Early activation, motility, and homing of neonatal microglia to injured neurons does not require protein synthesis.

Abstract:

:Neuronal injury in CNS tissues induces a rapid activation and mobilization of resident microglia (MG). It is widely assumed that changes in gene expression drive the morphological transformation of MG and regulate their mobilization during activation. Here, we used acutely excised neonatal rat brain slices to test whether the morphological transformation and homing of MG to injured neurons requires gene expression and de novo protein synthesis. Traumatic injury during excision of live brain tissue slices induces a rapid and transient translocation of a transcription factor, NF-kappaB, to nuclei in MG. This is followed within 4-8 h by an increase in immunolabeling for cell adhesion molecules and lysosomal proteins, accompanied by changes in cell morphology. Application of anisomycin, a protein synthesis inhibitor, prevents the increase in immunolabeling for markers of MG activation but not the morphological transformation. Confocal time-lapse imaging in live tissue slices indicates that MG cell motility (branch extension and retraction) and locomotion are unaffected by anisomycin at early postinjury time-points (<4 h), while at later time-points (4-8 h postinjury) MG locomotion but not motility is inhibited. Thus, activated MG rapidly localize to injured pyramidal neuron cell bodies by 4-h postinjury, even in the presence of anisomycin. Moreover, this early MG activation and homing to injured neurons is unaffected in tissue slices from beta2 integrin deficient mice. These results indicate that gene activation and new protein synthesis coincide with, but are not necessary for, the rapid morphological transformation and early migration-dependent homing of activated MG to injured neurons in CNS tissues.

journal_name

Glia

journal_title

Glia

authors

Kurpius D,Wilson N,Fuller L,Hoffman A,Dailey ME

doi

10.1002/glia.20355

subject

Has Abstract

pub_date

2006-07-01 00:00:00

pages

58-70

issue

1

eissn

0894-1491

issn

1098-1136

journal_volume

54

pub_type

杂志文章

相关文献

GLIA文献大全
  • Atrial natriuretic peptide-like (ANP-LIR) and ANP prohormone immunoreactive astrocytes and neurons of human cerebral cortex.

    abstract::Atrial natriuretic peptide (ANP) represents a family of related peptides originally isolated from cardiac atria that have potent natriuretic, diuretic, and vasorelaxant properties. ANP has previously been localized in neurons of the rat brain in regions subserving cardiovascular functions and fluid/electrolyte balance...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440120308

    authors: McKenzie JC,Berman NE,Thomas CR,Young JK,Compton LY,Cothran LN,Liu WL,Klein RM

    更新日期:1994-11-01 00:00:00

  • Tripchlorolide protects neuronal cells from microglia-mediated beta-amyloid neurotoxicity through inhibiting NF-kappaB and JNK signaling.

    abstract::Recent research has focused on soluble oligomeric assemblies of beta-amyloid peptides (Abeta) as the proximate cause of neuroinflammation, synaptic loss, and the eventual dementia associated with Alzheimer's disease (AD). In this study, tripchlorolide (T4), an extract of Tripterygium wilfordii Hook. F (TWHF), was stud...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20844

    authors: Pan XD,Chen XC,Zhu YG,Chen LM,Zhang J,Huang TW,Ye QY,Huang HP

    更新日期:2009-08-15 00:00:00

  • Heterogeneous fate choice of genetically modulated adult neural stem cells in gray and white matter of the central nervous system.

    abstract::Apart from dedicated oligodendroglial progenitor cells, adult neural stem cells (aNSCs) can also give rise to new oligodendrocytes in the adult central nervous system (CNS). This process mainly confers myelinating glial cell replacement in pathological situations and can hence contribute to glial heterogeneity. Our pr...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.23724

    authors: Beyer F,Jadasz J,Samper Agrelo I,Schira-Heinen J,Groh J,Manousi A,Bütermann C,Estrada V,Reiche L,Cantone M,Vera J,Viganò F,Dimou L,Müller HW,Hartung HP,Küry P

    更新日期:2020-02-01 00:00:00

  • Calcium-mobilizing and electrophysiological effects of bradykinin on cortical astrocyte subtypes in culture.

    abstract::The possible consequences of activating bradykinin (Bk) receptors on identified astrocyte subtypes derived from rat cortex have been investigated in terms of: 1) mobilization of intracellular Ca2+; and 2) electrophysiological response. Bk induced a rapid, transient rise in intracellular Ca2+ in 63% of cortical type-1-...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440090405

    authors: Stephens GJ,Cholewinski AJ,Wilkin GP,Djamgoz MB

    更新日期:1993-12-01 00:00:00

  • Differential cell proliferation in the cortex of the APPswePS1dE9 Alzheimer's disease mouse model.

    abstract::Plaque deposition in Alzheimer's disease (AD) is known to decrease proliferation in neurogenic niches in AD mouse models, but the effects on cell proliferation and differentiation in other brain areas have not been studied in detail. We analyzed cell proliferation in the cortex of wild type (WT) and APPswePS1dE9 trans...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.22295

    authors: Kamphuis W,Orre M,Kooijman L,Dahmen M,Hol EM

    更新日期:2012-04-01 00:00:00

  • Effect of benzodiazepines and pentobarbital on the GABA-induced depolarization in cultured astrocytes.

    abstract::We have previously shown that cultured astrocytes from neonatal rat cerebral cortex are depolarized by GABA. The underlying ionic mechanism, activation of a Cl- conductance and responses to an agonist and antagonists were found to be similar to those of the neuronal GABAA receptor (Kettenmann et al.: Brain Research 40...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440010205

    authors: Backus KH,Kettenmann H,Schachner M

    更新日期:1988-01-01 00:00:00

  • Membrane-initiated effects of progesterone on calcium dependent signaling and activation of VEGF gene expression in retinal glial cells.

    abstract::Neurosteroids, such as progesterone, influence central nervous system development and function by regulating a broad spectrum of physiological processes. Here, we investigated membrane-initiated actions of progesterone in the retina and identified the membrane-associated progesterone receptor component 1 (PGRMC1). We ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20523

    authors: Swiatek-De Lange M,Stampfl A,Hauck SM,Zischka H,Gloeckner CJ,Deeg CA,Ueffing M

    更新日期:2007-08-01 00:00:00

  • Myelination, oligodendrocytes, and serious mental illness.

    abstract::Historically, the human brain has been conceptually segregated from the periphery and further dichotomized into gray matter (GM) and white matter (WM) based on the whitish appearance of the exceptionally high lipid content of the myelin sheaths encasing neuronal axons. These simplistic dichotomies were unfortunately e...

    journal_title:Glia

    pub_type: 杂志文章,评审

    doi:10.1002/glia.22716

    authors: Haroutunian V,Katsel P,Roussos P,Davis KL,Altshuler LL,Bartzokis G

    更新日期:2014-11-01 00:00:00

  • Patterned distribution of immunoreactive astroglial processes in the striate (V1) cortex of New World monkeys.

    abstract::The possibility of a modular organization of non-neuronal elements was analyzed in the opercular region of the striate neocortex in adult New World monkeys. For this purpose, and in order to follow possible correlations in the general organization of neuronal and astroglial elements, immunocytochemical procedures for ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:

    authors: Colombo JA,Schleicher A,Zilles K

    更新日期:1999-01-01 00:00:00

  • Selectively increased expression of the astrocytic/endothelial glucose transporter protein GLUT1 in acute liver failure.

    abstract::Acute liver failure (ALF) is consistently accompanied by alterations in brain energy metabolites and recent nuclear magnetic resonance (NMR) studies suggest disturbances in brain oxidative metabolism in experimental ALF. Glucose transport across the blood-brain barrier is essential to sustain brain energy metabolism a...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20310

    authors: Bélanger M,Desjardins P,Chatauret N,Butterworth RF

    更新日期:2006-04-01 00:00:00

  • Activation of dorsal horn microglia contributes to diabetes-induced tactile allodynia via extracellular signal-regulated protein kinase signaling.

    abstract::Painful neuropathy is one of the most common complications of diabetes, one hallmark of which is tactile allodynia (pain hypersensitivity to innocuous stimulation). The underlying mechanisms of tactile allodynia are, however, poorly understood. Emerging evidence indicates that, following nerve injury, activated microg...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20623

    authors: Tsuda M,Ueno H,Kataoka A,Tozaki-Saitoh H,Inoue K

    更新日期:2008-03-01 00:00:00

  • H-ferritin is the major source of iron for oligodendrocytes.

    abstract::There is a critical relationship between oligodendrocyte development, myelin production, and iron bioavailability. Iron deficiency leads to hypomyelination both in humans and animal models, and the neurological sequelae of hypomyelination are significant. Therefore, understanding molecular mechanisms of iron import in...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.21164

    authors: Todorich B,Zhang X,Connor JR

    更新日期:2011-06-01 00:00:00

  • GSK3β regulates oligodendrogenesis in the dorsal microdomain of the subventricular zone via Wnt-β-catenin signaling.

    abstract::Oligodendrocytes, the myelinating cells of the CNS, are derived postnatally from oligodendrocyte precursors (OPs) of the subventricular zone (SVZ). However, the mechanisms that regulate their generation from SVZ neural stem cells (NSC) are poorly understood. Here, we have examined the role of glycogen synthase kinase ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.22641

    authors: Azim K,Rivera A,Raineteau O,Butt AM

    更新日期:2014-05-01 00:00:00

  • Disruption of transient receptor potential canonical channel 1 causes incomplete cytokinesis and slows the growth of human malignant gliomas.

    abstract::Despite decades of research, primary brain tumors, gliomas, lack effective treatment options and present a huge clinical challenge. Particularly, the most malignant subtype, Glioblastoma multiforme, proliferates extensively and cells often undergo incomplete cell divisions, resulting in multinucleated cells. We now pr...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20994

    authors: Bomben VC,Sontheimer H

    更新日期:2010-08-01 00:00:00

  • Schwann cell heparan sulfate proteoglycans play a critical role in glial growth factor/neuregulin signaling.

    abstract::Glial growth factors are proteins encoded by the neuregulin gene and are thought to signal via receptor tyrosine kinases. Many neuregulin gene products bind heparin, and we hypothesize that affinity for heparin may implicate cell surface heparan sulfate proteoglycans (HeSPGs) as co-receptors for the soluble neuregulin...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-1136(199605)17:1<28::AID-GLIA3>

    authors: Sudhalter J,Whitehouse L,Rusche JR,Marchionni MA,Mahanthappa NK

    更新日期:1996-05-01 00:00:00

  • Bradykinin induces inositol 1,4,5-trisphosphate-dependent hyperpolarization in K+ M-current-deficient hybrid NL308 cells: comparison with NG108-15 neuroblastoma x glioma hybrid cells.

    abstract::External application of bradykinin (BK) to mouse neuroblastoma X mouse fibroblast hybrid NL308 cells and mouse neuroblastoma X rat glioma hybrid NG108-15 cells produced a transient outward (hyperpolarizing) current. In NG108-15 cells, BK also induced an inward (depolarizing) current associated with a decrease in input...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440030102

    authors: Higashida H,Okano Y,Hoshi N,Yada Y,Yokoyama S,Asaga T,Fu T,Nozawa Y

    更新日期:1990-01-01 00:00:00

  • Astrocytic poly(ADP-ribose) polymerase-1 activation leads to bioenergetic depletion and inhibition of glutamate uptake capacity.

    abstract::Poly(ADP-ribose) polymerase-1 (PARP-1) is a ubiquitous nuclear enzyme involved in genomic stability. Excessive oxidative DNA strand breaks lead to PARP-1-induced depletion of cellular NAD(+), glycolytic rate, ATP levels, and eventual cell death. Glutamate neurotransmission is tightly controlled by ATP-dependent astroc...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.20936

    authors: Tang KS,Suh SW,Alano CC,Shao Z,Hunt WT,Swanson RA,Anderson CM

    更新日期:2010-03-01 00:00:00

  • The nutritive function of glia is regulated by signals released by neurons.

    abstract::The idea of a metabolic coupling between neurons and astrocytes in the brain has been entertained for about 100 years. The use recently of simple and well-compartmentalized nervous systems, such as the honeybee retina or purified preparations of neurons and glia, provided strong support for a nutritive function of gli...

    journal_title:Glia

    pub_type: 杂志文章

    doi:

    authors: Tsacopoulos M,Poitry-Yamate CL,Poitry S,Perrottet P,Veuthey AL

    更新日期:1997-09-01 00:00:00

  • Laminin molecules in freeze-treated nerve segments are associated with migrating Schwann cells that display the corresponding alpha6beta1 integrin receptor.

    abstract::Isolated acellular nerve segments protected from migration of Schwann cells and the acellular nerve segments joined with the distal nerve stumps were prepared by a repeated freeze-thaw procedure in the rat sciatic nerves. The presence of laminin-1 and -2, as well as alpha6 and beta1 integrin chains, was detected by in...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/1098-1136(20010101)33:1<36::aid-glia1004>3

    authors: Dubový P,Svízenská I,Klusáková I,Zítková A,Houst'Ava L,Haninec P

    更新日期:2001-01-01 00:00:00

  • Myelin/oligodendrocyte glycoprotein (MOG) expression is associated with myelin deposition.

    abstract::We investigated the onset of expression of the myelin/oligodendrocyte glycoprotein (MOG) mRNA and protein in the developing mouse central nervous system. In situ hybridization on brain sections at different stages of embryonic and postnatal development showed that MOG transcripts were first detected at birth in the me...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-1136(199609)18:1<39::AID-GLIA4>

    authors: Solly SK,Thomas JL,Monge M,Demerens C,Lubetzki C,Gardinier MV,Matthieu JM,Zalc B

    更新日期:1996-09-01 00:00:00

  • Novel microtubule-associated protein-2 isoform is expressed early in human oligodendrocyte maturation.

    abstract::We have identified a developmentally regulated, oligodendrocyte-specific protein, designated microtubule-associated protein-2 expressing exon 13 (MAP-2+13), in the human central nervous system (CNS). Monoclonal antibodies directed against MAP-2+13 labeled oligodendrocytes in the white matter of human fetal spinal cord...

    journal_title:Glia

    pub_type: 杂志文章

    doi:

    authors: Shafit-Zagardo B,Davies P,Rockwood J,Kress Y,Lee SC

    更新日期:2000-02-01 00:00:00

  • The mechanistic target of rapamycin pathway downregulates bone morphogenetic protein signaling to promote oligodendrocyte differentiation.

    abstract::Oligodendrocyte precursor cells (OPCs) differentiate and mature into oligodendrocytes, which produce myelin in the central nervous system. Prior studies have shown that the mechanistic target of rapamycin (mTOR) is necessary for proper myelination of the mouse spinal cord and that bone morphogenetic protein (BMP) sign...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.23776

    authors: Ornelas IM,Khandker L,Wahl SE,Hashimoto H,Macklin WB,Wood TL

    更新日期:2020-06-01 00:00:00

  • MiR-409-3p and MiR-1896 co-operatively participate in IL-17-induced inflammatory cytokine production in astrocytes and pathogenesis of EAE mice via targeting SOCS3/STAT3 signaling.

    abstract::Th17 cells and interleukin-17 (IL-17) have been found to play an important role in the pathology of multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE). Response to IL-17, reactive astrocytes accompany with immune cells infiltration and axonal damage in MS/EAE. However, the ro...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.23530

    authors: Liu X,Zhou F,Yang Y,Wang W,Niu L,Zuo D,Li X,Hua H,Zhang B,Kou Y,Guo J,Kong F,Pan W,Gao D,Meves JM,Sun H,Xue M,Zhang Q,Wang Y,Tang R

    更新日期:2019-01-01 00:00:00

  • Chronically denervated rat Schwann cells respond to GGF in vitro.

    abstract::C-erbB receptor/neuregulin signalling plays a significant role in Schwann cell function. In vivo, Schwann cells up-regulate expression of c-erbB receptors in the first month after injury, but receptor expression is down-regulated with time to levels that are not detectable immunohistochemically. The inability of chron...

    journal_title:Glia

    pub_type: 杂志文章

    doi:

    authors: Li H,Wigley C,Hall SM

    更新日期:1998-11-01 00:00:00

  • Calcium waves between astrocytes from Cx43 knockout mice.

    abstract::Gap junctions are regarded as the primary pathway underlying propagation of Ca2+ waves between astrocytes, although signaling through extracellular space may also contribute. Results obtained from astrocytes cultured from sibling Cx43 knockout (KO) and wild-type (WT) mice in six litters showed that Ca2+ waves propagat...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-1136(199809)24:1<65::aid-glia7>

    authors: Scemes E,Dermietzel R,Spray DC

    更新日期:1998-09-01 00:00:00

  • Denervated skeletal muscle stimulates migration of Schwann cells from the distal stump of transected peripheral nerve: an in vivo study.

    abstract::We have tested the stimulation of Schwann cell migration from the distal stump of a 1 week transected sciatic nerve of adult rats by denervated skeletal muscle. Migrating Schwann cells were distinguished by the presence of non-specific cholinesterase (nChE) activity and glial fibrillary acidic protein (GFAP) at a dist...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440120203

    authors: Dubový P,Svízenská I

    更新日期:1994-10-01 00:00:00

  • Endothelin-1 increases 2-arachidonoyl glycerol (2-AG) production in astrocytes.

    abstract::Astrocytes play an important role in neuroprotective responses. Recent studies indicate that endothelin-1, a neuropeptide upregulated during brain injury, increases levels of the endocannabinoid anandamide, a lipid with neuroprotective properties, in astrocytes in primary cultures. However, whether this neuropeptide a...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.10270

    authors: Walter L,Stella N

    更新日期:2003-10-01 00:00:00

  • Media that support the growth and differentiation of oligodendrocytes do not induce olfactory ensheathing cells to express a myelinating phenotype.

    abstract::The glial cells that ensheath olfactory axons are referred to as ensheathing cells. In vivo, these non-myelinating glial cells express a mixture of astrocyte-specific and Schwann cell-specific phenotypic features with the former cellular phenotype predominating, but in vitro can assemble a myelin sheath when co-cultur...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440100408

    authors: Doucette R,Devon R

    更新日期:1994-04-01 00:00:00

  • S100B expression in and effects on microglia.

    abstract::We evaluated the intracellular and extracellular biological role of S100B protein with respect to microglia. S100B, which belongs to the multigenic family of Ca2+-binding proteins, is abundant in astrocytes where it is found diffusely in the cytoplasm and is associated with membranes and cytoskeleton constituents. S10...

    journal_title:Glia

    pub_type: 杂志文章

    doi:

    authors: Adami C,Sorci G,Blasi E,Agneletti AL,Bistoni F,Donato R

    更新日期:2001-02-01 00:00:00

  • Migration of Schwann cells from the distal stump of the sciatic nerve 1 week after transection: the effects of insulin and cytosine arabinoside.

    abstract::We have examined the migratory capacity of Schwann cells from the distal stump of a 1-week transected sciatic nerve of adult rat for a distance of 10 mm. The distal stump was introduced into the open end of a silicone chamber packed with artificial fibrin sponge (Gelaspon) soaked in phosphate-buffered saline (control ...

    journal_title:Glia

    pub_type: 杂志文章

    doi:10.1002/glia.440060406

    authors: Dubový P,Svízenská I

    更新日期:1992-01-01 00:00:00