Abstract:
:The C-terminal fragment of Clostridium perfringens enterotoxin (C-CPE) modulates the barrier function of claudin-4 via its C-terminal 16 amino acids. In the current study, we investigated the roles of tyrosine residues (Y306, Y310 and Y312) in this region in the modulation of TJs by C-CPE. Single mutations of Y306, Y310 and Y312 to alanine resulted in partial reduction of claudin-4 binding. We also prepared double mutants of C-CPE to further evaluate the roles of these tyrosine residues. Replacement of Y310 and Y312 with alanine (Y310A/Y312A) partly reduced the ability of C-CPE to bind to claudin-4. Double mutants Y306A/Y310A and Y306A/Y312A, however, lost the ability to bind to claudin-4 and to modulate the TJ barrier. We also found that a triple mutant (Y306A/Y310A/Y312A) lost the ability to bind claudin-4, modulate the TJ barrier, and enhance jejunal absorption in rats. These results indicate that tyrosines 306, 310, and 312 are critical for the interaction of C-CPE with claudin-4 and for the modulation of TJ barrier function by C-CPE. This study provides information that should help in the development of claudin modulators based on C-CPE.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Harada M,Kondoh M,Ebihara C,Takahashi A,Komiya E,Fujii M,Mizuguchi H,Tsunoda S,Horiguchi Y,Yagi K,Watanabe Ydoi
10.1016/j.bcp.2006.10.002subject
Has Abstractpub_date
2007-01-15 00:00:00pages
206-14issue
2eissn
0006-2952issn
1873-2968pii
S0006-2952(06)00622-8journal_volume
73pub_type
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