Abstract：
:Myeloid leukemias in AKXD23 mice contain proviral insertions at Evi1, resulting in transcriptional activation. Although Evi1 is clearly involved in leukemia, gene transfer studies in mice with Evi1 fail to cause leukemia, arguing that cooperating events are necessary. We reanalyzed AKXD-23 tumors for cooperating proviral insertion and found that each tumor had a proviral insertion in Sox4, which encodes an HMG-box transcription factor. RNA analysis revealed these insertions cause increased Sox4 expression. Overexpression of Sox4 in 32Dcl3 cells markedly inhibited cytokine-induced granulocyte maturation, as documented by morphologic and mRNA analysis. Sox4-expressing cells had higher levels of transcripts associated with proliferation, including Evi1. Conversely, in leukemic cells that express Sox4 and bear provirally activated Evi1, suppression of Sox4 with short hairpin RNAs resulted in down-regulation of both Sox4 and Evi1. By cotransfection studies, Sox4 is able to transactivate the AKV long terminal repeat, which likely explains how Sox4 transcriptionally up-regulates provirally activated Evi1; however, Sox4 does not appear to regulate the native Evi1 promoter. We propose that Sox4 proviral activation is selected for in the setting of prior proviral activation of Evi1, because it transactivates the relatively weak LTR of AKV leading to higher Evi1 expression and consequent block to differentiation.
journal_name
Bloodjournal_title
Bloodauthors
Boyd KE,Xiao YY,Fan K,Poholek A,Copeland NG,Jenkins NA,Perkins ASdoi
10.1182/blood-2003-05-1626subject
Has Abstractpub_date
2006-01-15 00:00:00pages
733-41issue
2eissn
0006-4971issn
1528-0020pii
2003-05-1626journal_volume
107pub_type
杂志文章相关文献
BLOOD文献大全abstract：:The regenerative capacity of hematopoietic stem cells (HSCs) is limited by the accumulation of DNA damage. Conditional mutagenesis of the histone 3 lysine 4 (H3K4) methyltransferase, Setd1a, revealed that it is required for the expression of DNA damage recognition and repair pathways in HSCs. Specific deletion of Setd...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2017-09-806844
更新日期：2018-03-22 00:00:00
abstract：:An in vitro system has been developed that mimics the passage of erythrocytes from the bone marrow to the circulation. Bone marrow egress and its proper regulation are vital physiologic processes. However, because of the inaccessibility of the marrow, it is difficult to evaluate the various factors important in contro...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1986-07-01 00:00:00
abstract：:Tissue type plasminogen activator (t-PA) is an effective thrombolytic agent in experimental animals. The duration of the thrombolytic effect of infused t-PA is unknown. We compared the duration of the thrombolytic effect of t-PA with streptokinase by measuring the lysis of 125I-fibrin-labeled thrombi in rabbit jugular...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1985-08-01 00:00:00
abstract：:The influence of the shape of the red blood cell during stomatocyte-echinocyte transformation on its deformability was studied by microsieving through pores with diameters of 2.6, 4.5, and 6.9 micron. A stomatocytic transformation was produced by chlorpromazine (0.02, 0.1, and 0.5 mmol/L) and an echinocytic transforma...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1986-04-01 00:00:00
abstract：:A hybridoma-derived monoclonal antibody, produced by immunization with the Burkitt's tumor-derived B-lymphoblastoid cell line, B35M, was previously shown to detect a 68,000 dalton surface membrane protein, BL2, on the surface of peripheral blood B cells, which is absent from thymocytes, T cells, and granulocytes. In t...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
abstract：:The membrane-anchored CX3C chemokine fractalkine (FKN) is expressed on activated endothelium and is associated with the development of atherosclerosis. The potential of FKN in mediating platelet adhesion beyond platelet activation remains unexplored to date. A flow-based adhesion assay was used to study the adhesion o...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2011-02-335471
更新日期：2011-05-05 00:00:00
abstract：:"Fetal" erythrocytes are present in older children and certain adults with hematologic disorders. To determine if regenerating bone marrow produces such cells, we examined the blood of seven allogeneic bone marrow transplant recipients. Six patients were engrafted with donor cells, while on e patients recovered autolo...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1976-12-01 00:00:00
abstract：:Folylpoly-gamma-gluatamate synthetase (FPGS) catalyzes the polyglutamylation and thus intracellular retention of folates and antifolates (eg, methotrexate; MTX) through the addition of multiple glutamate equivalents to their gamma-carboxyl residue. Since polyglutamylation of antifolates is crucial for their pharmacolo...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2008-08-173799
更新日期：2009-04-30 00:00:00
abstract：:Although thalidomide (Thal) was initially used to treat multiple myeloma (MM) because of its known antiangiogenic effects, the mechanism of its anti-MM activity is unclear. These studies demonstrate clinical activity of Thal against MM that is refractory to conventional therapy and delineate mechanisms of anti-tumor a...
journal_title：Blood
pub_type： 临床试验,杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：:Interleukin-1 (IL-1) has been shown to ameliorate the hematopoietic toxicities of antitumor chemotherapeutic agents in both mice and humans. However, IL-1 toxicity in humans is considerable and is similar to the systemic inflammatory toxicities induced by IL-3, IL-6, and other cytokines with pleiotropic biologic activ...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：:Infections during granulocytopenia are major complications of autologous bone marrow transplantation (ABMT). Since recombinant human granulocyte-macrophage colony-stimulating factor (rhuGM-CSF) has proved to accelerate bone marrow recovery after cytostatic chemotherapy, we studied its effects on hematopoietic regenera...
journal_title：Blood
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：
更新日期：1992-11-01 00:00:00
abstract：:BCR-ABL antisense oligodeoxynucleotides (ODN) have provided evidence of antileukemia effect when tested in vitro against Philadelphia-positive (Ph-pos) cells and in vivo when injected into leukemic mice. On the basis of the results obtained in vitro at diagnosis, eight patients with chronic myelogenous leukemia (CML) ...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：:Combination cytokine therapy continues to be evaluated in an effort to stimulate multilineage hematopoietic reconstitution after bone marrow myelosuppression. This study evaluated the efficacy of combination therapy with the synthetic interleukin-3 receptor agonist, Synthokine-SC55494, and recombinant methionyl human ...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1996-05-15 00:00:00
abstract：:We report the outcome of allogeneic bone marrow transplantation (BMT) as treatment for severe combined immunodeficiency disease (SCID) in 31 patients grafted from 1968 until 1992. The patients received a graft from an HLA-identical related (n = 10), an HLA-haplo-identical related (n = 19), or a closely HLA-matched unr...
journal_title：Blood
pub_type： 杂志文章,评审
doi：
更新日期：1994-12-01 00:00:00
abstract：:Mammalian beta-globin loci contain multiple genes that are activated at different developmental stages. Studies have suggested that the transcription of one gene in a locus can influence the expression of the other locus genes. The prevalent model to explain this transcriptional interference is that all potentially ac...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2006-06-029868
更新日期：2007-03-01 00:00:00
abstract：:Membrane markers expressed by a transplantable granulocytic leukemia induced in inbred strain 13 guinea pigs by N-nitroso-N-butylurea have been investigated by serologic and immunochemical methods. Although the leukemic myeloblasts have no detectable surface immunoglobulin or Fc receptors, they have been found to synt...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1980-02-01 00:00:00
abstract：:The primate erythrocyte (E) complement receptor, CR1, is a transmembrane glycoprotein located in clusters on the surface of E. In vivo studies have demonstrated that during processing and clearance of complement-opsonized immune complexes, large amounts of immunoglobulin G (IgG) can be bound to primate E via CR1 with ...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：:Tartrate-resistant acid phosphatase (TRAP) is an iron-containing protein encoded by the same gene that codes for uteroferrin, a placental iron transport protein. In human peripheral mononuclear cells, TRAP expression is inhibited by both hemin (ferric protoporphyrin IX) and protoporphyrin IX. Nuclear run-on assays con...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1996-09-15 00:00:00
abstract：:In T cell malignancy, rearrangements of chromosome 14 have been observed with a break in the band that contains the alpha chain gene for the T cell receptor (TCR). Because the beta chain TCR gene is in chromosome band 7q34, we searched for and report finding specific rearrangements of 7q34 exclusively in T cell malign...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
abstract：:Neutrophilic differentiation is dependent on CCAAT enhancer-binding protein α (C/EBPα), a transcription factor expressed in multiple organs including the bone marrow. Using functional genomic technologies in combination with clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 ...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2016-01-695759
更新日期：2016-06-16 00:00:00
abstract：:Wnt signaling is involved in numerous aspects of vertebrate development and homeostasis, including the formation and function of blood cells. Here, we show that canonical and noncanonical Wnt signaling pathways are present and functional in megakaryocytes (MKs), with several Wnt effectors displaying MK-restricted expr...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2012-03-416875
更新日期：2013-01-03 00:00:00
abstract：:What are the rules that govern a naive T cell's prospects for survival or division after export from the thymus into the periphery? To help address these questions, we combine data from existing studies with robust mathematical models to estimate the absolute contributions of thymopoiesis, peripheral division, and los...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2008-10-184184
更新日期：2009-05-28 00:00:00
abstract：:Pretreatment of endothelial cells with cytokines enhances the adherence of leukocytes, a process that is mediated by surface proteins expressed on both cell types. A three-dimensional model system for the simultaneous determination of leukocyte adherence and migration was used to study the contribution of CD11/CD18, e...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1991-11-15 00:00:00
abstract：:We have previously determined that in African sickle cell anemia (SS) patients three different beta-like globin gene cluster haplotypes are associated with different percent G gamma (one of the two types of non-alpha chains comprising hemoglobin F [HbF]), mean percent HbF, and percent dense cells. We report now that i...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1991-03-15 00:00:00
abstract：:The NADPH:O2 oxidoreductase catalyzing the respiratory burst in activated phagocytes from healthy individuals is not operative in phagocytes from patients with chronic granulomatous disease (CGD). In a microscopic slide test using the dye nitroblue tetrazolium (NBT), carriers of X-linked CGD can be recognized by a mos...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
abstract：:Intracellular oxidative stress in cells transformed by the BCR-ABL oncogene is associated with increased DNA double-strand breaks. Imprecise repair of these breaks can result in the accumulation of mutations, leading to therapy-related drug resistance and disease progression. Using several BCR-ABL model systems, we fo...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2008-07-172148
更新日期：2009-08-27 00:00:00
abstract：:Global gene expression profiling of highly purified 5q-deleted CD34+CD38(-)Thy1+ cells in 5q- myelodysplastic syndromes (MDSs) supported that they might originate from and outcompete normal CD34+CD38(-)Thy1+ hematopoietic stem cells. Few but distinct differences in gene expression distinguished MDS and normal stem cel...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2007-03-079368
更新日期：2007-10-15 00:00:00
abstract：:Parasitic worms of the genus Schistosoma excrete relatively large amounts of immunogenic glycoproteins (circulating cathodic antigen [CCA]) that contain polysaccharide side chains with the trisaccharide Lewis-x (L(ex)) as a repeating unit. These carbohydrates evoke high titers of specific IgM antibodies that cross-rea...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:Human natural killer (NK) cell development is a step-by-step process characterized by phenotypically identified stages. CD161 is a marker informative of the NK cell lineage commitment, whereas CD56, CD117, and CD94/NKG2A contribute to define discrete differentiation stages. In cells undergoing in vitro differentiation...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2011-09-379693
更新日期：2012-04-26 00:00:00
abstract：:Microparticles (MPs) are submicron extracellular vesicles exposing phosphatidylserine (PS), detected at high concentration in the circulation of sickle cell anemia (SS) patients. Several groups studied the biological effects of MPs generated ex vivo. Here, we analyzed for the first time the impact of circulating MPs o...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood.2020004853
更新日期：2020-07-09 00:00:00
