N-linked glycosylation of a beetle (Apriona germari) cellulase Ag-EGase II is necessary for enzymatic activity.

Abstract:

:We previously reported that the beta-1,4-endoglucanase (EGase) belonging to glycoside hydrolase family (GHF) 45 of the mulberry longicorn beetle, Apriona germari (Ag-EGase II), has three potential N-linked glycosylation sites; these sites are located at amino acid residues 56-59 (NKSG), 99-102 (NSTF), and 237-239 (NYSstop). In the present study, we analyze the functional role of these potential N-linked glycosylation sites. Tunicamycin treatment completely abolished the enzymatic activity of Ag-EGase II. To further elucidate the functional role of the N-linked glycosylation sites in Ag-EGase II, we have assayed the cellulase enzyme activity in Ser58Gln, Thr101Gln, or Ser239Gln mutants. Lack of N-linked glycosylation site at residues 99-102 (NSTF), the site of which is conserved in known beetle GHF 45 cellulases, showed loss of enzyme activity and reduced the molecular mass of the enzyme. In contrast, mutations in Ser58Gln or Ser239Gln affected neither the activity nor the apparent molecular mass of the enzyme, indicating that these sites did not lead to N-linked glycosylation. The present study demonstrates that N-linked glycosylation at residues 99-102 (NSTF), while not essential for secretion, is required for Ag-EGase II enzyme activity.

authors

Wei YD,Lee KS,Gui ZZ,Yoon HJ,Kim I,Je YH,Lee SM,Zhang GZ,Guo X,Sohn HD,Jin BR

doi

10.1016/j.ibmb.2006.03.007

subject

Has Abstract

pub_date

2006-06-01 00:00:00

pages

435-41

issue

6

eissn

0965-1748

issn

1879-0240

pii

S0965-1748(06)00071-3

journal_volume

36

pub_type

杂志文章
  • LIM-homeodomain transcription factor Awh is a key component activating all three fibroin genes, fibH, fibL and fhx, in the silk gland of the silkworm, Bombyx mori.

    abstract::In the silkworm Bombyx mori, three fibroin genes, fibroin-heavy-chain (fibH), fibroin-light-chain (fibL) and fibrohexamerin (fhx), are coexpressed only in the posterior silk gland (PSG) cells, while the sericin genes encoding silk glue proteins are expressed in the middle silk gland (MSG) cells. Silk gland factor-2 (S...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2014.11.003

    authors: Kimoto M,Tsubota T,Uchino K,Sezutsu H,Takiya S

    更新日期:2015-01-01 00:00:00

  • Considerations on the structural evidence of a ligand-binding function of ultraspiracle, an insect homolog of vertebrate RXR.

    abstract::This analysis considers the structural evidence of a ligand-binding function of the nuclear receptor ultraspiracle (USP). The positions and nature of residues in the ligand-binding domain of USP from six higher insects is evaluated in comparison to the function of conserved residues vertebrate receptors that have been...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章,评审

    doi:10.1016/s0965-1748(00)00038-2

    authors: Jones G,Jones D

    更新日期:2000-08-01 00:00:00

  • Two single mutations commonly cause qualitative change of nonspecific carboxylesterases in insects.

    abstract::Carboxylesterases provide key mechanisms of resistance to insecticides, particularly organophosphates (OPs), in insects. One resistance mechanism is a qualitative change in the properties of a carboxylesterase. Two mutant forms, G151D and W271L, have been observed, mostly in dipteran species, to affect substrate speci...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2010.09.004

    authors: Cui F,Lin Z,Wang H,Liu S,Chang H,Reeck G,Qiao C,Raymond M,Kang L

    更新日期:2011-01-01 00:00:00

  • Silencing the odorant receptor co-receptor RproOrco affects the physiology and behavior of the Chagas disease vector Rhodnius prolixus.

    abstract::Olfaction is one of the main sensory modalities that allow insects to interpret their environment. Several proteins, including odorant-binding proteins (OBPs) and odorant receptors (ORs), are involved in this process. Odorant receptors are ion channels formed by a binding unit OR and an odorant receptor co-receptor (O...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2015.02.012

    authors: Franco TA,Oliveira DS,Moreira MF,Leal WS,Melo AC

    更新日期:2016-02-01 00:00:00

  • Insect natural products and processes: new treatments for human disease.

    abstract::In this overview, some of the more significant recent developments in bioengineering natural products from insects with use or potential use in modern medicine are described, as well as in utilisation of insects as models for studying essential mammalian processes such as immune responses to pathogens. To date, insect...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.ibmb.2011.05.007

    authors: Ratcliffe NA,Mello CB,Garcia ES,Butt TM,Azambuja P

    更新日期:2011-10-01 00:00:00

  • A RhoGAP protein as a main immune suppressive factor in the Leptopilina boulardi (Hymenoptera, Figitidae)-Drosophila melanogaster interaction.

    abstract::To protect its eggs, the endoparasitoid wasp Leptopilina boulardi injects immune suppressive factors into Drosophila melanogaster host larvae. These factors are localized in the female long gland and reservoir. We analyzed the protein content of these tissues and found that it strongly differed between virulent and av...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2004.10.004

    authors: Labrosse C,Stasiak K,Lesobre J,Grangeia A,Huguet E,Drezen JM,Poirie M

    更新日期:2005-02-01 00:00:00

  • Native subunit composition of two insect nicotinic receptor subtypes with differing affinities for the insecticide imidacloprid.

    abstract::Neonicotinoid insecticides, such as imidacloprid, are selective agonists of insect nicotinic acetylcholine receptors (nAChRs) and are used extensively to control a variety of insect pest species. The brown planthopper (Nilaparvata lugens), an insect pest of rice crops throughout Asia, is an important target species fo...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2009.12.003

    authors: Li J,Shao Y,Ding Z,Bao H,Liu Z,Han Z,Millar NS

    更新日期:2010-01-01 00:00:00

  • The N-glycome of the hemipteran pest insect Nilaparvata lugens reveals unexpected sex differences.

    abstract::The brown planthopper, Nilaparvata lugens, is a model species for hemimetabolous development and the most important pest insect in rice, which is the major staple crop for about half of the world population. Despite its importance, little is known of the N-glycosylation process in this insect. Here we report on the N-...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2019.01.008

    authors: Scheys F,De Schutter K,Shen Y,Yu N,Smargiasso N,De Pauw E,Van Damme EJM,Smagghe G

    更新日期:2019-04-01 00:00:00

  • Egf1.5 is a second phenoloxidase cascade inhibitor encoded by Microplitis demolitor bracovirus.

    abstract::The three-member Egf gene family from the polydnavirus Microplitis demolitor bracovirus (MdBV) encodes novel proteins distinguished by a shared cysteine-rich motif. Prior studies determined that one family member, Egf1.0, inhibits melanization of hemolymph from the moth Manduca sexta by disabling phenoloxidase activat...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2010.04.009

    authors: Lu Z,Beck MH,Strand MR

    更新日期:2010-07-01 00:00:00

  • Endogenous expression of a Bt toxin receptor in the Cry1Ac-susceptible insect cell line and its synergistic effect with cadherin on cytotoxicity of activated Cry1Ac.

    abstract::Although many insect cell lines derived from various tissues are available, it is unclear whether endogenous receptors of Bacillus thuringiensis (Bt) crystal toxins are expressed in these cell lines. In the present study, we demonstrated that the ovaries-derived Spodoptera litura Sl-HP cell line was susceptible to act...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2015.01.014

    authors: Chen Z,He F,Xiao Y,Liu C,Li J,Yang Y,Ai H,Peng J,Hong H,Liu K

    更新日期:2015-04-01 00:00:00

  • Identification of differentially expressed microRNAs in Culex pipiens and their potential roles in pyrethroid resistance.

    abstract::Pyrethroids are the major class of insecticides used for mosquito control. Excessive and improper use of insecticides, however, has resulted in pyrethroid resistance, which has become a major obstacle for mosquito control. The development of pyrethroid resistance is a complex process involving many genes, and informat...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2014.10.007

    authors: Hong S,Guo Q,Wang W,Hu S,Fang F,Lv Y,Yu J,Zou F,Lei Z,Ma K,Ma L,Zhou D,Sun Y,Zhang D,Shen B,Zhu C

    更新日期:2014-12-01 00:00:00

  • Identification of cytochrome P450 and glutathione-S-transferase genes preferentially expressed in chemosensory organs of the swallowtail butterfly, Papilio xuthus L.

    abstract::The swallowtail butterfly, Papilio xuthus L., feeds exclusively on members of the plant family, Rutaceae. Female butterflies lay eggs in response to specific chemicals contained in their host plants. They perceive a variety of polar compounds as oviposition stimulants through the tarsal chemosensilla of the foreleg by...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2005.03.013

    authors: Ono H,Ozaki K,Yoshikawa H

    更新日期:2005-08-01 00:00:00

  • Purification and properties of the soluble midgut trehalase from the gypsy moth, Lymantria dispar.

    abstract::The midgut trehalase (THA) from fifth instar Lymantria dispar (gypsy moth) larvae was purified to homogeneity by two separate methods: gel filtration followed by Rotofor preparative IEF, and affinity chromatography on trehalose coupled to Sepharose 6B followed by preparative polyacrylamide gel electrophoresis. Midgut ...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/0965-1748(93)90033-o

    authors: Valaitis AP,Bowers DF

    更新日期:1993-07-01 00:00:00

  • Early changes in the pupal transcriptome of the flesh fly Sarcophagha crassipalpis to parasitization by the ectoparasitic wasp, Nasonia vitripennis.

    abstract::We investigated changes in the pupal transcriptome of the flesh fly Sarcophaga crassipalpis, 3 and 25 h after parasitization by the ectoparasitoid wasp, Nasonia vitripennis. These time points are prior to hatching of the wasp eggs, thus the results document host responses to venom injection, rather than feeding by the...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2013.10.003

    authors: Danneels EL,Formesyn EM,Hahn DA,Denlinger DL,Cardoen D,Wenseleers T,Schoofs L,de Graaf DC

    更新日期:2013-12-01 00:00:00

  • cDNA cloning and expression of a hormone-regulated heat shock protein (hsc 70) from the prothoracic gland of Manduca sexta.

    abstract::The brain neuropeptide prothoracicotropic hormone (PTTH) stimulates a rapid increase in ecdysteroid hormone synthesis that is accompanied by general and specific increases in protein synthesis, including that of a 70 kDa cognate heat shock protein (hsc 70). To further understand the possible roles of hsc 70, hsc 70 cD...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(00)00031-x

    authors: Rybczynski R,Gilbert LI

    更新日期:2000-07-01 00:00:00

  • Biochemical changes in the transition from vitellogenesis to follicular atresia in the hematophagous Dipetalogaster maxima (Hemiptera: Reduviidae).

    abstract::In this work, we have explored the biochemical changes characterizing the transition from vitellogenesis to follicular atresia, employing the hematophagous insect vector Dipetalogaster maxima as a model. Standardized insect rearing conditions were established to induce a gradual follicular degeneration stage by depriv...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2011.06.005

    authors: Aguirre SA,Fruttero LL,Leyria J,Defferrari MS,Pinto PM,Settembrini BP,Rubiolo ER,Carlini CR,Canavoso LE

    更新日期:2011-10-01 00:00:00

  • Shifts in metabolomic profiles of the parasitoid Nasonia vitripennis associated with elevated cold tolerance induced by the parasitoid's diapause, host diapause and host diet augmented with proline.

    abstract::The ectoparasitoid wasp, Nasonia vitripennis can enhance its cold tolerance by exploiting a maternally-induced larval diapause. A simple manipulation of the fly host diapause status and supplementation of the host diet with proline also dramatically increase cold tolerance in the parasitoid. In this study, we used a m...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2015.05.012

    authors: Li Y,Zhang L,Chen H,Koštál V,Simek P,Moos M,Denlinger DL

    更新日期:2015-08-01 00:00:00

  • Identification of novel ejaculate proteins in a seed beetle and division of labour across male accessory reproductive glands.

    abstract::The male ejaculate contains a multitude of seminal fluid proteins (SFPs), many of which are key reproductive molecules, as well as sperm. However, the identification of SFPs is notoriously difficult and a detailed understanding of this complex phenotype has only been achieved in a few model species. We employed a rece...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2018.12.002

    authors: Bayram H,Sayadi A,Immonen E,Arnqvist G

    更新日期:2019-01-01 00:00:00

  • Production and characterization of monoclonal antibodies to two new mosquito Aedes aegypti salivary proteins.

    abstract::Mosquito salivary proteins, which are fundamental to the process of blood feeding, also facilitate disease transmission and cause allergic reactions. The identification and characterisation of these proteins have been hampered by the difficulty of obtaining them in purified form. In this report, we describe the produc...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(99)00066-1

    authors: Peng Z,Yang J,Wang H,Simons FE

    更新日期:1999-10-01 00:00:00

  • Biosynthesis of L-alanine, a major amino acid of fibroin in Samia cynthia ricini.

    abstract::The derivation of alanine in fibroin was investigated using NMR and selective isotopic labelling. 2H2O infused orally into 5th instar larvae was incorporated into the proton of the methyl group of alanine in fibroin. Proton exchange among alanine, glycine and serine was also found. Incorporation of 13C from [2-(13)C]a...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(99)00120-4

    authors: Osanai M,Okudaira M,Naito J,Demura M,Asakura T

    更新日期:2000-03-01 00:00:00

  • Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection.

    abstract::Targeted mutagenesis is one of the key methods for functional gene analysis. A simplified variant of gene targeting uses direct microinjection of custom-designed Zinc Finger Nuclease (ZFN) mRNAs into Drosophila embryos. To evaluate the applicability of this method to gene targeting in another insect, we mutagenized th...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2010.07.012

    authors: Takasu Y,Kobayashi I,Beumer K,Uchino K,Sezutsu H,Sajwan S,Carroll D,Tamura T,Zurovec M

    更新日期:2010-10-01 00:00:00

  • Lipid uptake by insect oocytes.

    abstract::Approximately 30-40% of the dry weight of an insect egg consists of lipid, mostly triacylglycerol (TAG). Although this lipid is essential for the energy needed by the developing embryo, little is known about the mechanism that leads to the accumulation of TAG in the insect egg. Insect oocytes can readily synthesize TA...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.ibmb.2006.01.014

    authors: Ziegler R,Van Antwerpen R

    更新日期:2006-04-01 00:00:00

  • Tissue-specific transcription of proteases and nucleases across the accessory salivary gland, principal salivary gland and gut of Nezara viridula.

    abstract::The phytophagous stink bug, Nezara viridula (L.) infests multiple plant species and impacts agricultural production worldwide. We analyzed the transcriptomes of N. viridula accessory salivary gland (ASG), principal salivary gland (PSG) and gut, with a focus on putative digestive proteases and nucleases that present a ...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2018.10.003

    authors: Liu S,Lomate PR,Bonning BC

    更新日期:2018-12-01 00:00:00

  • The ecdysone regulatory cascade and ovarian development in lepidopteran insects: insights from the silkmoth paradigm.

    abstract::The developing ovariole of the silkmoth Bombyx mori represents an excellent model for studies on the changes that occur in gene expression during the execution of long-term developmental programs. All stages of follicle development, which differ from each other by 2-2.5 h of developmental time, are organized in a sing...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.ibmb.2003.06.012

    authors: Swevers L,Iatrou K

    更新日期:2003-12-01 00:00:00

  • Low molecular weight serine protease inhibitors from insects are proteins with highly conserved sequences.

    abstract::A low molecular weight protease inhibitor peptide found in ovaries of the desert locust Schistocerca gregaria (SGPI-2), was purified from plasma of the same locust and sequenced. It was named SGCI. It was found active towards chymotrypsin and human leukocyte elastase. SGCI was synthesized using a solid-phase procedure...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(99)00109-5

    authors: Boigegrain RA,Pugnière M,Paroutaud P,Castro B,Brehélin M

    更新日期:2000-02-01 00:00:00

  • An immunophilin is a component of the insect ecdysone receptor (EcR) complex.

    abstract::The ecdysone receptor (EcR) complex has been identified in the prothoracic gland of Manduca sexta by specific immunoprecipitation and Western blot analyses, and includes EcR, ultraspiracle (USP) and FKBP46. The EcR complex binds ponasterone A in a dose-dependent manner with a Kd of 7.04 x 10(-9) M. Immunocytochemistry...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(97)00080-5

    authors: Song Q,Alnemri ES,Litwack G,Gilbert LI

    更新日期:1997-11-01 00:00:00

  • Identification and cDNA cloning of novel juvenile hormone responsive genes from fat body of the bean bug, Riptortus clavatus by mRNA differential display.

    abstract::Juvenile hormone (JH) induces termination of diapause and initiation of reproductive maturation in the adult female bean bug, Riportus clavatus. Applying PCR-based differential display, we have identified four novel JH-responsive cDNAS, that is, three repressible (JR-1, 2 and 3) and one inducible (JI-1). These DNA fra...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/s0965-1748(97)00116-1

    authors: Hirai M,Yuda M,Shinoda T,Chinzei Y

    更新日期:1998-03-01 00:00:00

  • Molybdenum cofactor deficiency causes translucent integument, male-biased lethality, and flaccid paralysis in the silkworm Bombyx mori.

    abstract::Uric acid accumulates in the epidermis of Bombyx mori larvae and renders the larval integument opaque and white. Yamamoto translucent (oya) is a novel spontaneous mutant with a translucent larval integument and unique phenotypic characteristics, such as male-biased lethality and flaccid larval paralysis. Xanthine dehy...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2016.03.008

    authors: Fujii T,Yamamoto K,Banno Y

    更新日期:2016-06-01 00:00:00

  • Characterization of voltage-sensitive sodium channel gene coding sequences from insecticide-susceptible and knockdown-resistant house fly strains.

    abstract::The kdr insecticide resistance trait of the house fly (Musca domestica .L.), which confers reduced neuronal sensitivity to DDT and pyrethroid insecticides, was previously shown to exhibit tight genetic linkage to restriction fragment length polymorphism markers lying within a voltage-sensitive sodium channel gene that...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/0965-1748(95)00093-3

    authors: Ingles PJ,Adams PM,Knipple DC,Soderlund DM

    更新日期:1996-04-01 00:00:00

  • Functional evaluation of Heat Shock Proteins 70 (HSP70/HSC70) on Rhodnius prolixus (Hemiptera, Reduviidae) physiological responses associated with feeding and starvation.

    abstract::Blood-sucking vectors must overcome thermal stress caused by intake of proportionally large amounts of warm blood from their hosts. In response to this, Heat Shock Proteins (HSPs) such as the widely studied HSP70 family (the inducible HSP70 and the cognate form HSC70, known for their role in preserving essential cellu...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章

    doi:10.1016/j.ibmb.2016.07.011

    authors: Paim RMM,Araujo RN,Leis M,Sant'anna MRV,Gontijo NF,Lazzari CR,Pereira MH

    更新日期:2016-10-01 00:00:00