当前位置： SCI文献检索 > CANCER RESEARCH期刊下所有文献 > Multiepitope HER2 targeting enhances photoimmunotherapy of HER2-overexpressing cancer cells with pyropheophorbide-a immunoconjugates.

Multiepitope HER2 targeting enhances photoimmunotherapy of HER2-overexpressing cancer cells with pyropheophorbide-a immunoconjugates.

Abstract：

:Multi-targeting strategies improve the efficacy of antibody and immunotoxin therapies but have not yet been thoroughly explored for HER2-based cancer treatments. We investigated multi-epitope HER2 targeting to boost photosensitizer immunoconjugate uptake as a way of enhancing photoimmunotherapy. Photoimmunotherapy may allow targeted photodynamic destruction of malignancies and may also potentiate anticancer antibodies. However, one obstacle preventing its clinical use is the delivery of enough photosensitizer immunoconjugates to target cells. Anti-HER2 photosensitizer immunoconjugates were constructed from two monoclonal antibodies (mAb), HER50 and HER66, using a novel method originally developed to label photosensitizer immunoconjugates with the photosensitizer, benzoporphyrin derivative verteporfin. Photosensitizer immunoconjugates were labeled instead with a promising alternative photosensitizer, pyropheophorbide-a (PPa), which required only minor changes to the conjugation procedure. Uptake and phototoxicity experiments using human cancer cells were conducted with the photosensitizer immunoconjugates and, for comparison, with free PPa. SK-BR-3 and SK-OV-3 cells served as HER2-overexpressing target cells. MDA-MB-468 cells served as HER2-nonexpressing control cells. Photosensitizer immunoconjugates with PPa/mAb molar ratios up to approximately 10 specifically targeted and photodynamically killed HER2-overexpressing cells. On a per mole basis, photosensitizer immunoconjugates were less phototoxic than free PPa, but photosensitizer immunoconjugates were selective for target cells whereas free PPa was not. Multiepitope targeted photoimmunotherapy with a HER50 and HER66 photosensitizer immunoconjugate mixture was significantly more effective than single-epitope targeted photoimmunotherapy with a single anti-HER2 photosensitizer immunoconjugate, provided photosensitizer immunoconjugate binding was saturated. This study shows that multiepitope targeting enhances HER2-targeted photoimmunotherapy and maintains a high degree of specificity. Consequently, it seems that multitargeted photoimmunotherapy should also be useful against cancers that overexpress other receptors.

journal_name

Cancer Res

journal_title

Cancer research

authors

Savellano MD,Pogue BW,Hoopes PJ,Vitetta ES,Paulsen KD

doi

10.1158/0008-5472.CAN-05-0426

subject

Has Abstract

pub_date

2005-07-15 00:00:00

pages

6371-9

issue

14

eissn

0008-5472

issn

1538-7445

pii

65/14/6371

journal_volume

65

pub_type

杂志文章

相关文献

CANCER RESEARCH文献大全
  • Proteasome-mediated degradation and functions of hematopoietic progenitor kinase 1 in pancreatic cancer.

    abstract：:Hematopoietic progenitor kinase 1 (HPK1) regulates stress responses, proliferation, and apoptosis in hematopoietic cells. In this study, we examined the expression, regulation, and functions of HPK1 in pancreatic ductal adenocarcinomas (PDA). We found that loss of HPK1 protein expression correlated significantly with ...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：10.1158/0008-5472.CAN-08-1751

    authors： Wang H,Song X,Logsdon C,Zhou G,Evans DB,Abbruzzese JL,Hamilton SR,Tan TH,Wang H

    更新日期：2009-02-01 00:00:00

  • Stromal cell-derived factor 1alpha stimulates human glioblastoma cell growth through the activation of both extracellular signal-regulated kinases 1/2 and Akt.

    abstract：:In this paper, we describe the role of chemokine receptor CXCR4 activation by its natural ligand, the chemokine stromal cell-derived factor (SDF-1) (CXCL12), in glioblastoma cell growth in vitro. We show that both CXC chemokine receptor 4 (CXCR4) and SDF-1 mRNA are expressed in several human glioblastoma multiforme tu...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Barbero S,Bonavia R,Bajetto A,Porcile C,Pirani P,Ravetti JL,Zona GL,Spaziante R,Florio T,Schettini G

    更新日期：2003-04-15 00:00:00

  • Prognosis is correlated with p53 mutation type for soft tissue sarcoma patients.

    abstract：:We investigated the prognostic value of p53 mutation type for 145 soft tissue sarcoma patients. In a PCR-SSCP-sequencing analysis, 15 mutations were identified: 10 non-frameshift (non-fs) and 5 frameshift (fs) mutations. Patients possessing non-fs mutations had a significantly poorer prognosis than patients without p5...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Taubert H,Meye A,Würl P

    更新日期：1996-09-15 00:00:00

  • Purification and properties of hamster liver ligandins, glutathione S-transferases.

    abstract：:Glutathione S-transferases have been purified to homogeneity from Chinese hamster liver. Three enzyme forms were separated and designated Forms I, II, and III in order of their elution from carboxymethylcellulose columns. The forms exhibit close physical similarities to glutathione S-transferases B (ligandin) of rat l...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Smith GJ,Ohl VS,Litwack G

    更新日期：1980-06-01 00:00:00

  • Activation of nuclear factor kappaB In vivo selectively protects the murine small intestine against ionizing radiation-induced damage.

    abstract：:Exposure of mice to total body irradiation induces nuclear factor kappaB (NFkappaB) activation in a tissue-specific manner. In addition to the spleen, lymph nodes, and bone marrow, the tissues that exhibit NFkappaB activation now include the newly identified site of the intestinal epithelial cells. NFkappaB activated ...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：10.1158/0008-5472.CAN-04-0591

    authors： Wang Y,Meng A,Lang H,Brown SA,Konopa JL,Kindy MS,Schmiedt RA,Thompson JS,Zhou D

    更新日期：2004-09-01 00:00:00

  • Towards Greater Inclusion in Cancer Genomics Studies.

    abstract：:The study by Jaratlerdsiri and colleagues represents the first whole-genome sequencing study of prostate cancer from men in sub-Saharan Africa. Preliminary findings include a higher tumor mutation burden in these tumors compared with data from other available prostate cancer cohorts. Although larger studies are needed...

    journal_title：Cancer research

    pub_type： 评论,杂志文章

    doi：10.1158/0008-5472.CAN-18-3382

    authors： Huang FW

    更新日期：2018-12-15 00:00:00

  • Overexpression of the insulin-like growth factor-1 receptor and autocrine stimulation in human cervical cancer cells.

    abstract：:We characterized mechanisms of growth control involving insulin-like growth factor-1 (IGF-1), IGF-2, and IGF-1 receptor (IGF-1R) by investigating their expression in human cervical cancer cell lines, primary cervical tumor cell cultures, and normal ectocervical epithelial cells maintained in short-term culture. By rev...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Steller MA,Delgado CH,Bartels CJ,Woodworth CD,Zou Z

    更新日期：1996-04-15 00:00:00

  • Characterization of the CA 125 antigen associated with human epithelial ovarian carcinomas.

    abstract：:The murine monoclonal antibody OC125 reacts with an antigenic determinant (CA 125) found on a high-molecular-weight glycoprotein complex present in the serum of greater than 80% of women with epithelial ovarian cancer. The antigen expressing CA 125 (CA 125 antigen) isolated from the sera of ovarian carcinoma patients ...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Davis HM,Zurawski VR Jr,Bast RC Jr,Klug TL

    更新日期：1986-12-01 00:00:00

  • Pharmacokinetic and phase I evaluation of esorubicin (4'-deoxydoxorubicin) by continuous infusion over forty-eight hours in patients with leukemia.

    abstract：:Sixteen patients with previously treated acute nonlymphocytic leukemia or chronic myelogenous leukemia in blast crisis were given one to three courses of esorubicin by continuous infusion over 48 h. Dosage levels extended from 35 to 85 mg/m2. Four patients showed partial responses of short duration. Nonhematological t...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Kreis W,Rottach C,Budman DR,Chan K,Schulman P,Allen SL,Weiselberg L,Vinciguerra V

    更新日期：1988-10-01 00:00:00

  • The c-Fes protein-tyrosine kinase suppresses cytokine-independent outgrowth of myeloid leukemia cells induced by Bcr-Abl.

    abstract：:The c-Fes protein-tyrosine kinase exhibits strong expression in myeloid hematopoietic cells. Previous studies have shown that Fes induces differentiation in the chronic myelogenous leukemia-derived cell line K-562, suggesting that the Fes signal for differentiation is dominant to the Bcr-Abl signal for transformation ...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Lionberger JM,Smithgall TE

    更新日期：2000-02-15 00:00:00

  • Growth inhibition of human breast cancer cells in vitro with an antibody against the type I somatomedin receptor.

    abstract：:Insulin and insulin-like growth factors (IGFs) stimulate the growth of human breast cancer cells in vitro. The type I somatomedin receptor (SR), expressed in these cells, may mediate the mitogenic effects of these peptides. We have examined the effect of type I SR blockade on human breast cancer growth with a monoclon...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Arteaga CL,Osborne CK

    更新日期：1989-11-15 00:00:00

  • Continuous infusion of high-dose 1-beta-D-arabinofuranosylcytosine: a phase I and pharmacological study.

    abstract：:High doses of 1-beta-D-arabinofuranosylcytosine, administered as a continuous i.v. infusion, were evaluated in a Phase I trial in 14 patients with advanced solid tumors. 1-beta-D-Arabinofuranosylcytosine was given at 250 mg/sq m/h for infusions of 12 to 36 h. The mean steady state 1-beta-D-arabinofuranosylcytosine pla...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Spriggs DR,Robbins G,Takvorian T,Kufe DW

    更新日期：1985-08-01 00:00:00

  • Activation of Rap1 promotes prostate cancer metastasis.

    abstract：:Elucidating the mechanisms of prostate cancer (CaP) survival and metastasis are critical to the discovery of novel therapeutic targets. The monomeric G protein Rap1 has been implicated in cancer tumorigenesis. Rap1 signals to pathways involved in cell adhesion, migration, and survival, suggesting Rap1 may promote seve...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：10.1158/0008-5472.CAN-08-4269

    authors： Bailey CL,Kelly P,Casey PJ

    更新日期：2009-06-15 00:00:00

  • Immortalization of Syrian hamster embryo cells is in itself a multistep event.

    abstract：:The hypothesis that induction of immortalization of rodent cells follows one-hit kinetics was tested by the determination of frequencies of immortalization of Syrian hamster embryo cells after treatment with benzo(a)pyrene, X-rays, or ethylnitrosourea. Contrary to expectation, immortalization did not occur in a single...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Bols BL,Naaktgeboren JM,Simons JW

    更新日期：1991-02-15 00:00:00

  • Growth inhibition of human melanoma cells in nude mice by antisense strategies to the type 1 insulin-like growth factor receptor.

    abstract：:The growth of human melanoma cells FO-1 in nude mice is strongly inhibited or even abrogated when the cells are stably transfected with a plasmid expressing an antisense RNA to the insulin-like growth factor 1 receptor (IGF-1R) RNA, which causes a marked reduction in the number of IGF-1 receptors. When a tumor arises ...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Resnicoff M,Coppola D,Sell C,Rubin R,Ferrone S,Baserga R

    更新日期：1994-09-15 00:00:00

  • Plasminogen activators and tumor development in the human colon: activity levels in normal mucosa, adenomatous polyps, and adenocarcinomas.

    abstract：:Malignant changes are often accompanied by alterations in activity and composition of the plasminogen activators (PA). To study the relationship between PA expression and the development of colorectal cancer, we determined urokinase-type plasminogen activator (u-PA) and tissue-type plasminogen activator (t-PA) activit...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： de Bruin PA,Griffioen G,Verspaget HW,Verheijen JH,Lamers CB

    更新日期：1987-09-01 00:00:00

  • Molecular cytogenetic mapping of the human melanoma antigen (MAGE) gene family to chromosome region Xq27-qter: implications for MAGE immunotherapy.

    abstract：:We have defined the chromosomal location of the human MAGE (melanoma antigen) gene family by polymerase chain reaction amplification of several segments of the MAGE genes from somatic cell hybrids containing well-defined groups of human chromosomes and hybrids containing human derivative chromosomes. The data show tha...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Oaks MK,Hanson JP Jr,O'Malley DP

    更新日期：1994-04-01 00:00:00

  • Dietary administration of the proapoptotic vitamin E analogue alpha-tocopheryloxyacetic acid inhibits metastatic murine breast cancer.

    abstract：:The ability of the vitamin E (RRR-alpha-tocopherol) derivatives alpha-tocopheryl succinate (alpha-TOS) and alpha-tocopheryloxyacetic acid (alpha-TEA) to suppress tumor growth in preclinical animal models has recently led to increased interest in their potential use for treating human cancer. To make the use of these v...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：10.1158/0008-5472.CAN-06-2403

    authors： Hahn T,Szabo L,Gold M,Ramanathapuram L,Hurley LH,Akporiaye ET

    更新日期：2006-10-01 00:00:00

  • LYN is a mediator of epithelial-mesenchymal transition and a target of dasatinib in breast cancer.

    abstract：:Epithelial-mesenchymal transition (EMT), a switch of polarized epithelial cells to a migratory, fibroblastoid phenotype, is considered a key process driving tumor cell invasiveness and metastasis. Using breast cancer cell lines as a model system, we sought to discover gene expression signatures of EMT with clinical an...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：10.1158/0008-5472.CAN-09-3141

    authors： Choi YL,Bocanegra M,Kwon MJ,Shin YK,Nam SJ,Yang JH,Kao J,Godwin AK,Pollack JR

    更新日期：2010-03-15 00:00:00

  • Development of Chemotherapy with Cell-Cycle Inhibitors for Adult and Pediatric Cancer Therapy.

    abstract：:Preclinical and clinical development of agents that inhibit cell-cycle progression have brought an understanding of the feasibility of targeting various cell-cycle regulators in patients with cancer. Small molecule inhibitors targeting key proteins that participate in cell-cycle progression including the cyclin-depend...

    journal_title：Cancer research

    pub_type： 杂志文章,评审

    doi：10.1158/0008-5472.CAN-17-2782

    authors： Mills CC,Kolb EA,Sampson VB

    更新日期：2018-01-15 00:00:00

  • Identify metastasis-associated genes in hepatocellular carcinoma through clonality delineation for multinodular tumor.

    abstract：:Disease recurrence and metastasis are frequently observed in many successfullytreated localized cancers, including hepatocellular carcinoma in which intrahepatic and extrahepatic recurrence (metastasis) are frequently observed after curative resection. The present study aimed at identifying metastasis-associated genes...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Cheung ST,Chen X,Guan XY,Wong SY,Tai LS,Ng IO,So S,Fan ST

    更新日期：2002-08-15 00:00:00

  • Isolation of variant cells from SV-40 transformed human diploid fibroblasts.

    abstract：:Variant cell lines that are sensitive to density-dependent inhibition of growth have been isolated from three of four SV40-transformed human fibroblast cell lines. Variants were isolated by plating the transformed cells at low density, by treating them with 5-fluorodeoxyuridine, or by growing them on glutaraldehyde-fi...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Rosenberg N,Schaeffer WI,Diamond L

    更新日期：1975-08-01 00:00:00

  • Overexpression and mutation of p53 in endometrial carcinoma.

    abstract：:Immunohistochemical staining for the p53 protein was performed in 107 snap frozen primary endometrial adenocarcinomas and 15 benign uterine tissues using monoclonal antibody PAb1801. No staining was seen in benign samples, whereas intense nuclear staining of cancer cells consistent with overexpression of the p53 prote...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Kohler MF,Berchuck A,Davidoff AM,Humphrey PA,Dodge RK,Iglehart JD,Soper JT,Clarke-Pearson DL,Bast RC Jr,Marks JR

    更新日期：1992-03-15 00:00:00

  • Direct evidence for a role of cyclooxygenase 2-derived prostaglandin E2 in human head and neck xenograft tumors.

    abstract：:Both nonsteroidal anti-inflammatory drugs (NSAIDs) and cyclooxygenase (COX) 2-selective inhibitors such as celecoxib are being reported as having potent anticancer activity in laboratory models. Several reports have suggested that the mechanism of action of these agents in reducing tumor volume/burden is unrelated to ...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Zweifel BS,Davis TW,Ornberg RL,Masferrer JL

    更新日期：2002-11-15 00:00:00

  • CD47 in the tumor microenvironment limits cooperation between antitumor T-cell immunity and radiotherapy.

    abstract：:Although significant advances in radiotherapy have increased its effectiveness in many cancer settings, general strategies to widen the therapeutic window between normal tissue toxicity and malignant tumor destruction would still offer great value. CD47 blockade has been found to confer radioprotection to normal tissu...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：10.1158/0008-5472.CAN-14-0037-T

    authors： Soto-Pantoja DR,Terabe M,Ghosh A,Ridnour LA,DeGraff WG,Wink DA,Berzofsky JA,Roberts DD

    更新日期：2014-12-01 00:00:00

  • Comparison of pharmacokinetics of 5-fluorouracil and 5-fluorouracil with concurrent thymidine infusions in a Phase I trial.

    abstract：:The serum half-life of 5-fluorouracil (5-FUra) in humans is best described as a biexponential decay function, with t1/2 alpha = 7.8 +/- 2.6 (S.E.) min and t1/2 beta = 36.8 +/- 13.5 min during initial courses of this drug alone. Pharmacokinetics of 5-FUra during courses of daily therapy (for 5 days) revealed prolongati...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Kirkwood JM,Ensminger W,Rosowsky A,Papathanasopoulos N,Frei E 3rd

    更新日期：1980-01-01 00:00:00

  • In vitro chemical mutagenesis and viral transformation of a human endothelial cell strain.

    abstract：:We have established and characterized a diploid cell strain of normal human endothelial cells, RuBa 7E. RuBa 7E cells have an average cloning efficiency of 20% during early passages and undergo approximately 50 doublings in vitro before senescing spontaneously. At confluence, RuBa 7E cells form a homogeneous monolayer...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Reznikoff CA,DeMars R

    更新日期：1981-03-01 00:00:00

  • Identification and intracellular location of MAGE-3 gene product.

    abstract：:The human MAGE-3 gene encodes a melanoma antigenic epitope recognized by specific cytotoxic T lymphocytes, but its gene product has not been identified thus far. We produced a recombinant MAGE-3 gene product by expression cloning of the entire reading frame in the context of a fusion protein characterized by a 10-hist...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Kocher T,Schultz-Thater E,Gudat F,Schaefer C,Casorati G,Juretic A,Willimann T,Harder F,Heberer M,Spagnoli GC

    更新日期：1995-06-01 00:00:00

  • Fes tyrosine kinase expression in the tumor niche correlates with enhanced tumor growth, angiogenesis, circulating tumor cells, metastasis, and infiltrating macrophages.

    abstract：:Fes is a protein tyrosine kinase with cell autonomous oncogenic activities that are well established in cell culture and animal models, but its involvement in human cancer has been unclear. Abundant expression of Fes in vascular endothelial cells and myeloid cell lineages prompted us to explore roles for Fes in the tu...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：10.1158/0008-5472.CAN-10-3757

    authors： Zhang S,Chitu V,Stanley ER,Elliott BE,Greer PA

    更新日期：2011-02-15 00:00:00

  • Nonrandom chromosome alterations that correlate with progression to immortality in rat tracheal epithelial cells transformed with N-methyl-N'-nitro-N-nitrosoguanidine.

    abstract：:Primary rat tracheal epithelial cells can be transformed in vitro by N-methyl-N'-nitro-N-nitrosoguanidine. The earliest recognizable morphological transformant is the enhanced growth variant (EGV), characterized by enhanced proliferative capacity. Transformed EGV colonies can progress to give rise to immortal cell lin...

    journal_title：Cancer research

    pub_type： 杂志文章

    doi：

    authors： Endo S,Nettesheim P,Oshimura M,Walker C

    更新日期：1990-02-01 00:00:00