Abstract:
:The signal transducer and activator of transcription 3 is a constitutively activated oncogenic protein in various human tumors and represents a valid target for anticancer drug design. In this study, we have achieved a new type of STAT3 inhibitors based on structural modifications on shikonin scaffold, guided by computational modelling. By tests, PMMB-187 exhibited a more outstanding profile than shikonin on a small panel of human breast cancer cells, especially for the MDA-MB-231 cells. For the cellular mechanisms research, PMMB-187 was found to induce cell apoptosis in MDA-MB-231 cells, associated with the reduction of mitochondrial membrane potential, production of ROS and alteration of the levels of apoptosis-related proteins. Furthermore, PMMB-187 inhibited constitutive/inducible STAT3 activation, transcriptional activity, nuclear translocation and downstream target genes expression in STAT3-dependent breast cancer cells MDA-MB-231. Besides, no obvious inhibitory effect on activation of STAT1 and STAT5 was observed with PMMB-187 treatment. Most notably, the in vivo studies further revealed that PMMB-187 could dramatically suppress the MDA-MB-231 cells xenografted tumor growth. The in vitro and in vivo results collectively suggest that PMMB-187 may serve as a promising lead compound for the further development of potential therapeutic anti-neoplastic agents.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Qiu HY,Fu JY,Yang MK,Han HW,Wang PF,Zhang YH,Lin HY,Tang CY,Qi JL,Yang RW,Wang XM,Zhu HL,Yang YHdoi
10.1016/j.bcp.2017.10.009subject
Has Abstractpub_date
2017-12-15 00:00:00pages
74-86eissn
0006-2952issn
1873-2968pii
S0006-2952(17)30639-1journal_volume
146pub_type
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