Abstract：
:An essential oil from a lemon grass variety of Cymbopogon flexuosus (CFO) and its major chemical constituent sesquiterpene isointermedeol (ISO) were investigated for their ability to induce apoptosis in human leukaemia HL-60 cells because dysregulation of apoptosis is the hallmark of cancer cells. CFO and ISO inhibited cell proliferation with 48 h IC50 of approximately 30 and 20 microg/ml, respectively. Both induced concentration dependent strong and early apoptosis as measured by various end-points, e.g. annexinV binding, DNA laddering, apoptotic bodies formation and an increase in hypo diploid sub-G0 DNA content during the early 6h period of study. This could be because of early surge in ROS formation with concurrent loss of mitochondrial membrane potential observed. Both CFO and ISO activated apical death receptors TNFR1, DR4 and caspase-8 activity. Simultaneously, both increased the expression of mitochondrial cytochrome c protein with its concomitant release to cytosol leading to caspase-9 activation, suggesting thereby the involvement of both the intrinsic and extrinsic pathways of apoptosis. Further, Bax translocation, and decrease in nuclear NF-kappaB expression predict multi-target effects of the essential oil and ISO while both appeared to follow similar signaling apoptosis pathways. The easy and abundant availability of the oil combined with its suggested mechanism of cytotoxicity make CFO highly useful in the development of anti-cancer therapeutics.
journal_name
Chem Biol Interactjournal_title
Chemico-biological interactionsauthors
Kumar A,Malik F,Bhushan S,Sethi VK,Shahi AK,Kaur J,Taneja SC,Qazi GN,Singh Jdoi
10.1016/j.cbi.2007.10.003subject
Has Abstractpub_date
2008-02-15 00:00:00pages
332-47issue
3eissn
0009-2797issn
1872-7786pii
S0009-2797(07)00302-Xjournal_volume
171pub_type
杂志文章abstract：:This paper assesses the current knowledge of 1,3-butadiene as an atmospheric pollutant, considers measurement techniques and reviews available data on 1,3-butadiene monitoring and emissions estimates. Atmospheric chemistry, sources of emission, current legislation, measurement techniques and monitoring programmes for ...
journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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更新日期：1990-01-01 00:00:00
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journal_title：Chemico-biological interactions
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更新日期：2004-07-20 00:00:00
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journal_title：Chemico-biological interactions
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pub_type： 杂志文章,评审
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更新日期：2006-10-27 00:00:00
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journal_title：Chemico-biological interactions
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更新日期：2015-08-05 00:00:00
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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更新日期：2007-04-05 00:00:00
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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更新日期：2020-01-05 00:00:00
abstract：:Styrene oxide was reacted with deoxynucleosides and DNA in aqueous buffer at pH 7.4. The products were purified by HPLC, characterized by UV spectroscopy and by chemical ionization mass spectrometry. The main products identified were 7-alkyl-, N2-alkyl- and O6-alkyldeoxyguanosine, 1-alkyl-, and N6-alkyldeoxyadenosine,...
journal_title：Chemico-biological interactions
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abstract：:Due to its antiviral effects and its importance for all immunological functions, the administration of selenium is suggested as a supportive measure in early as well as in advanced stages of HIV-induced disease. Initial observations on the effects of selenium supplementation in HIV-infected patients indicate that sele...
journal_title：Chemico-biological interactions
pub_type： 杂志文章,评审
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更新日期：1994-06-01 00:00:00
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journal_title：Chemico-biological interactions
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更新日期：2006-04-15 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
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abstract：:Acetaldehyde (AA) and methylglyoxal (MG) are reactive, ubiquitous aldehydes, present in the environment and endogenously formed in animals and humans. They have both been shown to readily form DNA adducts under simulated physiological conditions. We report here on the use of cultured normal and SV40T antigen-immortali...
journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/s0009-2797(97)00107-5
更新日期：1998-01-30 00:00:00
