Regulated exocytosis and kiss-and-run of synaptic-like microvesicles in INS-1 and primary rat beta-cells.

abstract：OBJECTIVE:Polymerase I and transcript release factor (PTRF) is a protein highly expressed in adipose tissue and is an integral structural component of caveolae. Here, we report on a novel role of PTRF in lipid mobilization. RESEARCH DESIGN AND METHODS:PTRF expression was examined in different adipose depots of mice du...

journal_title：Diabetes

authors： Aboulaich N,Chui PC,Asara JM,Flier JS,Maratos-Flier E

更新日期：2011-03-01 00:00:00

abstract：:Rats from four experimental treatment groups, including fed controls, 24- to 30-h fasted, dexamethasone-treated, and intraperitoneal glucose-treated, were used to assess the effects of these treatments on the immunohistochemically detectable islet amyloid polypeptide (IAPP) content in the pancreatic islets. Isolated p...

journal_title：Diabetes

authors： O'Brien TD,Westermark P,Johnson KH

更新日期：1991-12-01 00:00:00

abstract：:Improvement of glycemic status by insulin is associated with profound changes in amino acid metabolism in type 1 diabetes. In contrast, a dissociation of insulin effect on glucose and amino acid metabolism has been reported in type 2 diabetes. Type 2 diabetic patients are reported to have reduced muscle oxidative enzy...

journal_title：Diabetes

authors： Halvatsiotis P,Short KR,Bigelow M,Nair KS

更新日期：2002-08-01 00:00:00

abstract：:In patients with diabetes, altered diurnal blood pressure (BP) regulation (high night-to-day [N/D] ratio, or "nondipping") is associated with increases in albumin excretion and a decline in the glomerular filtration rate (GFR) by an unknown mechanism. Because it is known that renin angiotensin system (RAS) activation ...

journal_title：Diabetes

authors： Miller JA,Curtis JR,Sochett EB

更新日期：2003-07-01 00:00:00

abstract：:In early 1988, a colony of GK rats was started in Paris with progenitors issued from F35 of the original colony reported by Goto and Kakisaki. When studied longitudinally up to 8 mo, GK rats showed as early as 1 mo (weaning) significantly higher basal plasma glucose (9 mM) and insulin levels (doubled), altered glucose...

journal_title：Diabetes

authors： Portha B,Serradas P,Bailbé D,Suzuki K,Goto Y,Giroix MH

更新日期：1991-04-01 00:00:00

abstract：:Pancreatic beta-cells are more sensitive to several toxins (e.g., streptozotocin, alloxan, cytokines) than the other three endocrine cell types in the islets of Langerhans. Cytokine-induced free radicals in beta-cells may be involved in beta-cell-specific destruction in type 1 diabetes. To investigate if this sensitiv...

journal_title：Diabetes

authors： Nielsen K,Karlsen AE,Deckert M,Madsen OD,Serup P,Mandrup-Poulsen T,Nerup J

更新日期：1999-12-01 00:00:00

abstract：:Genome-wide nonparametric linkage analysis of 480 sib-pairs affected with type 2 diabetes revealed linkage to a previously unreported susceptibility locus on chromosome 18p11. This result improved with stringent subphenotyping using age- and sex-adjusted BMI, ultimately reaching a logarithm of odds of 3.82 (allele sha...

journal_title：Diabetes

authors： Parker A,Meyer J,Lewitzky S,Rennich JS,Chan G,Thomas JD,Orho-Melander M,Lehtovirta M,Forsblom C,Hyrkkö A,Carlsson M,Lindgren C,Groop LC

更新日期：2001-03-01 00:00:00

abstract：:The interaction between insulin and its receptor was investigated using both monoclonal and polyclonal anti-insulin antibodies. After covalent cross-linking of 125I-insulin to the insulin receptor on cultured human lymphocytes (IM-9 cells) using disuccinimidyl suberate, we inquired whether the insulin-receptor complex...

journal_title：Diabetes

authors： Taylor SI,Schroer JA,Marcus-Samuels B,McElduff A,Bender TP

更新日期：1984-08-01 00:00:00

abstract：:Proliferation of vascular smooth muscle is thought to be involved in the major diabetic complication atherosclerosis. We have previously reported an increase of growth-promoting activity (GA) in platelets from insulin-dependent diabetics. In this study, GA was measured in the platelet extract (PE) from eight diabetic ...

journal_title：Diabetes

authors： Sugimoto H,Franks DJ,Lecavalier L,Chiasson JL,Hamet P

更新日期：1987-05-01 00:00:00

abstract：:Subjects with overt non-insulin-dependent diabetes mellitus (NIDDM) were studied in comparison to obese nondiabetic control subjects and patients with subclinical diabetes. Pancreatic insulin secretion rates were measured by deconvolution of peripheral C-peptide over a 24-h period while subjects consumed an isocaloric...

journal_title：Diabetes

authors： Polonsky KS,Gumbiner B,Ostrega D,Griver K,Tager H,Henry RR

更新日期：1994-07-01 00:00:00

abstract：:Concentrations of glycosylated hemoglobin (GHb) are elevated in diabetes mellitus and are believed to reflect previous metabolic control. To better define possible determinants of GHb in man, we investigated the relationship between GHb and both fasting plasma glucose (FPG) and basal insulin (IRI) in 42 normal subject...

journal_title：Diabetes

authors： Graf RJ,Halter JB,Porte D Jr

更新日期：1978-08-01 00:00:00

abstract：:Heart rate and RR variation (the standard deviation of the mean RR interval for a 5-min period) were evaluated as measurements of cardiac parasympathetic nervous system activity in fasting supine diabetic (N = 22) and comparable age normal (N = 22) subjects. The rate of breathing did not effect heart rate, but was inv...

journal_title：Diabetes

authors： Pfeifer MA,Cook D,Brodsky J,Tice D,Reenan A,Swedine S,Halter JB,Porte D Jr

更新日期：1982-04-01 00:00:00

abstract：:Type 2 diabetes and skeletal muscle insulin resistance have been linked to accumulation of the intramyocellular lipid-intermediate diacylglycerol (DAG). However, recent animal and human studies have questioned such an association. Given that DAG appears in different stereoisomers and has different reactivity in vitro,...

journal_title：Diabetes

authors： Serup AK,Alsted TJ,Jordy AB,Schjerling P,Holm C,Kiens B

更新日期：2016-10-01 00:00:00

abstract：:Hypoxia-inducible factor (HIF)-1 mediates hypoxia- and chronic kidney disease-induced fibrotic events. Here, we assessed whether HIF-1 blockade attenuates the manifestations of diabetic nephropathy in a type 1 diabetic animal model, OVE26. YC-1 [3-(5'-hydroxymethyl-2'-furyl)-1-benzyl indazole], an HIF-1 inhibitor, red...

journal_title：Diabetes

authors： Nayak BK,Shanmugasundaram K,Friedrichs WE,Cavaglierii RC,Patel M,Barnes J,Block K

更新日期：2016-05-01 00:00:00

abstract：:Cardiovascular diseases are the leading cause of morbidity and mortality in diabetes. Lipoprotein lipase (LPL), a major secretory product of macrophages, has been suggested to play a key role in the development of atherosclerosis. In the present study, we evaluated the effect of high glucose on macrophage LPL mRNA exp...

journal_title：Diabetes

authors： Sartippour MR,Lambert A,Laframboise M,St-Jacques P,Renier G

更新日期：1998-03-01 00:00:00

abstract：:T-1095A and T-1095 are synthetic agents derived from phlorizin, a specific inhibitor of Na+-glucose cotransporters (SGLTs). Unlike phlorizin, T-1095 is absorbed into the circulation via oral administration, is metabolized to the active form, T-1095A, and suppresses the activity of SGLTs in the kidney. Orally administe...

journal_title：Diabetes

authors： Oku A,Ueta K,Arakawa K,Ishihara T,Nawano M,Kuronuma Y,Matsumoto M,Saito A,Tsujihara K,Anai M,Asano T,Kanai Y,Endou H

更新日期：1999-09-01 00:00:00

abstract：:The dysregulation of receptor tyrosine kinases (RTKs) in multiple cell types during chronic inflammation is indicative of their pathogenic role in autoimmune diseases. Among the many RTKs, vascular endothelial growth factor receptor (VEGFR) stands out for its multiple effects on immunity, vascularization, and cell mig...

journal_title：Diabetes

authors： Villalta SA,Lang J,Kubeck S,Kabre B,Szot GL,Calderon B,Wasserfall C,Atkinson MA,Brekken RA,Pullen N,Arch RH,Bluestone JA

更新日期：2013-08-01 00:00:00

abstract：OBJECTIVE:Progranulin is an important molecule in inflammatory response. Chronic inflammation is frequently associated with central obesity and associated disturbances; however, the role of circulating progranulin in human obesity, type 2 diabetes, and dyslipidemia is unknown. RESEARCH DESIGN AND METHODS:For the measu...

journal_title：Diabetes

authors： Youn BS,Bang SI,Klöting N,Park JW,Lee N,Oh JE,Pi KB,Lee TH,Ruschke K,Fasshauer M,Stumvoll M,Blüher M

更新日期：2009-03-01 00:00:00

abstract：:The efficacy of gangliosides in enhancing axonal regeneration and maturation in the early stages of diabetic neuropathy was assessed by quantitative analysis of immunostained serial sections of the sciatic nerve. Sprague-Dawley rats were made diabetic with a single injection of alloxan (100 mg/kg). One week later they...

journal_title：Diabetes

authors： Triban C,Guidolin D,Fabris M,Marini P,Schiavinato A,Donà M,Bortolami MC,Di Giamberardino L,Fiori MG

更新日期：1989-08-01 00:00:00

abstract：:Munc13-1 is a diacylglycerol (DAG) receptor that is essential for synaptic vesicle priming. We recently showed that Munc13-1 is expressed in rodent and human islet beta-cells and that its levels are reduced in islets of type 2 diabetic humans and rat models, suggesting that Munc13-1 deficiency contributes to the abnor...

journal_title：Diabetes

authors： Kwan EP,Xie L,Sheu L,Nolan CJ,Prentki M,Betz A,Brose N,Gaisano HY

更新日期：2006-05-01 00:00:00

abstract：:Embryo-culture techniques were used to study the effects of exposure to glucose-deficient culture media on rat embryos during organogenesis. Embryos and their extraembryonic membranes (explants) were cultured either in control media or in media with low or very low glucose concentrations. At the start of the culture t...

journal_title：Diabetes

authors： Ellington SK

更新日期：1987-12-01 00:00:00

abstract：:There is growing interest in more widespread application of isolated islet transplantation for the treatment of type 1 diabetes; however, the sequelae of long-term islet residence within the liver are unknown. We report herein a consequence of intraportal islet transplantation, specifically the development of periport...

journal_title：Diabetes

authors： Markmann JF,Rosen M,Siegelman ES,Soulen MC,Deng S,Barker CF,Naji A

更新日期：2003-07-01 00:00:00

abstract：:The type I and type II isozymes of hexokinase coexist in insulin-sensitive tissues, such as cardiac and skeletal muscle and adipose tissue. Based on an early report that the purified type I isozyme was stable at 45 degrees C whereas the purified type II isozyme was not, investigators in a number of studies have used h...

journal_title：Diabetes

authors： Wilson JE

更新日期：1998-10-01 00:00:00

abstract：:Representative longitudinal muscle strips (6 x 10 mm) from distal small intestine were obtained from rats after 1, 2, and 3 mo of streptozocin-induced diabetes. The strips were stretched to their optimum lengths and subjected to electrical field stimulation (1-ms pulse duration, 30-270 mA, 10 Hz) in the presence of Kr...

journal_title：Diabetes

authors： Nowak TV,Harrington B,Kalbfleisch J

更新日期：1990-08-01 00:00:00

abstract：:Dynamic aspects of whole body alanine and glycine metabolism have been explored in insulin-dependent (type I) diabetic subjects. Using a primed, continuous intravenous (i.v.) infusion of [2H3]alanine and [15N]glycine given simultaneously with [1-13C]leucine, whole body alanine and glycine fluxes and their rates of de ...

journal_title：Diabetes

authors： Robert JJ,Beaufrere B,Koziet J,Desjeux JF,Bier DM,Young VR,Lestradet H

更新日期：1985-01-01 00:00:00

abstract：:This study examined the role of p38 mitogen-activated protein (MAP) kinase in transducing high glucose into deficits in nerve conduction velocity (NCV) that are characteristic of diabetic neuropathy. p38 activation and NCV were measured in streptozocin-induced diabetic rats treated with a p38 inhibitor, an aldose redu...

journal_title：Diabetes

authors： Price SA,Agthong S,Middlemas AB,Tomlinson DR

更新日期：2004-07-01 00:00:00

abstract：:Exercise bypasses insulin resistance to increase glucose uptake in skeletal muscle and therefore represents an important alternative to stimulate glucose uptake in insulin-resistant muscle. Both Rac1 and AMPK have been shown to partly regulate contraction-stimulated muscle glucose uptake, but whether those two signali...

journal_title：Diabetes

authors： Sylow L,Møller LLV,Kleinert M,D'Hulst G,De Groote E,Schjerling P,Steinberg GR,Jensen TE,Richter EA

更新日期：2017-06-01 00:00:00

abstract：:The plasma and tissue concentration of ascorbic acid (AA) is reduced in diabetes. This study was designed to investigate the mechanism and significance of this phenomenon. The low plasma AA concentration of diabetic rats can be normalized by dietary AA supplement (20-40 mg/day), a dosage approximately equal to the max...

journal_title：Diabetes

authors： McLennan S,Yue DK,Fisher E,Capogreco C,Heffernan S,Ross GR,Turtle JR

更新日期：1988-03-01 00:00:00

abstract：:Filtered proteins including insulin are absorbed in the proximal tubule by means of pinocytosis. The first step in this process is binding of the protein to brush border membrane. As it is not known whether absorption exhibits specificity, we set out to determine whether specific binding sites for insulin are present ...

journal_title：Diabetes

authors： Rabkin R,Petersen J,Mamelok R

更新日期：1982-07-01 00:00:00

abstract：:Recently, peptides with opioid-like activity have been demonstrated in peptic digests of dietary protein. The present study was designed to determine the effect of digested and undigested gluten on postprandial insulin and glucagon levels in conscious dogs. The intragastric instillation of digested gluten (25 g) elici...

journal_title：Diabetes

authors： Schusdziarra V,Henrichs I,Holland A,Klier M,Pfeiffer EF

更新日期：1981-04-01 00:00:00