The use of newly developed real-time PCR for the rapid identification of bacteria in culture-negative osteomyelitis.

Abstract:

:We report a case of a culture-negative osteomyelitis in which our newly developed real-time polymerase chain reaction (PCR) could differentiate Staphylococcus aureus from Staphylococcus epidermidis. This is the first report that described the application of this novel assay to an orthopedics clinical sample. This assay may be useful for other clinical culture-negative cases in a combination with a broad-spectrum assay as a rapid microorganism identification method.

journal_name

Joint Bone Spine

journal_title

Joint bone spine

authors

Kobayashi N,Bauer TW,Sakai H,Togawa D,Lieberman IH,Fujishiro T,Procop GW

doi

10.1016/j.jbspin.2005.11.014

subject

Has Abstract

pub_date

2006-12-01 00:00:00

pages

745-7

issue

6

eissn

1297-319X

issn

1778-7254

pii

S1297-319X(06)00093-5

journal_volume

73

pub_type

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