N-linked glycosylation of a beetle (Apriona germari) cellulase Ag-EGase II is necessary for enzymatic activity.


:We previously reported that the beta-1,4-endoglucanase (EGase) belonging to glycoside hydrolase family (GHF) 45 of the mulberry longicorn beetle, Apriona germari (Ag-EGase II), has three potential N-linked glycosylation sites; these sites are located at amino acid residues 56-59 (NKSG), 99-102 (NSTF), and 237-239 (NYSstop). In the present study, we analyze the functional role of these potential N-linked glycosylation sites. Tunicamycin treatment completely abolished the enzymatic activity of Ag-EGase II. To further elucidate the functional role of the N-linked glycosylation sites in Ag-EGase II, we have assayed the cellulase enzyme activity in Ser58Gln, Thr101Gln, or Ser239Gln mutants. Lack of N-linked glycosylation site at residues 99-102 (NSTF), the site of which is conserved in known beetle GHF 45 cellulases, showed loss of enzyme activity and reduced the molecular mass of the enzyme. In contrast, mutations in Ser58Gln or Ser239Gln affected neither the activity nor the apparent molecular mass of the enzyme, indicating that these sites did not lead to N-linked glycosylation. The present study demonstrates that N-linked glycosylation at residues 99-102 (NSTF), while not essential for secretion, is required for Ag-EGase II enzyme activity.


Wei YD,Lee KS,Gui ZZ,Yoon HJ,Kim I,Je YH,Lee SM,Zhang GZ,Guo X,Sohn HD,Jin BR




Has Abstract


2006-06-01 00:00:00














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    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Kimoto M,Tsubota T,Uchino K,Sezutsu H,Takiya S

    更新日期:2015-01-01 00:00:00

  • Considerations on the structural evidence of a ligand-binding function of ultraspiracle, an insect homolog of vertebrate RXR.

    abstract::This analysis considers the structural evidence of a ligand-binding function of the nuclear receptor ultraspiracle (USP). The positions and nature of residues in the ligand-binding domain of USP from six higher insects is evaluated in comparison to the function of conserved residues vertebrate receptors that have been...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章,评审


    authors: Jones G,Jones D

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    pub_type: 杂志文章


    authors: Cui F,Lin Z,Wang H,Liu S,Chang H,Reeck G,Qiao C,Raymond M,Kang L

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  • Silencing the odorant receptor co-receptor RproOrco affects the physiology and behavior of the Chagas disease vector Rhodnius prolixus.

    abstract::Olfaction is one of the main sensory modalities that allow insects to interpret their environment. Several proteins, including odorant-binding proteins (OBPs) and odorant receptors (ORs), are involved in this process. Odorant receptors are ion channels formed by a binding unit OR and an odorant receptor co-receptor (O...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Franco TA,Oliveira DS,Moreira MF,Leal WS,Melo AC

    更新日期:2016-02-01 00:00:00

  • Insect natural products and processes: new treatments for human disease.

    abstract::In this overview, some of the more significant recent developments in bioengineering natural products from insects with use or potential use in modern medicine are described, as well as in utilisation of insects as models for studying essential mammalian processes such as immune responses to pathogens. To date, insect...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章,评审


    authors: Ratcliffe NA,Mello CB,Garcia ES,Butt TM,Azambuja P

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  • A RhoGAP protein as a main immune suppressive factor in the Leptopilina boulardi (Hymenoptera, Figitidae)-Drosophila melanogaster interaction.

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    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Labrosse C,Stasiak K,Lesobre J,Grangeia A,Huguet E,Drezen JM,Poirie M

    更新日期:2005-02-01 00:00:00

  • Native subunit composition of two insect nicotinic receptor subtypes with differing affinities for the insecticide imidacloprid.

    abstract::Neonicotinoid insecticides, such as imidacloprid, are selective agonists of insect nicotinic acetylcholine receptors (nAChRs) and are used extensively to control a variety of insect pest species. The brown planthopper (Nilaparvata lugens), an insect pest of rice crops throughout Asia, is an important target species fo...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Li J,Shao Y,Ding Z,Bao H,Liu Z,Han Z,Millar NS

    更新日期:2010-01-01 00:00:00

  • The N-glycome of the hemipteran pest insect Nilaparvata lugens reveals unexpected sex differences.

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    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Scheys F,De Schutter K,Shen Y,Yu N,Smargiasso N,De Pauw E,Van Damme EJM,Smagghe G

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  • Egf1.5 is a second phenoloxidase cascade inhibitor encoded by Microplitis demolitor bracovirus.

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    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Lu Z,Beck MH,Strand MR

    更新日期:2010-07-01 00:00:00

  • Endogenous expression of a Bt toxin receptor in the Cry1Ac-susceptible insect cell line and its synergistic effect with cadherin on cytotoxicity of activated Cry1Ac.

    abstract::Although many insect cell lines derived from various tissues are available, it is unclear whether endogenous receptors of Bacillus thuringiensis (Bt) crystal toxins are expressed in these cell lines. In the present study, we demonstrated that the ovaries-derived Spodoptera litura Sl-HP cell line was susceptible to act...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Chen Z,He F,Xiao Y,Liu C,Li J,Yang Y,Ai H,Peng J,Hong H,Liu K

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  • Identification of differentially expressed microRNAs in Culex pipiens and their potential roles in pyrethroid resistance.

    abstract::Pyrethroids are the major class of insecticides used for mosquito control. Excessive and improper use of insecticides, however, has resulted in pyrethroid resistance, which has become a major obstacle for mosquito control. The development of pyrethroid resistance is a complex process involving many genes, and informat...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Hong S,Guo Q,Wang W,Hu S,Fang F,Lv Y,Yu J,Zou F,Lei Z,Ma K,Ma L,Zhou D,Sun Y,Zhang D,Shen B,Zhu C

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  • Identification of cytochrome P450 and glutathione-S-transferase genes preferentially expressed in chemosensory organs of the swallowtail butterfly, Papilio xuthus L.

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    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Ono H,Ozaki K,Yoshikawa H

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  • Purification and properties of the soluble midgut trehalase from the gypsy moth, Lymantria dispar.

    abstract::The midgut trehalase (THA) from fifth instar Lymantria dispar (gypsy moth) larvae was purified to homogeneity by two separate methods: gel filtration followed by Rotofor preparative IEF, and affinity chromatography on trehalose coupled to Sepharose 6B followed by preparative polyacrylamide gel electrophoresis. Midgut ...

    journal_title:Insect biochemistry and molecular biology

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    journal_title:Insect biochemistry and molecular biology

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    authors: Danneels EL,Formesyn EM,Hahn DA,Denlinger DL,Cardoen D,Wenseleers T,Schoofs L,de Graaf DC

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    authors: Rybczynski R,Gilbert LI

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    journal_title:Insect biochemistry and molecular biology

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    authors: Aguirre SA,Fruttero LL,Leyria J,Defferrari MS,Pinto PM,Settembrini BP,Rubiolo ER,Carlini CR,Canavoso LE

    更新日期:2011-10-01 00:00:00

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    abstract::The ectoparasitoid wasp, Nasonia vitripennis can enhance its cold tolerance by exploiting a maternally-induced larval diapause. A simple manipulation of the fly host diapause status and supplementation of the host diet with proline also dramatically increase cold tolerance in the parasitoid. In this study, we used a m...

    journal_title:Insect biochemistry and molecular biology

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    authors: Li Y,Zhang L,Chen H,Koštál V,Simek P,Moos M,Denlinger DL

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  • Identification of novel ejaculate proteins in a seed beetle and division of labour across male accessory reproductive glands.

    abstract::The male ejaculate contains a multitude of seminal fluid proteins (SFPs), many of which are key reproductive molecules, as well as sperm. However, the identification of SFPs is notoriously difficult and a detailed understanding of this complex phenotype has only been achieved in a few model species. We employed a rece...

    journal_title:Insect biochemistry and molecular biology

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    authors: Bayram H,Sayadi A,Immonen E,Arnqvist G

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  • Production and characterization of monoclonal antibodies to two new mosquito Aedes aegypti salivary proteins.

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    journal_title:Insect biochemistry and molecular biology

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    authors: Peng Z,Yang J,Wang H,Simons FE

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    journal_title:Insect biochemistry and molecular biology

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    authors: Osanai M,Okudaira M,Naito J,Demura M,Asakura T

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  • Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection.

    abstract::Targeted mutagenesis is one of the key methods for functional gene analysis. A simplified variant of gene targeting uses direct microinjection of custom-designed Zinc Finger Nuclease (ZFN) mRNAs into Drosophila embryos. To evaluate the applicability of this method to gene targeting in another insect, we mutagenized th...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Takasu Y,Kobayashi I,Beumer K,Uchino K,Sezutsu H,Sajwan S,Carroll D,Tamura T,Zurovec M

    更新日期:2010-10-01 00:00:00

  • Lipid uptake by insect oocytes.

    abstract::Approximately 30-40% of the dry weight of an insect egg consists of lipid, mostly triacylglycerol (TAG). Although this lipid is essential for the energy needed by the developing embryo, little is known about the mechanism that leads to the accumulation of TAG in the insect egg. Insect oocytes can readily synthesize TA...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章,评审


    authors: Ziegler R,Van Antwerpen R

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  • Tissue-specific transcription of proteases and nucleases across the accessory salivary gland, principal salivary gland and gut of Nezara viridula.

    abstract::The phytophagous stink bug, Nezara viridula (L.) infests multiple plant species and impacts agricultural production worldwide. We analyzed the transcriptomes of N. viridula accessory salivary gland (ASG), principal salivary gland (PSG) and gut, with a focus on putative digestive proteases and nucleases that present a ...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Liu S,Lomate PR,Bonning BC

    更新日期:2018-12-01 00:00:00

  • The ecdysone regulatory cascade and ovarian development in lepidopteran insects: insights from the silkmoth paradigm.

    abstract::The developing ovariole of the silkmoth Bombyx mori represents an excellent model for studies on the changes that occur in gene expression during the execution of long-term developmental programs. All stages of follicle development, which differ from each other by 2-2.5 h of developmental time, are organized in a sing...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章,评审


    authors: Swevers L,Iatrou K

    更新日期:2003-12-01 00:00:00

  • Low molecular weight serine protease inhibitors from insects are proteins with highly conserved sequences.

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    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Boigegrain RA,Pugnière M,Paroutaud P,Castro B,Brehélin M

    更新日期:2000-02-01 00:00:00

  • An immunophilin is a component of the insect ecdysone receptor (EcR) complex.

    abstract::The ecdysone receptor (EcR) complex has been identified in the prothoracic gland of Manduca sexta by specific immunoprecipitation and Western blot analyses, and includes EcR, ultraspiracle (USP) and FKBP46. The EcR complex binds ponasterone A in a dose-dependent manner with a Kd of 7.04 x 10(-9) M. Immunocytochemistry...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Song Q,Alnemri ES,Litwack G,Gilbert LI

    更新日期:1997-11-01 00:00:00

  • Identification and cDNA cloning of novel juvenile hormone responsive genes from fat body of the bean bug, Riptortus clavatus by mRNA differential display.

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    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Hirai M,Yuda M,Shinoda T,Chinzei Y

    更新日期:1998-03-01 00:00:00

  • Molybdenum cofactor deficiency causes translucent integument, male-biased lethality, and flaccid paralysis in the silkworm Bombyx mori.

    abstract::Uric acid accumulates in the epidermis of Bombyx mori larvae and renders the larval integument opaque and white. Yamamoto translucent (oya) is a novel spontaneous mutant with a translucent larval integument and unique phenotypic characteristics, such as male-biased lethality and flaccid larval paralysis. Xanthine dehy...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Fujii T,Yamamoto K,Banno Y

    更新日期:2016-06-01 00:00:00

  • Characterization of voltage-sensitive sodium channel gene coding sequences from insecticide-susceptible and knockdown-resistant house fly strains.

    abstract::The kdr insecticide resistance trait of the house fly (Musca domestica .L.), which confers reduced neuronal sensitivity to DDT and pyrethroid insecticides, was previously shown to exhibit tight genetic linkage to restriction fragment length polymorphism markers lying within a voltage-sensitive sodium channel gene that...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Ingles PJ,Adams PM,Knipple DC,Soderlund DM

    更新日期:1996-04-01 00:00:00

  • Functional evaluation of Heat Shock Proteins 70 (HSP70/HSC70) on Rhodnius prolixus (Hemiptera, Reduviidae) physiological responses associated with feeding and starvation.

    abstract::Blood-sucking vectors must overcome thermal stress caused by intake of proportionally large amounts of warm blood from their hosts. In response to this, Heat Shock Proteins (HSPs) such as the widely studied HSP70 family (the inducible HSP70 and the cognate form HSC70, known for their role in preserving essential cellu...

    journal_title:Insect biochemistry and molecular biology

    pub_type: 杂志文章


    authors: Paim RMM,Araujo RN,Leis M,Sant'anna MRV,Gontijo NF,Lazzari CR,Pereira MH

    更新日期:2016-10-01 00:00:00