当前位置： SCI文献检索 > CELLULAR SIGNALLING期刊下所有文献 > Inhibition of protein kinase C zeta blocks the attachment of stable microtubules to kinetochores leading to abnormal chromosome alignment.

Inhibition of protein kinase C zeta blocks the attachment of stable microtubules to kinetochores leading to abnormal chromosome alignment.

Abstract：

:The attachment of spindle microtubules to kinetochores is crucial for accurate segregation of chromosomes to daughter cells during mitosis. While a growing number of proteins involving this step are being identified, its molecular mechanisms are still not clear. Here we show that protein kinase C zeta (PKCzeta) is localized at the mitotic spindle during mitosis and plays a role in stable kinetochore-microtubule attachment. Striking staining for PKCzeta was observed at the mitotic spindle and spindle poles in cells at prometaphase and metaphase. PKCzeta molecules at these stages were phosphorylated at Thr-410, as detected by a phosphospecific antibody. PKCzeta was also detected at the spindle midzone and the midbody during anaphase and telophase, respectively, and PKCzeta at these stages was no longer phosphorylated at Thr-410. The polarity determinants Par3 and Par6, which are known to associate with PKCzeta, were also localized to the spindles and spindle poles at prometaphase and metaphase. Knockdown of PKCzeta by RNA interference affected normal chromosome alignment leading to generation of cells with aberrant nuclei. A specific PKCzeta inhibitor strongly blocked the formation of cold-sensitive stable kinetochore microtubules, and thus prevented microtubule-kinetochore attachment. Treatment of cells with the PKCzeta inhibitor also dislocated the minus-end directed motor protein dynein from kinetochores, but not the mitotic checkpoint proteins Mad2 and CENP-E. Prolonged exposure to the PKCzeta inhibitor eventually resulted in cell death. These results suggest a critical role of PKCzeta in spindle microtubule-kinetochore attachment and subsequent chromosomal separation.

journal_name

Cell Signal

journal_title

Cellular signalling

authors

Liu XF,Xie X,Miki T

doi

10.1016/j.cellsig.2006.05.017

subject

Has Abstract

pub_date

2006-12-01 00:00:00

pages

2314-23

issue

12

eissn

0898-6568

issn

1873-3913

pii

S0898-6568(06)00118-5

journal_volume

18

pub_type

杂志文章

相关文献

CELLULAR SIGNALLING文献大全
  • A cell surface inactive mutant of the human lutropin receptor (hLHR) attenuates signaling of wild-type or constitutively active receptors via heterodimerization.

    abstract：:The D405N and Y546F mutations of the human lutropin receptor (hLHR) have previously been shown to partially attenuate hCG-stimulated cAMP synthesis despite normal cell surface expression and hCG binding affinity (Min, L. and Ascoli, M. Mol. Endocrinol. 14:1797-1810, 2000). We now show that these mutations each stabili...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2009.07.003

    authors： Zhang M,Feng X,Guan R,Hébert TE,Segaloff DL

    更新日期：2009-11-01 00:00:00

  • cGMP-dependent protein kinase anchoring by IRAG regulates its nuclear translocation and transcriptional activity.

    abstract：:Type I cGMP-dependent protein kinases (PKGs) translocate to the nucleus to regulate gene expression in some, but not all cell types; we hypothesized that nuclear translocation of PKG may be regulated by extra-nuclear anchoring proteins. The inositol 1,4,5-triphosphate (IP(3)) receptor-associated cGMP kinase substrate ...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2008.03.009

    authors： Casteel DE,Zhang T,Zhuang S,Pilz RB

    更新日期：2008-07-01 00:00:00

  • Lyn contributes to regulation of multiple Kit-dependent signaling pathways in murine bone marrow mast cells.

    abstract：:SCF induces autophosphorylation of Kit and activates a variety of signaling components including Jnks, Erks, PI 3 Kinase, the JAK-Stat pathway and members of the Src family. Previously we showed that Lyn is activated at multiple points during SCF-induced cell cycle progression and contributes to SCF-mediated growth, c...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2004.06.004

    authors： Shivakrupa R,Linnekin D

    更新日期：2005-01-01 00:00:00

  • MyD88, IRAK1 and TRAF6 knockdown in human chondrocytes inhibits interleukin-1-induced matrix metalloproteinase-13 gene expression and promoter activity by impairing MAP kinase activation.

    abstract：:Interleukin-1 (IL-1) is the major prototypic proinflammatory cytokine that stimulates degradation of cartilage in arthritis by inducing prominent collagen II-degrading matrix metalloproteinase-13 (MMP-13). Nothing is known about the involvement of adaptor proteins, MyD88, IRAK1 and TRAF6 in MMP-13 regulation. Here we ...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2007.08.013

    authors： Ahmad R,Sylvester J,Zafarullah M

    更新日期：2007-12-01 00:00:00

  • Activation of phosphoinositide 3-kinase in response to inflammation and nitric oxide leads to the up-regulation of cyclooxygenase-2 expression and subsequent cell proliferation in mesangial cells.

    abstract：:In this study, we showed that nitric oxide (NO) donors induced the mesangial cell proliferation and cyclooxygenase-2 (COX-2) protein expression in murine mesangial cells. An inflammatory condition [lipopolysaccharide (LPS) plus interferon-gamma (IFN-gamma)] could also induce cell proliferation and significantly enhanc...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2004.11.015

    authors： Sheu ML,Chao KF,Sung YJ,Lin WW,Lin-Shiau SY,Liu SH

    更新日期：2005-08-01 00:00:00

  • Plasma membrane p180, which insulin receptor phosphorylates in vivo, is not a tyrosine kinase.

    abstract：:The earliest substrates to the transmembrane insulin receptor tyrosine kinase, that would function in insulin signalling, are likely to be associated with the plasma membrane. Rat liver plasma membrane 180,000 M(r) protein (p180) is a substrate to the insulin receptor in vitro [Goren et al. (1990) Cellular Signalling ...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/0898-6568(93)90016-f

    authors： Goren HJ,Boland D,Fei Q

    更新日期：1993-05-01 00:00:00

  • RhoA/ROCK I signalling downstream of the P2Y13 ADP-receptor controls HDL endocytosis in human hepatocytes.

    abstract：:Cell surface receptors for high-density lipoprotein (HDL) on hepatocytes are major partners in the regulation of cholesterol homeostasis. We have previously demonstrated on human hepatocytes that apolipoprotein A-I binding to an ectopic F(1)-ATPase stimulates the production of extracellular ADP that activates a P2Y(13...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2008.09.016

    authors： Malaval C,Laffargue M,Barbaras R,Rolland C,Peres C,Champagne E,Perret B,Tercé F,Collet X,Martinez LO

    更新日期：2009-01-01 00:00:00

  • Differential regulation of RhoA-mediated signaling by the TPalpha and TPbeta isoforms of the human thromboxane A2 receptor: independent modulation of TPalpha signaling by prostacyclin and nitric oxide.

    abstract：:In humans, thromboxane (TX) A(2) signals through the TPalpha and TPbeta isoforms of the TXA(2) receptor that exhibit common and distinct roles. For example, Gq/phospholipase (PL)Cbeta signaling by TPalpha is directly inhibited by the vasodilators prostacyclin and nitric oxide (NO) whereas that signaling by TPbeta is u...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2008.04.006

    authors： Wikström K,Kavanagh DJ,Reid HM,Kinsella BT

    更新日期：2008-08-01 00:00:00

  • Metformin triggers apoptosis in PEL cells and alters bortezomib-induced Unfolded Protein Response increasing its cytotoxicity and inhibiting KSHV lytic cycle activation.

    abstract：:Metformin, the most used drug for the treatment of diabetes type 2 patients, has been shown to have anti-cancer properties. In this study, we found that metformin induced apoptosis in Primary Effusion Lymphoma (PEL) cells, an aggressive B cell lymphoma associated with KSHV against which the conventional therapies usua...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2017.09.020

    authors： Granato M,Gilardini Montani MS,Romeo MA,Santarelli R,Gonnella R,D'Orazi G,Faggioni A,Cirone M

    更新日期：2017-12-01 00:00:00

  • Loss of Reelin protects mice against arterial thrombosis by impairing integrin activation and thrombus formation under high shear conditions.

    abstract：:Reelin is a secreted glycoprotein and essential for brain development and plasticity. Recent studies provide evidence that Reelin modifies platelet actin cytoskeletal dynamics. In this study we sought to dissect the contribution of Reelin in arterial thrombus formation. Here we analyzed the impact of Reelin in arteria...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2017.09.016

    authors： Gowert NS,Krüger I,Klier M,Donner L,Kipkeew F,Gliem M,Bradshaw NJ,Lutz D,Köber S,Langer H,Jander S,Jurk K,Frotscher M,Korth C,Bock HH,Elvers M

    更新日期：2017-12-01 00:00:00

  • CIKS (Act1 or TRAF3IP2) mediates high glucose-induced endothelial dysfunction.

    abstract：:Hyperglycemia-induced endothelial dysfunction is characterized by enhanced inflammatory cytokine and adhesion molecule expression, and endothelial-monocyte adhesion. The adapter molecule CIKS (connection to IKK and SAPK/JNK; also known as Act1 or TRAF3IP2) is an upstream regulator of NF-κB and AP-1, and plays a role i...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2012.10.009

    authors： Venkatesan B,Valente AJ,Das NA,Carpenter AJ,Yoshida T,Delafontaine JL,Siebenlist U,Chandrasekar B

    更新日期：2013-01-01 00:00:00

  • Evidence for stimulation of adenylyl cyclase by an activated G(s) heterotrimer in cell membranes: an experimental method for controlling the G(s) subunit composition of cell membranes.

    abstract：:Heterotrimeric (alphabetagamma) G(s) mediates agonist-induced stimulation of adenylyl cyclase (AC). Cholera toxin (CTx) will ADP-ribosylate the alpha-subunit of G(s) (G(s)alpha). G(s)alpha-deficient cyc(-) membranes were "stripped" of Gbeta. When the stripped cyc(-) were incubated with G(s)alpha and/or Gbetagamma, eac...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/s0898-6568(99)00078-9

    authors： Ganpat MM,Nishimura M,Toyoshige M,Okuya S,Pointer RH,Rebois RV

    更新日期：2000-02-01 00:00:00

  • Signaling networks of Rho GTPases in cell motility.

    abstract：:The last decades have witnessed an exponential increase in our knowledge of Rho GTPase signaling network which further highlighted the cross talk between these proteins and the complexity of their signaling pathways. In this review, we summarize the upstream and downstream players from Rho GTPases that are mainly invo...

    journal_title：Cellular signalling

    pub_type： 杂志文章,评审

    doi：10.1016/j.cellsig.2013.04.009

    authors： Hanna S,El-Sibai M

    更新日期：2013-10-01 00:00:00

  • GPCRs: Emerging anti-cancer drug targets.

    abstract：:G protein-coupled receptors (GPCRs) constitute the largest and most diverse protein family in the human genome with over 800 members identified to date. They play critical roles in numerous cellular and physiological processes, including cell proliferation, differentiation, neurotransmission, development and apoptosis...

    journal_title：Cellular signalling

    pub_type： 杂志文章,评审

    doi：10.1016/j.cellsig.2017.09.005

    authors： Nieto Gutierrez A,McDonald PH

    更新日期：2018-01-01 00:00:00

  • Role of glycosyl-phosphatidylinositol hydrolysis as a mitogenic signal for epidermal growth factor.

    abstract：:We have investigated the role of the hydrolysis of glycosyl-phosphatidylinositol (GPI) as one of the signalling pathways elicited after interaction of epidermal growth factor (EGF) with its specific plasma membrane receptor (EGFR). Endogenous GPI was characterized in both NIH 3T3 mouse fibroblast cells and in EGFR-tra...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/0898-6568(95)00002-7

    authors： Clemente R,Jones DR,Ochoa P,Romero G,Mato JM,Varela-Nieto I

    更新日期：1995-05-01 00:00:00

  • Studies on the effect of diadenlyated nucleotides on calcium mobilization and prostacyclin synthesis in bovine aortic endothelial cells.

    abstract：:Extracellular adenine dinucleotides are modulators of blood vessel tone. We have previously demonstrated that Ap(2)A and Ap(4)A induce the synthesis of nitric oxide (NO) from bovine aortic endothelial cells (BAEC) while Ap(3)A and Ap(5)A do not [FEBS Lett. 427 (1998) 320; Arch. Biochem. Biophys. 364 (1999) 280.]. In t...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/s0898-6568(00)00147-9

    authors： Haghiac M,Pojoga LH,Hilderman RH

    更新日期：2001-02-01 00:00:00

  • The functions of mechanosensitive ion channels in tooth and bone tissues.

    abstract：:Tooth and bone are independent tissues with a close relationship. Both are composed of a highly calcified outer structure and soft inner tissue, and both are constantly under mechanical stress. In particular, the alveolar bone and tooth constitute an occlusion system and suffer from masticatory and occlusal force. Thu...

    journal_title：Cellular signalling

    pub_type： 杂志文章,评审

    doi：10.1016/j.cellsig.2020.109877

    authors： Pei F,Liu J,Zhang L,Pan X,Huang W,Cen X,Huang S,Jin Y,Zhao Z

    更新日期：2021-02-01 00:00:00

  • The human stress-activated protein kinase-interacting 1 gene encodes JNK-binding proteins.

    abstract：:The orthologous proteins of the stress-activated protein kinase-interacting 1 (Sin1) family have been implicated in several different signal transduction pathways. In this study, we have investigated the function of the full-length human Sin1 protein and a C-terminally truncated isoform, Sin1alpha, which is produced b...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2004.10.015

    authors： Schroder W,Bushell G,Sculley T

    更新日期：2005-06-01 00:00:00

  • Proteasome inhibitor-I enhances tunicamycin-induced chemosensitization of prostate cancer cells through regulation of NF-κB and CHOP expression.

    abstract：:Although endoplasmic reticulum (ER) stress induction by some anticancer drugs can lead to apoptotic death of cancer cells, combination therapy with other chemicals would be much more efficient. It has been reported that proteasome inhibitors could induce cancer cell death through ER-stress. Our study, however, showed ...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2011.01.010

    authors： Huong PT,Moon DO,Kim SO,Kim KE,Jeong SJ,Lee KW,Lee KS,Jang JH,Erikson RL,Ahn JS,Kim BY

    更新日期：2011-05-01 00:00:00

  • Inter- and intra-cellular mechanism of NF-kB-dependent survival advantage and clonal expansion of radio-resistant cancer cells.

    abstract：:Understanding the underlying mechanism by which cancer cells acquire resistance to radiation and favorably selected for its clonal expansion will provide molecular insight into tumor recurrence at the treatment site. In the present study, we investigated the molecular mechanisms prompted in MCF-7 breast cancer cells i...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2017.01.011

    authors： Yu H,Aravindan N,Xu J,Natarajan M

    更新日期：2017-02-01 00:00:00

  • Different sensitivities of neutrophil responses to a selective protein kinase C inhibitor Ro 31-8425; redundancy in signal transduction.

    abstract：:Previous studies implicating a role for protein kinase C (PKC) in mediating stimulation of cellular responses by physiological agonists have relied on use of non-specific inhibitors or direct stimulation of PKC by phorbol esters. However, much of this evidence is questionable. Here, we have investigated the effects of...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/s0898-6568(96)00097-6

    authors： Merritt JE,Sullivan JA,Tse J,Wilkinson Se,Nixon JS

    更新日期：1997-01-01 00:00:00

  • KLF4 overcomes tamoxifen resistance by suppressing MAPK signaling pathway and predicts good prognosis in breast cancer.

    abstract：:Tamoxifen resistance represents a daunting challenge to the successful treatment for breast cancer. Krüppel-like factor 4 has critical roles in the development and progression of breast cancer, but its expression, function and regulation in the efficacy of TAM therapy in breast cancer have yet to be investigated. Here...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2017.09.025

    authors： Jia Y,Zhou J,Luo X,Chen M,Chen Y,Wang J,Xiong H,Ying X,Hu W,Zhao W,Deng W,Wang L

    更新日期：2018-01-01 00:00:00

  • A phospho-proteomic screen identifies novel S6K1 and mTORC1 substrates revealing additional complexity in the signaling network regulating cell growth.

    abstract：:S6K1, a critical downstream substrate of mTORC1, has been implicated in regulating protein synthesis and a variety of processes that impinge upon cell growth and proliferation. While the role of the cytoplasmic p70(S6K1) isoform in the regulation of translation has been intensively studied, the targets and function of...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2011.03.016

    authors： Jastrzebski K,Hannan KM,House CM,Hung SS,Pearson RB,Hannan RD

    更新日期：2011-08-01 00:00:00

  • Modifications of p53 protein and accumulation of p21 and gadd45 mRNA in TGF-beta 1 growth inhibited cells.

    abstract：:Transforming growth factory beta (TGF-beta) is a potent growth inhibitor of epithelial cells. One of the strategies used to elucidate the anti-proliferative mode of action of TGF-beta is to find out whether the receptor-generated signals interact with components of the basic machinery of the cell cycle. In this study ...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/s0898-6568(97)89890-7

    authors： Landesman Y,Bringold F,Milne DD,Meek DW

    更新日期：1997-05-01 00:00:00

  • Protein kinase C and annexins: unusual calcium response elements in the cell.

    abstract：:Protein kinase C and the annexins appear to share some unusual and potentially important membrane- and calcium-binding properties. While these proteins are calcium response elements, they are not calcium-binding proteins in the formal sense; at intracellular calcium concentrations, they only bind significant amounts o...

    journal_title：Cellular signalling

    pub_type： 杂志文章,评审

    doi：10.1016/0898-6568(93)90075-w

    authors： Bazzi MD,Nelsestuen GL

    更新日期：1993-07-01 00:00:00

  • Activated EGF receptor may balance ERK-inhibitory network signalling pathways.

    abstract：:In the COS-7 cell signalling network high levels of cAMP produced, for example, by co-stimulation of beta2-adrenergic receptor (beta2-AR) and bradykinin B2 receptor (BKR) may affect epidermal growth factor receptor (EGFR)-mediated activation of extracellular signal-stimulated kinase (ERK). In contrast, co-stimulation ...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2005.08.019

    authors： Hanke S,Valkova C,Stirnweiss J,Drube S,Liebmann C

    更新日期：2006-07-01 00:00:00

  • Thrombin induces nestin expression via the transactivation of EGFR signalings in rat vascular smooth muscle cells.

    abstract：:Regulation of nestin gene expression is largely unknown despite that it is widely used as a progenitor cell marker. In this study, we showed that nestin expression is regulated by the thrombin-mediated EGFR transactivation in serum-deprived primary cultures of rat vascular smooth muscle cells (VSMCs). This resulted fr...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2009.02.005

    authors： Huang YL,Shi GY,Lee H,Jiang MJ,Huang BM,Wu HL,Yang HY

    更新日期：2009-06-01 00:00:00

  • Genistein inhibits potentiation by wortmannin of protein kinase C-activated phospholipase D in osteoblast-like cells.

    abstract：:We previously showed that protein kinase C (PKC) induces phosphatidylcholine-hydrolysing phospholipase D activation in osteoblast-like MC3T3-E1 cells and that tyrosine kinase is involved in this activation. Wortmannin, a potent inhibitor of phosphatidylinositol 3-kinase, markedly enhanced the formation of choline indu...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/0898-6568(94)00080-u

    authors： Kozawa O,Suzuki A,Shinoda J,Oiso Y

    更新日期：1995-03-01 00:00:00

  • Gax inhibits perivascular preadipocyte biofunction mediated by IGF-1 induced FAK/Pyk2 and ERK2 cooperative pathways.

    abstract：:Perivascular adipocyte (PVAC) biofunctions were closely related to cardiovascular diseases; its specific biological mechanisms remained unclear. How to adjust PVAC functions of vascular cells is an important topic. The present study was designed to investigate whether FAK/Pyk2 and ERK1/2 MAPK signaling pathways partic...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/j.cellsig.2014.09.017

    authors： Liu P,Feng J,Kong F,Lu Q,Xu H,Meng J,Jiang Y

    更新日期：2014-12-01 00:00:00

  • Increase of [Ca(2+)](i) via activation of ATP receptors in PC-Cl3 rat thyroid cell line.

    abstract：:In PC-Cl3 rat thyroid cell line, ATP and UTP provoked a transient increase in [Ca(2+)](i), followed by a lower sustained phase. Removal of extracellular Ca(2+) reduced the initial transient response and completely abolished the plateau phase. Thapsigargin (TG) caused a rapid rise in [Ca(2+)](i) and subsequent addition...

    journal_title：Cellular signalling

    pub_type： 杂志文章

    doi：10.1016/s0898-6568(01)00208-x

    authors： Marsigliante S,Elia MG,Di Jeso B,Greco S,Muscella A,Storelli C

    更新日期：2002-01-01 00:00:00