Abstract:
:Bombyx mori, the domesticated silkworm, is a major insect model for research, and the first lepidopteran for which draft genome sequences became available in 2004. Two independent data sets from whole-genome shotgun sequencing were merged and assembled together with newly obtained fosmid- and BAC-end sequences. The remarkably improved new assembly is presented here. The 8.5-fold sequence coverage of an estimated 432 Mb genome was assembled into scaffolds with an N50 size of approximately 3.7 Mb; the largest scaffold was 14.5 million base pairs. With help of a high-density SNP linkage map, we anchored 87% of the scaffold sequences to all 28 chromosomes. A particular feature was the high repetitive sequence content estimated to be 43.6% and that consisted mainly of transposable elements. We predicted 14,623 gene models based on a GLEAN-based algorithm, a more accurate prediction than the previous gene models for this species. Over three thousand silkworm genes have no homologs in other insect or vertebrate genomes. Some insights into gene evolution and into characteristic biological processes are presented here and in other papers in this issue. The massive silk production correlates with the existence of specific tRNA clusters, and of several sericin genes assembled in a cluster. The silkworm's adaptation to feeding on mulberry leaves, which contain toxic alkaloids, is likely linked to the presence of new-type sucrase genes, apparently acquired from bacteria. The silkworm genome also revealed the cascade of genes involved in the juvenile hormone biosynthesis pathway, and a large number of cuticular protein genes.
journal_name
Insect Biochem Mol Bioljournal_title
Insect biochemistry and molecular biologyauthors
International Silkworm Genome Consortium.doi
10.1016/j.ibmb.2008.11.004subject
Has Abstractpub_date
2008-12-01 00:00:00pages
1036-45issue
12eissn
0965-1748issn
1879-0240pii
S0965-1748(08)00209-9journal_volume
38pub_type
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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abstract::The present paper describes the effect of juvenile hormone III (JH III) upon vitellogenin (Vg) gene expression in cardioallatectomized females of Blattella germanica. Northern blot analyses of time course studies showed that Vg mRNA can be detected 2 h after the treatment with 1 microgram of JH III. Western blot analy...
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1999-11-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1016/j.ibmb.2020.103392
更新日期:2020-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0965-1748(01)00145-x
更新日期:2002-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.ibmb.2012.06.001
更新日期:2012-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0965-1748(01)00040-6
更新日期:2001-09-01 00:00:00
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(98)00121-0
更新日期:1999-02-01 00:00:00
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/s0965-1748(00)00090-4
更新日期:2000-12-01 00:00:00
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
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更新日期:2013-12-01 00:00:00
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journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
doi:10.1016/j.ibmb.2004.10.004
更新日期:2005-02-01 00:00:00
abstract::To understand the role that 20-hydroxy-ecdysone (20E) plays during ovarian development in Bombyx mori, we have undertaken the cloning of the silkworm ecdysone receptor (EcR) and a study of its expression during follicular cell differentiation. We have cloned a cDNA that contains a complete open reading frame for a 68....
journal_title:Insect biochemistry and molecular biology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1016/j.ibmb.2011.03.004
更新日期:2011-08-01 00:00:00