Abstract:
:The formyl peptide receptor (FPR) gene family has a complex evolutionary history and comprises eight murine members but only three human representatives. To enable translation of results obtained in mouse models of human diseases, more comprehensive knowledge of the pharmacological similarities/differences between the human and murine FPR family members is required. Compared to FPR1 and FPR2 expressed by human neutrophils, very little is known about agonist/antagonist recognition patterns for their murine orthologues, but now we have identified two potent and selective formylated peptide agonists (fMIFL and PSMα2) for Fpr1 and Fpr2, respectively. These peptides were used to determine the inhibition profile of a set of antagonists with known specificities for the two FPRs in relation to the corresponding murine receptors. Some of the most potent and selective antagonists for the human receptors proved to be devoid of effect on their murine orthologues as determined by their inability to inhibit superoxide release from murine neutrophils upon stimulation with receptor-specific agonists. The Boc-FLFLF peptide was found to be a selective antagonist for Fpr1, whereas the lipidated peptidomimetic Lau-(Lys-βNSpe)6-NH2 and the hexapeptide WRW4 were identified as Fpr2-selective antagonists.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Skovbakke SL,Winther M,Gabl M,Holdfeldt A,Linden S,Wang JM,Dahlgren C,Franzyk H,Forsman Hdoi
10.1016/j.bcp.2016.09.004subject
Has Abstractpub_date
2016-11-01 00:00:00pages
56-65eissn
0006-2952issn
1873-2968pii
S0006-2952(16)30264-7journal_volume
119pub_type
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