Abstract:
:Human brain macrophages (microglia) have been isolated from mixed brain cell cultures initiated from explants of neurosurgical adult human tissue in one step according to a method developed for rat microglia. Cells were characterized enzyme-histochemically (NDPase) in mixed and immunocytochemically (anti-CD 14) in mixed and isolated cultures. Purified cells were used to investigate in more detail membrane currents by the patch clamp technique. In 14 cells microdialyzed with a standard, K(+)-containing intracellular solution there was no indication for a hyperpolarization-induced K(+)-inward current characteristic for newborn rat microglia. However, in 12 cells depolarizing pulses initiated a rapidly inactivating inward current which was followed by an outward current (in 4 cells). The outward current appeared to be carried by K+, since it was absent in another 18 cells, recorded by micropipettes containing Cs+ instead of K+ as the main intracellular cation. The depolarization-induced inward current persisted under these conditions. This current was inhibited by tetrodotoxin (5 microM) and by substitution of Na+ by choline in the bath solution. It is suggested that this Na(+)-current is specifically expressed in macrophages derived from adult brain.
journal_name
Gliajournal_title
Gliaauthors
Nörenberg W,Illes P,Gebicke-Haerter PJdoi
10.1002/glia.440100303subject
Has Abstractpub_date
1994-03-01 00:00:00pages
165-72issue
3eissn
0894-1491issn
1098-1136journal_volume
10pub_type
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