Abstract:
:In order to investigate how human immunodeficiency virus (HIV) gains entry to the placenta, we have performed in vitro experiments in which highly purified trophoblast cells isolated from term human placentas were examined for their susceptibility to HIV infection. Trophoblast cells were exposed to cell-free HIV-1 for up to 24 h, after which the cultures were monitored by p24 antigen capture assay, reverse transcriptase assay, and electron microscopy for evidence of virus uptake and replication. None was found. In the second series of experiments, trophoblast cells were cocultured with HIV-infected MOLT-4 cells for 24 h, stained using an anti-HIV antibody, and examined by immunofluorescence microscopy. The MOLT cells were strongly positive, as expected, but many trophoblast colonies also showed a punctate staining pattern. Examination of similar cultures using the electron microscope revealed MOLT cells adherent to trophoblast but no evidence of cell-cell fusion. Virions were observed in coated pits at the trophoblast cell surface and in endosomes or multivesicular bodies in the cytoplasm. These observations are consistent with an endocytosis-mediated mechanism of virus entry. Virions were also observed budding from the trophoblast plasma membrane, indicating that these cells can support HIV replication. To our knowledge, these results show for the first time that HIV can infect placental trophoblast cells in vitro. The results suggest that the placenta could become infected with HIV by the interaction of virus-infected maternal lymphocytes with syncytiotrophoblast bordering the maternal blood in the intervillous space.
journal_name
AIDS Res Hum Retrovirusesjournal_title
AIDS research and human retrovirusesauthors
Douglas GC,Fry GN,Thirkill T,Holmes E,Hakim H,Jennings M,King BFdoi
10.1089/aid.1991.7.735subject
Has Abstractpub_date
1991-09-01 00:00:00pages
735-40issue
9eissn
0889-2229issn
1931-8405journal_volume
7pub_type
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1998.14.299
更新日期:1998-03-01 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1998.14.39
更新日期:1998-01-01 00:00:00
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journal_title:AIDS research and human retroviruses
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2007-02-01 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章,多中心研究
doi:10.1089/aid.1992.8.1919
更新日期:1992-11-01 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/088922202760019347
更新日期:2002-06-10 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/088922299311682
更新日期:1999-01-01 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1990.6.813
更新日期:1990-06-01 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1997.13.945
更新日期:1997-07-20 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.2006.0184
更新日期:2007-03-01 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/AID.2016.0128
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/08892220360473961
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1988.4.51
更新日期:1988-02-01 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.2015.0026
更新日期:2016-03-01 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1089/aid.1992.8.1321
更新日期:1992-08-01 00:00:00
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doi:10.1089/aid.2005.0072
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journal_title:AIDS research and human retroviruses
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
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更新日期:2014-06-01 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.1995.11.1467
更新日期:1995-12-01 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.2008.0154
更新日期:2008-12-01 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/08892220152741388
更新日期:2001-12-10 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/AID.2012.0018
更新日期:2012-12-01 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
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更新日期:2011-05-01 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/AID.2020.0150
更新日期:2020-11-10 00:00:00
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journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/088922204323087804
更新日期:2004-05-01 00:00:00
abstract::In HIV-infected persons, certain HLA class I alleles are associated with effective control of viremia, while others are associated with rapid disease progression. Among the most divergent clinical outcomes are the relatively good prognosis in HLA-B*5801 expressing persons and poor prognosis with HLA-B*5802. These two ...
journal_title:AIDS research and human retroviruses
pub_type: 杂志文章
doi:10.1089/aid.2007.0124
更新日期:2008-01-01 00:00:00