Abstract：
BACKGROUND:Cell death induced by intracellular glutathione depletion has been reported to be dependent on the presence of trace amounts of extracellular copper ions. Since little is known about the relationship between glutathione depletion and copper homeostasis, we have in the present study further investigated the role of low amounts of copper ions in glutathione depletion. METHODS:Glutathione turnover was investigated in HeLa and hepatoma cell cultures with normal and low cysteine content in the presence of copper ions (1 and 10micromol/L) and two other glutathione-stimulating agents (lipoic acid and mercury ions). RESULTS:Copper ions (10micromol/L) caused relatively small increases in total amount of glutathione (the sum of the intracellular and the extracellular amount of glutathione) in HeLa and hepatoma cell cultures with normal cysteine levels (420nmol/mL) compared to control cell cultures, whereas lipoic acid and mercury ions strongly increased total glutathione in both types of cell cultures. Lower amount of total glutathione was observed in cell cultures with a lower cysteine levels (84nmol/mL), which is similar to that in human plasma. A strongly decreased total amount of glutathione in the presence of copper ions was observed in hepatoma cell cultures with lower cysteine levels, whereas the other agents showed effects similar to those described for cell cultures with normal cysteine levels. CONCLUSION:Glutathione synthesis in hepatoma cell cultures is probably more sensitive to a low cysteine level than HeLa cell cultures, and the presence of copper ions further decreases the availability of cysteine probably by increasing the disulfide binding to cysteine residues in extracellular proteins, which causes a further decrease of total glutathione.
journal_name
Chem Biol Interactjournal_title
Chemico-biological interactionsauthors
Hultberg M,Isaksson A,Andersson A,Hultberg Bdoi
10.1016/j.cbi.2007.01.005subject
Has Abstractpub_date
2007-04-05 00:00:00pages
56-62issue
1eissn
0009-2797issn
1872-7786pii
S0009-2797(07)00016-6journal_volume
167pub_type
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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pub_type： 杂志文章
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
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更新日期：2017-10-01 00:00:00
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