Abstract：
:We evaluated the use of polymerase chain reaction (PCR) for diagnosis of mucosal leishmaniasis (ML) in an endemic area in Acre, Brazil, where Leishmania braziliensis is present. Leishmania DNA was detected 34 of 35 cases, yielding a positivity rate of 97.1%, which was higher than the positivity rates for all of the other diagnostic methods studied, namely Montenegro skin test (MST), anti-Leishmania serological testing and microscopic examination of lesion biopsy specimens. These findings have led us to propose guidelines for the diagnosis of ML that use PCR as the principal method of parasitological confirmation of cases.
journal_name
Acta Tropjournal_title
Acta tropicaauthors
Oliveira JG,Novais FO,de Oliveira CI,da Cruz Junior AC,Campos LF,da Rocha AV,Boaventura V,Noronha A,Costa JM,Barral Adoi
10.1016/j.actatropica.2004.12.003subject
Has Abstractpub_date
2005-04-01 00:00:00pages
55-9issue
1eissn
0001-706Xissn
1873-6254pii
S0001-706X(05)00036-7journal_volume
94pub_type
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