterocyclic compounds (NHCs) in coal gasification wastewater. Due to enhancement with PAC and Fe(OH)3@PAC, quinoline, pyridine, and indole were hydrolyzed more effectively. T...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2018.12.113

    authors: Shi J,Han Y,Xu C,Han H

    更新日期:2019-04-01 00:00:00

  • Triacetonamine formation in a bio-oil from fast pyrolysis of sewage sludge using acetone as the absorption solvent.

    abstract::A sewage sludge sample was pyrolyzed in a drop tube furnace at 500 degrees C and sweeping gas flow rate of 300cm(3)/min. Triacetonamine (TAA) was detected with GC/MS as major component in the resulting bio-oil using acetone as the absorption solvent and proven to be a product from the reaction of NH(3) in the bio-oil ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2010.01.031

    authors: Cao JP,Zhao XY,Morishita K,Li LY,Xiao XB,Obara R,Wei XY,Takarada T

    更新日期:2010-06-01 00:00:00

  • Correlation of microbial community structure with pollutants removal, sludge reduction and sludge characteristics in micro-aerobic side-stream reactor coupled membrane bioreactors under different hydraulic retention times.

    abstract::A micro-aerobic side-stream reactor (MSSR) was inserted in returned sludge line of a membrane bioreactor (MBR) for sludge reduction. Three MSSR coupled MBRs (M-MBRs) and an anoxic/oxic MBR (AO-MBR) were operated in parallel to investigate effects of micro-aerobic condition and hydraulic retention time (HRT) of MSSR (H...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2018.03.088

    authors: Cheng C,Zhou Z,Pang H,Zheng Y,Chen L,Jiang LM,Zhao X

    更新日期:2018-07-01 00:00:00

  • Graphitic biochar as a cathode electrocatalyst support for microbial fuel cells.

    abstract::Graphitic biochar (BC) was generated using high temperature gasification and alkaline post-treatment (BCw) of wood-based biomass. The BCw was evaluated as a manganese oxide electrocatalytic support (MnO/BCw) and microbial fuel cell (MFC) air cathode. Nano-structured MnO2 crystals were successfully immobilized on bioma...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2015.06.012

    authors: Huggins TM,Pietron JJ,Wang H,Ren ZJ,Biffinger JC

    更新日期:2015-11-01 00:00:00

  • InCl3-catalyzed conversion of carbohydrates into 5-hydroxymethylfurfural in biphasic system.

    abstract::InCl3, a water-compatible Lewis acid, was used for the conversion of microcrystalline cellulose to produce 5-hydroxymethylfurfural (HMF) in a H2O/THF biphasic system. Addition of NaCl increased the HMF yield significantly but suppressed the levulinic acid (LA) formation. The HMF yield of 39.7% was obtained in 2h at 20...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2014.09.077

    authors: Shen Y,Sun J,Yi Y,Li M,Wang B,Xu F,Sun R

    更新日期:2014-11-01 00:00:00

  • Bacterial community shift in response to a deep municipal tail wastewater treatment system.

    abstract::In previous studies, ozone oyster shell fixed-bed bioreactor and membrane bioreactor (OFBR-MBR) were developed for municipal tail wastewater treatment, and qualified good effects. This study mainly discussed the bacterial community shift in response to the treatment process of OFBR-MBR. Proteobacteria, Chloroflexi, Pl...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2019.02.099

    authors: Chen J,Yang Y,Liu Y,Tang M,Wang R,Zhang C,Jiang J,Jia C

    更新日期:2019-06-01 00:00:00

  • An enhanced anaerobic membrane bioreactor treating bamboo industry wastewater by bamboo charcoal addition: Performance and microbial community analysis.

    abstract::In this study, two anaerobic membrane bioreactors (AnMBRs) were operated for 150days to treat bamboo industry wastewater (BIWW), and one of them was enhanced with bamboo charcoal (B-AnMBR). During the steady period, average chemical oxygen demand (COD) removal efficiencies of 94.5±2.9% and 89.1±3.1% were achieved in B...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2016.08.057

    authors: Xia T,Gao X,Wang C,Xu X,Zhu L

    更新日期:2016-11-01 00:00:00

  • Second generation bioethanol production from Saccharum spontaneum L. ssp. aegyptiacum (Willd.) Hack.

    abstract::Saccharum (Saccharum spontaneum L. ssp. aegyptiacum (Willd.) Hack.), is a rapidly growing, wide ranging high-yield perennial, suitable for second generation bioethanol production. This study evaluated oxalic acid as a pretreatment for bioconversion. Overall sugar yields, sugar degradation products, enzymatic glucan hy...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2010.02.036

    authors: Scordia D,Cosentino SL,Jeffries TW

    更新日期:2010-07-01 00:00:00

  • Differential effects of nutrient limitations on biochemical constituents and docosahexaenoic acid production of Schizochytrium sp.

    abstract::Four nutrient limitation cultures, namely monosodium glutamate (MSG-L), phosphate (P-L), ammonium sulfate (NH4(+)-L) and double (D-L, MSG and P limitation) limited, were designed to study how cell growth and biochemical components of Schizochytrium sp. were affected by nutrient limitations. All limited conditions caus...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2014.02.106

    authors: Sun L,Ren L,Zhuang X,Ji X,Yan J,Huang H

    更新日期:2014-05-01 00:00:00

  • Simultaneous sulfide removal and electricity generation with corn stover biomass as co-substrate in microbial fuel cells.

    abstract::Microbial fuel cells (MFCs), representing a promising method to treat combined pollutants with energy recovery, were utilized to remove sulfide and recover power with corn stover filtrate (CSF) as the co-substrate in present study. A maximum power density of 744 mW/m(2) was achieved with sulfide removal of 91% during ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2013.03.167

    authors: Zhang J,Zhang B,Tian C,Ye Z,Liu Y,Lei Z,Huang W,Feng C

    更新日期:2013-06-01 00:00:00

  • Carboxylic acid functionalized sesame straw: A sustainable cost-effective bioadsorbent with superior dye adsorption capacity.

    abstract::This study prepared a carboxylic functionalized bioadsorbent that met the "4-E" criteria: Efficient, Economical, Environmentally friendly, and Easily-produced. Sesame straw (Sesamum indicum L.) was functionalized through treatment with citric acid (SSCA) and tartaric acid (SSTA). The products were examined for adsorpt...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2017.04.066

    authors: Feng Y,Liu Y,Xue L,Sun H,Guo Z,Zhang Y,Yang L

    更新日期:2017-08-01 00:00:00

  • Dechlorination of chlorophenols found in pulp bleach plant E-1 effluents by advanced oxidation processes.

    abstract::Studies were conducted on the response of 2,4,6-trichlorophenol (1), 2,3,4,5-tetrachloro-phenol (2) and 4,5-dichloroguaiacol (3) toward advanced oxidation processes, such as UV-, O2/UV-, H2O2/UV-, O3/UV- and O3-H2O2/UV-photolyses with irradiation of 254 nm photons. The compounds 1-3 are among the chlorophenols found i...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2004.08.011

    authors: Wang R,Chen CL,Gratzl JS

    更新日期:2005-05-01 00:00:00

  • Treatment of hydroponic wastewater by denitrification filters using plant prunings as the organic carbon source.

    abstract::This study investigated the feasibility of using pre-treated plant liquors as organic carbon sources for the treatment of hydroponic wastewater containing high nitrate-N (>300 mg N/L). The waste plant material was pre-treated to extract organic carbon-rich liquors. When this plant liquor was used as an organic carbon ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2007.07.009

    authors: Park JB,Craggs RJ,Sukias JP

    更新日期:2008-05-01 00:00:00

  • Bio-hythane production from microalgae biomass: Key challenges and potential opportunities for algal bio-refineries.

    abstract::The interest in microalgae for wastewater treatment and liquid bio-fuels production (i.e. biodiesel and bioethanol) is steadily increasing due to the energy demand of the ultra-modern technological world. The associated biomass and by-product residues generated from these processes can be utilized as a feedstock in an...

    journal_title:Bioresource technology

    pub_type: 杂志文章,评审

    doi:10.1016/j.biortech.2017.05.156

    authors: Ghimire A,Kumar G,Sivagurunathan P,Shobana S,Saratale GD,Kim HW,Luongo V,Esposito G,Munoz R

    更新日期:2017-10-01 00:00:00

  • Consolidated bioprocessing of sugarcane bagasse and chicken manure to ammonium carboxylates by a mixed culture of marine microorganisms.

    abstract::The MixAlco process, an example of consolidated bioprocessing (CBP), was utilized for anaerobic fermentation of 80% lime-treated sugarcane bagasse/20% chicken manure at 55 degrees Celsius by a mixed culture of marine microorganisms. NH(4)HCO(3) was recommended by this study to replace CaCO(3) buffer to neutralize the ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2009.11.104

    authors: Fu Z,Holtzapple MT

    更新日期:2010-04-01 00:00:00

  • Electricity production and microbial community in psychrophilic microbial fuel cells at 10 °C.

    abstract::Psychrophilic microbial fuel cell (PMFC) offers an alternative method for low temperature wastewater treatment, but is seldom reported. In this study, the two-chamber PMFC was constructed at 10 °C using acetate as an electron donor. The maximum voltage under external resistance of 1000 Ω was around 550 mV. The columbi...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2020.123680

    authors: Dai K,Sun T,Yan Y,Qian DK,Zhang W,Zhang F,Jianxiong Zeng R

    更新日期:2020-10-01 00:00:00

  • Intermediate pyrolysis of agro-industrial biomasses in bench-scale pyrolyser: Product yields and its characterization.

    abstract::Pyrolysis of woody biomass, agro-residues and seed was carried out at 500 ± 10 °C in a fixed bed pyrolyser. Bio-oil yield was found varying from 20.5% to 47.5%, whereas the biochar and pyrolysis gas ranged from 27.5% to 40% and 24.5% to 40.5%, respectively. Pyrolysis gas was measured for flame temperature along with C...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2015.02.006

    authors: Tinwala F,Mohanty P,Parmar S,Patel A,Pant KK

    更新日期:2015-01-01 00:00:00

  • Enzymatic saccharification and bioethanol production from Cynara cardunculus pretreated by steam explosion.

    abstract::The correct choice of the specific lignocellulosic biomass pretreatment allows obtaining high biomass conversions for biorefinery implementations and cellulosic bioethanol production from renewable resources. Cynara cardunculus (cardoon) pretreated by steam explosion (SE) was involved in second-generation bioethanol p...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2015.03.037

    authors: Fernandes MC,Ferro MD,Paulino AFC,Mendes JAS,Gravitis J,Evtuguin DV,Xavier AMRB

    更新日期:2015-06-01 00:00:00

  • The utility of Shewanella japonica for microbial fuel cells.

    abstract::Shewanella-containing microbial fuel cells (MFCs) typically use the fresh water wild-type strain Shewanella oneidensis MR-1 due to its metabolic diversity and facultative oxidant tolerance. However, S. oneidensis MR-1 is not capable of metabolizing polysaccharides for extracellular electron transfer. The applicability...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2010.06.078

    authors: Biffinger JC,Fitzgerald LA,Ray R,Little BJ,Lizewski SE,Petersen ER,Ringeisen BR,Sanders WC,Sheehan PE,Pietron JJ,Baldwin JW,Nadeau LJ,Johnson GR,Ribbens M,Finkel SE,Nealson KH

    更新日期:2011-01-01 00:00:00

  • Immobilization and stabilization of invertase on Cajanus cajan lectin support.

    abstract::Use of lectins as ligands for the immobilization and stabilization of glycoenzymes has immense application in enzyme research and industry. But their widespread use could be limited by the high cost of their production. In the present study preparation of a novel and inexpensive lectin support for use in the immobiliz...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/s0960-8524(01)00053-0

    authors: Ahmad S,Anwar A,Saleemuddin M

    更新日期:2001-09-01 00:00:00

  • A review of biochar-based catalysts for chemical synthesis, biofuel production, and pollution control.

    abstract::This review addresses the use of biochar as a green and versatile catalyst support for emerging high-end applications beyond soil remediation, including chemical synthesis and biodiesel production from biomass, and pollutant degradation in the environment. Their catalytic performances are comparable or even superior t...

    journal_title:Bioresource technology

    pub_type: 杂志文章,评审

    doi:10.1016/j.biortech.2017.06.163

    authors: Xiong X,Yu IKM,Cao L,Tsang DCW,Zhang S,Ok YS

    更新日期:2017-12-01 00:00:00

  • A comparative account of glucose yields and bioethanol production from separate and simultaneous saccharification and fermentation processes at high solids loading with variable PEG concentration.

    abstract::A process strategy to aid in optimal enzymatic hydrolysis through the addition of polyethylene glycol (PEG6000) was tested for separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF). Pretreated wheat straw at 30% solids (w/w) loading was enzymatically hydrolyzed with 0, 0.5...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2019.03.060

    authors: Kadhum HJ,Mahapatra DM,Murthy GS

    更新日期:2019-07-01 00:00:00

  • Fruity flavors from waste: A novel process to upgrade crude glycerol to ethyl valerate.

    abstract::Valeric acid and its ester derivatives are chemical compounds with a high industrial interest. Here we report a new approach to produce them from crude glycerol, by combining propionic acid fermentation with chain elongation. Propionic acid was produced by Propionibacterium acidipropionici (8.49 ± 1.40 g·L-1). In the ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2019.121574

    authors: Ganigué R,Naert P,Candry P,de Smedt J,Stevens CV,Rabaey K

    更新日期:2019-10-01 00:00:00

  • Enrichment of high activity nitrifers to enhance partial nitrification process.

    abstract::Partial nitrification (PN) process is an essential pre-treatment to Anammox process for nitrogen removal from ammonium-rich wastewaters. High activity nitrifiers (HANs) are considered as the key to high-rate PN process. The HANs could be successfully enriched at free ammonia (FA) concentrations of 10-20 mg NH(3)-NL(-1...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2010.04.080

    authors: Chen J,Zheng P,Yu Y,Mahmood Q,Tang C

    更新日期:2010-10-01 00:00:00

  • Influence of nitrogen sources on ethanol fermentation in an integrated ethanol-methane fermentation system.

    abstract::An integrated ethanol-methane fermentation system was proposed to resolve wastewater pollution in cassava ethanol production. In the integrated system, wastewater originating from ethanol distillation was treated by two-stage anaerobic digestion and then used in medium for the next batch of ethanol fermentation. Ammon...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2012.06.032

    authors: Wang K,Mao Z,Zhang C,Zhang J,Zhang H,Tang L

    更新日期:2012-09-01 00:00:00

  • Factors contributing to the poor bulk behavior of meat and bone meal and methods for improving these behaviors.

    abstract::Meat and bone meal (MBM), a product of the rendering industry, is a potential feedstock for numerous bio-based applications. Design of processing equipment for MBM is difficult due to MBM's bulk behaviors; it flows less easily than many other granular materials, and it tends to foul the surfaces of processing equipmen...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2006.09.053

    authors: Garcia RA,Flores RA,Mazenko CE

    更新日期:2007-11-01 00:00:00

  • Consolidated bioprocessing performance of bacterial consortium EMSD5 on hemicellulose for isopropanol production.

    abstract::Consolidated bioprocessing (CBP) of lignocellulose by bacterial consortium for isopropanol production is considered as the most promising strategy. However, low utilization of xylan caused by the complex sidechain structure remains inhibit the conversion of full-biomass. In this study, isopropanol production from diff...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2019.121965

    authors: Liu L,Yang J,Yang Y,Luo L,Wang R,Zhang Y,Yuan H

    更新日期:2019-11-01 00:00:00

  • Physical properties of peanut hull pellets.

    abstract::Peanut hull (peanut pods and waste materials from processing of peanuts) was pelleted through a 3/16-in. (4.76 mm) diameter die. The effect of change in moisture content (4.2-21.2%, wet basis) of the pellets (due to exposure of the pellets to low or high humidity storage environments) on physical properties were measu...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2007.02.041

    authors: Fasina OO

    更新日期:2008-03-01 00:00:00

  • Removal of metal and organic pollutants from wastewater by a sequential selective technique.

    abstract::In this study the application of a sequential selective system that combined biosorption with biodegradation was evaluated as a feasible process for the removal of Cr(VI) and m-cresol from effluents. Cr(VI) biosorption on pretreated chestnut shells showed 100% metal removal and modelling efforts demonstrated that the ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2016.02.036

    authors: Cobas M,Danko AS,Pazos M,Sanromán MA

    更新日期:2016-08-01 00:00:00