Abstract：
:Acute isolates of Coxiella burnetii possess a 36-kbp plasmid termed QpH1. DNA hybridizations show that QpH1 contains approximately 6-kbp region of DNA which is not present in the QpRS plasmid from chronic isolates. This QpH1-specific region of DNA contains the contiguous EcoRI fragments G, E, and D. The GED region was found to possess seven open reading frames (ORF's) coding for proteins ranging from 5.5 to 42.3 kDa in molecular mass when subcloned and expressed in vitro. Summing the predicted ORF's accounts for 95% of the GED coding potential. E. coli expression produced a stable 42.3-kDa protein from the pHIN19 subclone of GED. The ORF of the 42.3-kDa protein, termed cbhE', has been localized on GED by both in vitro transcription/translation and DNA sequencing. The cbhE' gene is estimated as 1142 bp in length with a putative promoter region of TCAACT (-35)-N16-TAAAAT (-10)-N14-AGAAGGA (Shine-Dalgarno)-N10-ATG.
journal_name
Acta Viroljournal_title
Acta virologicaauthors
Minnick MF,Small CL,Frazier ME,Mallavia LPsubject
Has Abstractpub_date
1991-11-01 00:00:00pages
497-502issue
6eissn
0001-723Xissn
1336-2305journal_volume
35pub_type
杂志文章相关文献
ACTA VIROLOGICA文献大全abstract：:Transfer factor specific for herpes simplex virus (HSV) type 1 (TFHSV-1) was prepared from splenic cells of HSV-1 immunized mice. Protection was transferred with TFHSV-1 to nonimmune mouse recipients. The TFHSV-1 injected mice had a higher survival rate after lethal HSV-1 challenge as compared to mice injected with a ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1987-11-01 00:00:00
abstract：:Homologous interference in vitro is defined as the ability of primary viral infection to prevent secondary homologous superinfection. Non-cytopathic bovine viral diarrhea virus (ncp BVDV) has been classified according to the exaltation of Newcastle disease phenomenon (END) as END positive (E+) and END negative (E-) st...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2018_228
更新日期：2018-01-01 00:00:00
abstract：:Some differences were established between mopyridone-sensitive (MCU-s) wild strain and mopyridone-resistant (MCU-r) mutant progenies of influenza virus A/Hong Kong/1/68 (H3N2). The virions of MCU-r mutant had a lower buoyant density in linear sucrose gradient as compared to those of MCU-s strain, and an increased abil...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：:In this study, a major part of genome of the pestivirus isolate 297 from Slovakia, comprising the 7195 nt-long 5΄-UTR-NS3 region was sequenced and analyzed. Conserved cleavage sites between individual viral proteins of this region were determined and the number of amino acids of respective proteins was estimated as fo...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2013_01_17
更新日期：2013-01-01 00:00:00
abstract：:Biological properties of antigens of whole cells of C. burnetii in phase I (WCI), their residue (CMR) and extract (CME) obtained by extraction of C. burnetti with chloroform/methanol mixture were subjected to a comparative study. It was shown that CME is able to react specifically with sera containing antibodies again...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
abstract：:Monoclonal antibodies (MAbs) were prepared against ICP4 of Marek's disease virus (MDV). Mice were inoculated with ICP4 obtained from High-Five insect cells infected with a recombinant baculovirus expressing ICP4. MAbs were selected by enzyme-linked immunosorbent assay (ELISA) using MDV-infected and control chick kidne...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
abstract：:In the last two decades, preparation of infectious RNA or DNA clones of plant viruses has transformed to a standard laboratory technique, providing an excellent tool for the research of viral gene functions and virus-host interactions. A full-length clone can be transformed in vitro to a viral vector expressing foreig...
journal_title：Acta virologica
pub_type： 杂志文章,评审
doi：
更新日期：2007-01-01 00:00:00
abstract：:RNA of partially purified Kemerovo virus sedimented in a relatively broad region in sucrose density gradient with a peak at 14 S. Approximately 65% of this RNA was resistant to the action of ribonuclease which indicates the double-stranded nature of Kemerovo virus RNA. ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1975-01-01 00:00:00
abstract：:A total of 2922 small terrestrial mammals of 12 species were collected in six localities of West Slovakia between 1981 and 1986. When examined for the prevalence of neutralizing antibodies (NA) to Central European encephalitis (CEE) virus we found that 14.6% had antibody. Nearly all (97%) of the 426 animals with antib...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:The lytic activity of splenocytes from C3H mice immunized with a highly attenuated line of Langat virus [tick-borne encephalitis (TBE) complex] was determined by the in vitro 51Cr release assay on TBE virus (western subtype)-infected target L929 cells. After the spleen cell suspensions were depleted of T cells with an...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:Hypothiocyanite (OSCN-) is a natural component of human saliva and is produced by the lactoperoxidase (LPO)/thiocyanate/hydrogen peroxide (H2O2) system. OSCN- has been previously shown to exhibit antiviral activity against influenza viruses (IFV) A/H1N1/2009 and A/H1N2/2009 in vitro as well as antimicrobial and antifu...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2018_408
更新日期：2018-01-01 00:00:00
abstract：:An analysis of the methods of testing the antiviral activity in vitro (in cell cultures) and in vivo (in experimental virus infections) is presented. The necessity of applying two-stage screening in vitro is emphasized. The chemotherapeutic viral models is in vitro testing as well as the principles of modelling of exp...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1978-07-01 00:00:00
abstract：:Cauliflower mosaic virus (CaMV) - a plant pararetrovirus that naturally causes diseases in Brassicaceae and Solanaceae plant hosts worldwide - has been detected by PCR for the first time in herbarium samples of Usnea sp. lichens. The virus's presence in these lichens did not result in any micro- or macromorphological ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2016_02_196
更新日期：2016-06-01 00:00:00
abstract：:Mouse polyclonal antibodies were prepared against broad bean stain virus (BBSV, Comovirus group) and its coat protein subunits, large (L) and small (S) protein. These subunits were less immunogenic than native virus. Antibodies against L protein (Anti-L) and native virus (Anti-BBSV) did not react in immunoblots with S...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:Plum pox virus (PPV) isolates are grouped into three clusters differentiated by biological, serological, molecular and epidemiological characteristics: Marcus (M), Dideron (D) and Cherry (C). The El Amar (EA) isolate that does not fit any of the above groups is also known. Monoclonal antibodies (MAbs) that specificall...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:Herpes simplex virus (HSV) DNA was found by spot blot hybridization in the right nc. amygdalae of 3 out 10 patients who underwent curative stereotactic surgery for severe mental retardation with aggressive behavior and/or paranoic schizophrenia. Of these, 6 were also tested for the presence of CMV DNA sequences with n...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1988-09-01 00:00:00
abstract：:The coat proteins of both ordinary (PVSO) and Andean (PVSA) strains were shown to be similar in molecular weights, peptide maps, amino acid analysis and reaction to a range of carlavirus antiseras. ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:Effects of certain groups of nutrients such as glucose, essential amino acids (AA), nonessential AA, vitamins and trace nutrients on the multiplication of various strains of Japanese encephalitis virus (JEV) were studied with an aim to optimise the conditions for cultivation of the virus in porcine stable (PS) kidney ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
abstract：:The equine influenza virus, Ludhiana/5/87, isolated from the clinical material during the epidemic of equine influenza in India in 1987 was inhibited in haemagglutination-inhibition test by the antiserum against the prototype A/eq/Prague/1/56 (H7N7) virus and by post-epidemic horse sera. In haemagglutinin and neuramin...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:Most adults are asymptomatically infected with Epstein-Barr virus (EBV). Primary EBV infection is commonly associated with acute infectious mononucleosis (IM). T cell immune activation plays an important role in EBV-associated diseases. IM shows a mainly Th1-type profile, so Th1-type cytokines such as interleukin-2 (I...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:The cold-adapted temperature-sensitive (ts) influenza virus strain A/Leningrad/134/17/57 (H2N2) multiplied well at 32 degrees C (optimal temperature); lower titres of infectious virus were obtained in developing chick embryos at 40 degrees C. In a canine kidney (MDCK) cell line and in primary calf kidney (CK) cells an...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1982-07-01 00:00:00
abstract：:Two monoclonal antibodies (MoAb) to the Skalica virus from the tick-borne encephalitis (TBE) complex were used to compare Karshi and Royal Farm viruses with the Russian spring-summer encephalitis, Central European encephalitis (Hypr and Kumlinge strains) Skalica, Langat and Powassan viruses. The first MoAb was prepare...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1990-02-01 00:00:00
abstract：:Every year, the poultry industry experiences significant economic losses due to epidemics of Newcastle disease virus (NDV). Developing new vaccines by identifying and using the immunogenic hemagglutinin-neuraminidase (HN) protein can protect the poultry industry. In the present study, the full-length HN protein was ex...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2020_310
更新日期：2020-01-01 00:00:00
abstract：:In the blood of patients with rheumatoid arthritis and/or sclerodermia systematica usually acid-labile interferon-alpha (IFN-alpha) was found. Blood leukocytes cannot be considered the source of its production as they spontaneously produce IFN-gamma identified with specific antiserum. Blood leukocytes of tested patien...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
abstract：:A peculiar grouping of Creutzfeldt-Jacob disease (CJD) and of other organic presenile dementia (OPD) cases in a small, prevalently rural area in Czechoslovakia to the east of 19 degrees 35' E and to the north of 48 degrees 15' N was studied. Between August, 1972 and November, 1976, three histologically proven CJD case...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1978-03-01 00:00:00
abstract：:This study comprised 100 persons with antibodies to hepatitis C virus (HCV), including 77 intravenous drug users (IVDUs). They were tested with serological HCV typing assays (Murex HCV serotyping 1-6 assay; Chiron RIBA HCV Serotyping SIA). Patients with a positive polymerase chain reaction (PCR) for HCV (n = 66) were ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1997-10-01 00:00:00
abstract：:A noninfectious soluble antigen fraction of goat poxvirus (GPV) fractionated by ammonium sulfate precipitation was tested for its suitability as coating antigen in an indirect enzyme-linked immunosorbent assay (ELISA). Accordingly, an avidin-biotin ELISA for the detection of GPV antibodies was optimized and evaluated ...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1999-10-01 00:00:00
abstract：:We attempted to quantify the protective potential of polyclonal IgG antibodies specific to the ectodomain of M2 protein (eM2) of influenza A virus (IAV) against lethal influenza infection of mice. For this purpose, eM2 conjugated with keyhole limpet hemocyanin (KLH) or KLH alone were administered with Freund's adjuvan...
journal_title：Acta virologica
pub_type： 杂志文章
doi：10.4149/av_2011_03_261
更新日期：2011-01-01 00:00:00
abstract：:Serum samples from 56 human immunodeficiency virus type 1 (HIV-1)-infected adult men were analysed for the presence of hepatitis B virus (HBV) serological markers. Two or more samples from each patient, collected over an interval of 1-6 years, were tested. The antibody against HBV core antigen (anti-HBc) prevalence wa...
journal_title：Acta virologica
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
abstract：:While the main interest in the pathogenesis of herpes simplex virus (HSV) in the sixties had been focussed on acute infections, in the seventies latent infection has become the main foal of investigation. Despite of overwhelming literature, the HSV persistence has remained a continuing problem from the practical as we...
journal_title：Acta virologica
pub_type： 杂志文章,评审
doi：
更新日期：1983-09-01 00:00:00
