Hibernation induction trigger reduces hypoxic damage of swine skeletal muscle.

Abstract:

:A link between the cardioprotective benefits of pharmacological preconditioning and natural mammalian hibernation is considered to involve the cellular activation of opioid receptors and subsequent opening of K(ATP) channels. In previous studies, we have demonstrated the protective effects of specific delta-opioid agonists against porcine cardiac ischemia/reperfusion injury. We hypothesize here that preincubation with hibernation induction trigger (HIT) should confer a similar protection in skeletal muscles. Therefore, muscle bundles from swine were pretreated with plasma from hibernating woodchucks (HWP) for 30 min, then exposed to hypoxia for 90 min and reoxygenation for 120 min. Stimulated twitch forces were assessed. The functional effects of pretreatment with nonhibernation (summer) woodchuck plasma, a K(ATP) blocker, or opioid antagonist were also studied. During the reoxygenation period, significantly greater force recoveries were observed only for bundles pretreated with HWP; this response was blocked by naloxone (P < 0.05). We conclude that HIT pretreatment could be used to confer protection against hypoxia/reperfusion injury of skeletal muscles of nonhibernators; it could potentially be utilized to prevent injury during surgical procedures requiring ischemia.

journal_name

Muscle Nerve

journal_title

Muscle & nerve

authors

Hong J,Sigg DC,Coles JA Jr,Oeltgen PR,Harlow HJ,Soule CL,Iaizzo PA

doi

10.1002/mus.20354

subject

Has Abstract

pub_date

2005-08-01 00:00:00

pages

200-7

issue

2

eissn

0148-639X

issn

1097-4598

journal_volume

32

pub_type

杂志文章
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