Abstract：
:We investigated the effect of aphidicolin, an inhibitor of DNA polymerase alpha and delta, on the induction of apoptosis by arabinosyl nucleosides in a human promyelocytic leukemia cell line, HL-60. Pretreatment of HL-60 cells with aphidicolin (2 microM) significantly increased the number of morphologically apoptotic cells induced by 1-beta-D arabinofuranosylcytosine (ara-C) during 4 hr of incubation. This is consistent with the appearance of DNA fragmentation as determined quantitatively by diphenylamine or by agarose gel electrophoresis. The inhibition of cell growth on day 3 after drug exposure was correlated with the degree of apoptosis: Such synergistic interaction between aphidicolin and ara-C has also been observed in other human myeloid leukemia cell lines, U937 and KG-1. In addition, the induction of apoptosis by 9-beta-D arabinofuranosyladenine or 9-beta-D arabinofuranosylguanine is augmented by aphidicolin.
journal_name
Biochem Pharmacoljournal_title
Biochemical pharmacologyauthors
Kuwakado K,Kubota M,Hirota H,Adachi S,Matsubara K,Kasai Y,Akiyama Y,Mikawa Hdoi
10.1016/0006-2952(93)90631-6subject
Has Abstractpub_date
1993-12-03 00:00:00pages
1909-16issue
11eissn
0006-2952issn
1873-2968pii
0006-2952(93)90631-6journal_volume
46pub_type
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