Abstract:
:We found that caffeine significantly inhibited epidermal growth factor (EGF)- and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell transformation in the JB6 mouse epidermal cell line. The tumor promoter-induced cell transformation was also blocked by treatment with an adenosine A1 receptor antagonist, 8-phenyltheophylline (8-PTH). Caffeine slightly attenuated activation of EGF-induced activator protein 1 (AP-1) activation, which play important roles in cell transformation, but only at the highest concentration examined (1 mM). Interestingly, pretreatment with caffeine suppressed EGF-induced phosphorylation and activation of Akt and ribosomal p 70 S6 protein kinase (p 70 S 6 K), a target of Akt, without inhibiting phosphatidylinositol 3-kinase (PI 3 K) activation. The inhibition of Akt activation of caffeine was not a result of its adenosine receptor antagonism. Because Akt plays a key role in signal transduction pathways leading to cell proliferation and apoptosis, our results provide novel insight into possible mechanisms of the chemotherapeutic effect of caffeine.
journal_name
Mol Carcinogjournal_title
Molecular carcinogenesisauthors
Nomura M,Ichimatsu D,Moritani S,Koyama I,Dong Z,Yokogawa K,Miyamoto Kdoi
10.1002/mc.20120subject
Has Abstractpub_date
2005-09-01 00:00:00pages
67-76issue
1eissn
0899-1987issn
1098-2744journal_volume
44pub_type
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pub_type: 杂志文章
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更新日期:2015-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2016-05-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2009-04-01 00:00:00
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journal_title:Molecular carcinogenesis
pub_type: 杂志文章
doi:10.1002/mc.21989
更新日期:2014-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mc.10098
更新日期:2003-02-01 00:00:00
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更新日期:2003-06-01 00:00:00
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更新日期:2014-01-01 00:00:00
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更新日期:2014-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mc.20233
更新日期:2007-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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更新日期:2007-08-01 00:00:00
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