Abstract：
OBJECTIVE:We have shown that buccal epithelial cells (BEC) from humans can contain a polymicrobial intracellular flora. Members of that flora can induce proinflammatory responses. However, our subjects all had healthy oral mucosa. This might reflect tolerance of bacterial invasion by live BEC. Alternatively, inflammation might not occur if invaded cells were mostly dead, and thus unable to mount a response. This study addressed that issue, by determining the vital status of BEC and the bacteria associated with them. DESIGN:Initial experiments indicated that BEC were anomalously permeable to the DNA stain propidium iodide. We used that property to develop a protocol that combined the DNA stains SYTO 9 and propidium iodide (indicators of bacterial viability) with the esterase substrate calcein blue AM (an indicator of BEC viability), and Annexin V Alexa Fluor 647 conjugate (an apoptosis marker). That protocol was applied to BEC collected from 36 human subjects. RESULTS:On average, 70% of BEC displayed calcein blue staining, with no binding of Annexin V, 25% showed signs of apoptosis, and 5% did not stain with calcein blue. The mean percent of BEC with live cell-associated bacteria was 29%. Collectively, 25% of total BEC displayed calcein blue staining and live (SYTO 9 stained) bacteria. Only 1% of total BEC were negative for calcein blue and associated with live bacteria. CONCLUSIONS:Our findings suggest that live BEC are tolerant of bacterial invasion. This may be due to complex interactions between members of the polymicrobial flora and their host BEC.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Rudney JD,Chen Rdoi
10.1016/j.archoralbio.2005.09.003subject
Has Abstractpub_date
2006-04-01 00:00:00pages
291-8issue
4eissn
0003-9969issn
1879-1506pii
S0003-9969(05)00236-0journal_volume
51pub_type
杂志文章abstract：:Notch receptors are involved in cell fate decisions through the process of lateral inhibition or inductive signalling. Jagged2 belongs to the family of transmembrane proteins that serve as the ligands for Notch receptors. We have analysed the expression of the Jagged2 gene in developing mouse teeth. Jagged2 expression...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2004.10.006
更新日期：2005-02-01 00:00:00
abstract：:Using monoclonal antibodies reacting with T-cell subpopulations, Langerhans cells and macrophages, the number and distribution of cells of the immune system in normal oral and cervical mucosa was determined and statistically compared with that in oral papillomas and oral leukoplakias. Increased numbers of labelled cel...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90042-1
更新日期：1985-01-01 00:00:00
abstract：:Designing a temporomandibular joint (TMJ) total prosthesis requires the assessment of joint displacements for open/close movements. Current knowledge presents disc-condyle motions as involving both translation and rotation but there may be substantial variations from human to human. The aim of this study is to discrim...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2007.11.010
更新日期：2008-05-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to compare tooth components (enamel and dentin) in Familial Dysautonomia (FD) and Down syndrome (DS) in order to assess the extent to which each was affected. DESIGN:The design was cross-sectional. The sample consisted of 20 FD patients and 45 DS patients. The control group comp...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2004.02.008
更新日期：2004-08-01 00:00:00
abstract：:With scanning electron microscopy, globules were seen in inter-cellular spaces between ameloblasts with ruffled borders and papillary cells at early maturation stage. Numerous globules were located on longitudinal folds between parallel ridges on the sides of ameloblasts, but only a few in the papillary layer. In prev...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(84)90121-3
更新日期：1984-01-01 00:00:00
abstract：OBJECTIVE:To compare the mineralisation density (MD), morphology and histology of alveolar bone and cementum amongst VDR +/+, VDR -/-, and VDR -/- groups supplemented with a diet TD 96348, containing 20% lactose, 2.0% calcium and 1.25% phosphorous. METHODS:Four groups of mice (6 mice/group) were identified by genotypi...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.12.004
更新日期：2011-07-01 00:00:00
abstract：OBJECTIVE:The aim of this study is to explore patterns of non-syndromic permanent tooth agenesis in a large orthodontic patient group. DESIGN:A record review was performed in various orthodontic clinics to identify white patients with non-syndromic permanent tooth agenesis, excluding 3rd molars. Four hundred and fourt...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.02.020
更新日期：2017-07-01 00:00:00
abstract：:The apparent diffusion rate, D, of lactate was significantly retarded in dental plaque fluid and a simulated plaque fluid consisting of a chemically-defined solution of salts, amino acids and albumin in phosphate buffer at pH 6.5. Metabolic utilization of lactate in live plaque residue reduced D for lactate into such ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90106-2
更新日期：1985-01-01 00:00:00
abstract：OBJECTIVES:Reconstruct childhood diet using teeth collected in Edmonton in a collaborative effort between the Departments of Dentistry and Anthropology at the University of Alberta. METHODS:Deciduous teeth needing extraction were collected from 33 children for stable isotope ratio analysis of diet. Tooth dentin was mi...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.07.014
更新日期：2014-11-01 00:00:00
abstract：:Ureaplasmas were detected in the saliva of 3.5 per cent (12/342) of subjects. A total of 60 isolated strains did not revert to bacterial forms on repeated subculture in antibacterial agent-free media, failed to grow in serum-free media, were inhibited by digitonin and hydrolysed urea but not glucose or arginine. Twent...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90188-7
更新日期：1983-01-01 00:00:00
abstract：:The mRNA for insulin-like growth factor I (IGF-I) in the submandibular gland of mature and developing rats was examined by Northern blotting and in situ hybridization with an oligonucleotide probe. In the mature adult rat, IGF-I mRNA was expressed at a higher level in the submandibular gland than in the liver, and was...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90006-8
更新日期：1993-08-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to investigate the factors that could participate on salivary glands hypofunction during inflammation and the participation of endocannabinoids in hyposalivation induced by the presence of inflammogens in the submandibular gland (SMG) or in the brain. DESIGN:Salivary secretion was a...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2013.04.003
更新日期：2013-09-01 00:00:00
abstract：OBJECTIVE:To evaluate the expression of matrix metalloproteinase inducer (EMMPRIN) and its correlation with the expression of matrix metalloproteinases (MMPs)-1, -2 and -9 during the development of periapical lesion in mice. METHODS:Periapical lesions were induced in the lower first molars of mice and after 7, 14, 21 ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.05.021
更新日期：2014-09-01 00:00:00
abstract：:Cementum of teeth previously analysed for fluoride was re-examined in order to determine whether or not periodontal disease had affected the thickness of the tissue. In the periodontally diseased teeth the cementum was thinner than normal in the middle region of the root. The apical cementum, however, was significantl...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(92)90132-r
更新日期：1992-08-01 00:00:00
abstract：:After an initial demineralisation, an intensive fluoridation is capable of inhibiting the erosive mineral loss in dentine completely, which might be related to the presence of the exposed organic dentine matrix. Aim of the present study was, therefore, to evaluate whether fluoride is also effective when the organic ma...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2004.05.010
更新日期：2004-11-01 00:00:00
abstract：OBJECTIVE:To test the association of child's growth/development status and socioeconomic variables with dental emergence patterns. DESIGN:A sample of 359 children belonging to a birth cohort of over 5000 children born in 1993 in Pelotas (Brazil) was studied in 1998-1999. Previously trained observers collected birthwei...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2006.12.001
更新日期：2007-06-01 00:00:00
abstract：OBJECTIVES:The holy grail of biomarker research in periodontology is to develop a high impact diagnostics which have a significant impact on clinical decision-making, patient outcomes and healthcare providers. In the field of periodontal diagnostics, oral fluid-based biomarkers have been studied mainly in the gingival ...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/j.archoralbio.2017.12.022
更新日期：2018-03-01 00:00:00
abstract：:Mandibular first-molar tooth germs were dissected from 16-day-embryo and new-born CD1 mice. By incubation in collagenase they were separated into enamel organ, papilla and follicle. Dental pulp was obtained from mandibular first molars of 3-, 7- and 10-day-old mice. Various combinations of epithelial and mesenchymal t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(87)90022-7
更新日期：1987-01-01 00:00:00
abstract：:The retrognathic Brachyrrhine (Br) heterozygote mouse mutant has a very localized morphological deficiency in the sphenoethmoidal region of the anterior cranial base. The purpose of this study was to test the hypothesis that a primary growth defect occurs in that region of Br mice. Primary cell cultures were derived f...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(01)00105-4
更新日期：2002-02-01 00:00:00
abstract：:The aim of this study was to compare total IgA in the whole saliva of children with Down syndrome with levels in sibling and parent groups. IgA measurements were presented as the concentration in saliva (μg/ml) and also adjusted for salivary flow rate (SFR; μg/min). Twenty children with Down syndrome, ten siblings and...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.03.005
更新日期：2016-07-01 00:00:00
abstract：OBJECTIVE:It has been suggested that oxidative stress plays a role in the pathogenesis of chronic obstructive pulmonary disease (COPD), though this role has yet to be fully elucidated. The purpose of this study was to further evaluate this role as concomitantly expressed in the saliva and broncho-alveolar lavage (BAL/'...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2006.08.002
更新日期：2007-01-01 00:00:00
abstract：:The expression and distribution of inducible nitric oxide synthase (iNOS) was examined in 12 samples of human temporomandibular joint (TMJ) with internal derangement (ID) and four control specimens. In the diseased joints, strong or definite iNOS reactivity was expressed in synovial lining and endothelial cells; weake...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(00)00086-8
更新日期：2001-01-01 00:00:00
abstract：:The rate of synthesis of secretory proteins increases significantly in rat parotid glands after stimulated discharge of stored proteins. How any difference in the amount of secretory protein discharge affects the rate of subsequent protein synthesis, and whether this post-secretory synthesis is regulated at the level ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(89)90147-7
更新日期：1989-01-01 00:00:00
abstract：:Samples of gingival crevicular fluid (GCF) were collected in 30 volunteers with inflamed gingiva, using either capillary tubes (cGCF) or Durapore strips (sGCF). They were examined, together with samples of serum from the same patients, by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and/or by two-dimensi...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(96)00076-3
更新日期：1996-08-01 00:00:00
abstract：:Dentinal fluid volume and protein concentrations were measured from cavities prepared in the dentine of dog molars. Fluid was collected under spontaneous conditions, during the application of negative pressures, and during a recovery period. The data allowed the calculation of dentine permeability as hydraulic conduct...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(91)90072-3
更新日期：1991-01-01 00:00:00
abstract：OBJECTIVE:The existence of extra-pineal melatonin has been observed in various tissues. No prior studies of melatonin in human oral mucosal tissue under the condition of chronic inflammation have been reported. The aim of this study was to investigate the presence of melatonin in oral mucosal tissue of patients with or...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.02.007
更新日期：2017-06-01 00:00:00
abstract：OBJECTIVE:We studied the relationship between chewing rhythmicity, craniomandibular morphology, and age in humans. DESIGN:Sixty subjects (10M:10F/group×three age groups, viz., 4-8, 10-14, and 17-21 years) participated. Subjects chewed gum for 2min while jaw movements in the frontal plane were videorecorded. Mean and v...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.04.011
更新日期：2014-07-01 00:00:00
abstract：OBJECTIVE:As shown in the quantitative suspension test adding lactoperoxidase to a thiocyanate (SCN(-)) hydrogen peroxide (H(2)O(2)) combination over the physiological saliva level has significant positive antimicrobial effects to a level of totally killing Streptococcus mutans, Streptococcus sanguinis, and Candida alb...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.04.016
更新日期：2011-12-01 00:00:00
abstract：OBJECTIVES:To explore the role of rostral ventromedial medulla (RVM) orexin 1 receptors (OX1R) on orofacial nociception -induced anxiety and locomotion in rats. DESIGN:Forty two adult male Wistar rats (220-270 gr) were randomly divided into 7 groups (n = 6) as follows: untreated control, capsaicin, capsaicin vehicle-t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104778
更新日期：2020-08-01 00:00:00
abstract：OBJECTIVE:To investigate the association between passive smoking, as determined by salivary cotinine level, and caries, salivary pH, buffering capacity, flow rate and microbiological counts in young children. DESIGN:Ninety passive smokers (PS) with a mean age of 5.02 years and 90 healthy age-matched controls were incl...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2008.05.007
更新日期：2008-10-01 00:00:00
