Standardizing for microsatellite length in comparisons of genetic diversity.

Abstract:

:Mutation rates at microsatellites tend to increase with the number of repeats of the motif, leading to higher levels of polymorphism at long microsatellites. To standardize levels of diversity when microsatellites differ in size, we investigate the relationship between tract length and variation and provide a formula to adjust allelic richness to a fixed mean number of repeats in the specific case of chloroplast microsatellites. A comparison between 39 loci from eight species of conifers (where chloroplast DNA is paternally inherited) and 64 loci from 12 species of angiosperms (where chloroplast DNA is generally predominantly maternally inherited) indicates that the greater allelic richness found in conifers remains significant after controlling for number of repeats. The approach stresses the advantage of reporting variation in number of repeats instead of relative fragment sizes.

journal_name

Mol Ecol

journal_title

Molecular ecology

authors

Petit RJ,Deguilloux MF,Chat J,Grivet D,Garnier-Géré P,Vendramin GG

doi

10.1111/j.1365-294X.2005.02446.x

subject

Has Abstract

pub_date

2005-03-01 00:00:00

pages

885-90

issue

3

eissn

0962-1083

issn

1365-294X

pii

MEC2446

journal_volume

14

pub_type

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