Abstract:
:A multi-method extraction protocol based on mechanical, ionic and hydrophobic methods was investigated on two types of activated sludge samples. Extraction methods were chosen with regards to optimal protein yield without cell disruption. Sonication, EDTA and Tween extraction methods were selected and combined. The total amount of protein released by the multi-method protocol sums up to 191 and 264 mg equiv. BSA/g VSS for the two different sludge samples. Protocol repetition on the same sample showed that protein yield after each successive protocol fitted an exponential curve model. The total amount of extractable proteins was evaluated by model predictions, 423 and 516 mg equiv. BSA/g VSS for the two sludge samples. The multi-method extraction protocol appears relevant for harvesting a representative quantity of proteins from the original sample (45-49%), moreover the multi-method criterion of the protocol also offers a heterogeneous pool of proteins. Thus, further qualitative studies may not be biased by the extraction protocol.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Monique R,Elisabeth GN,Etienne P,Dominique Ldoi
10.1016/j.biortech.2008.02.025subject
Has Abstractpub_date
2008-11-01 00:00:00pages
7464-71issue
16eissn
0960-8524issn
1873-2976pii
S0960-8524(08)00167-3journal_volume
99pub_type
杂志文章abstract::The bioprocess for producing androstenedione (AD) from phytosterols by using Mycobacterium neoaurum is hindered by nicotinamide adenine dinucleotides (NAD+ and NADH) ratio imbalance, insoluble substrate, and lengthy biotransformation period. This study aims to improve the efficiency of AD production through a combined...
journal_title:Bioresource technology
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更新日期:2019-05-01 00:00:00
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journal_title:Bioresource technology
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journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2015.06.012
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abstract::InCl3, a water-compatible Lewis acid, was used for the conversion of microcrystalline cellulose to produce 5-hydroxymethylfurfural (HMF) in a H2O/THF biphasic system. Addition of NaCl increased the HMF yield significantly but suppressed the levulinic acid (LA) formation. The HMF yield of 39.7% was obtained in 2h at 20...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2014.09.077
更新日期:2014-11-01 00:00:00
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journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2019.02.099
更新日期:2019-06-01 00:00:00
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journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2016.08.057
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journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2010.02.036
更新日期:2010-07-01 00:00:00
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journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2014.02.106
更新日期:2014-05-01 00:00:00
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journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2013.03.167
更新日期:2013-06-01 00:00:00
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journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2017.04.066
更新日期:2017-08-01 00:00:00
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journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2004.08.011
更新日期:2005-05-01 00:00:00
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journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2007.07.009
更新日期:2008-05-01 00:00:00
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journal_title:Bioresource technology
pub_type: 杂志文章,评审
doi:10.1016/j.biortech.2017.05.156
更新日期:2017-10-01 00:00:00
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journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2009.11.104
更新日期:2010-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.biortech.2020.123680
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journal_title:Bioresource technology
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doi:10.1016/j.biortech.2010.06.078
更新日期:2011-01-01 00:00:00
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journal_title:Bioresource technology
pub_type: 杂志文章
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journal_title:Bioresource technology
pub_type: 杂志文章,评审
doi:10.1016/j.biortech.2017.06.163
更新日期:2017-12-01 00:00:00
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journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2019.03.060
更新日期:2019-07-01 00:00:00
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journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2019.121574
更新日期:2019-10-01 00:00:00
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journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2010.04.080
更新日期:2010-10-01 00:00:00
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journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2012.06.032
更新日期:2012-09-01 00:00:00
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journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2006.09.053
更新日期:2007-11-01 00:00:00
abstract::Consolidated bioprocessing (CBP) of lignocellulose by bacterial consortium for isopropanol production is considered as the most promising strategy. However, low utilization of xylan caused by the complex sidechain structure remains inhibit the conversion of full-biomass. In this study, isopropanol production from diff...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2019.121965
更新日期:2019-11-01 00:00:00
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journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2007.02.041
更新日期:2008-03-01 00:00:00
abstract::In this study the application of a sequential selective system that combined biosorption with biodegradation was evaluated as a feasible process for the removal of Cr(VI) and m-cresol from effluents. Cr(VI) biosorption on pretreated chestnut shells showed 100% metal removal and modelling efforts demonstrated that the ...
journal_title:Bioresource technology
pub_type: 杂志文章
doi:10.1016/j.biortech.2016.02.036
更新日期:2016-08-01 00:00:00