Abstract:
:Mutation rates at microsatellites tend to increase with the number of repeats of the motif, leading to higher levels of polymorphism at long microsatellites. To standardize levels of diversity when microsatellites differ in size, we investigate the relationship between tract length and variation and provide a formula to adjust allelic richness to a fixed mean number of repeats in the specific case of chloroplast microsatellites. A comparison between 39 loci from eight species of conifers (where chloroplast DNA is paternally inherited) and 64 loci from 12 species of angiosperms (where chloroplast DNA is generally predominantly maternally inherited) indicates that the greater allelic richness found in conifers remains significant after controlling for number of repeats. The approach stresses the advantage of reporting variation in number of repeats instead of relative fragment sizes.
journal_name
Mol Ecoljournal_title
Molecular ecologyauthors
Petit RJ,Deguilloux MF,Chat J,Grivet D,Garnier-Géré P,Vendramin GGdoi
10.1111/j.1365-294X.2005.02446.xsubject
Has Abstractpub_date
2005-03-01 00:00:00pages
885-90issue
3eissn
0962-1083issn
1365-294Xpii
MEC2446journal_volume
14pub_type
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