Mitochondrial DNA variable number tandem repeats (VNTRs): utility and problems in molecular ecology.

Abstract:

:Analysis of mitochondrial (mt)DNA size polymorphism in the form of variable number tandem repeats (mtVNTRs) has become an increasingly popular methodology for addressing questions in molecular ecology. When detected by PCR, mtVNTR analysis can provide a sensitive, rapid, and cost-effective measure of genetic variability that may be exploited in studies of population differentiation and biogeography. Despite the emergence of this approach, there has been little critical evaluation of its success or utility as a practical tool. In this review, we identify problematic methodological, theoretical and interpretive factors that can influence the utility of mtVNTR analysis. The reliability of the procedure is considered in terms of both detection of alleles and scoring of intra-individual allele frequencies. While many of the potential technical problems of the technique do not raise serious practical concerns, this rapid and sensitive methodology is seriously compromised by the difficulty of reliably assessing allele frequencies, of assaying only germline tissue, and in our ignorance of the mechanisms generating mtVNTR diversity. Thus, although there is a considerable potential for mtVNTR pilot studies to assess genetic diversity, the utility of the technique to resolve broader questions in molecular ecology should be treated cautiously until such a time as the system is better understood.

journal_name

Mol Ecol

journal_title

Molecular ecology

authors

Lunt DH,Whipple LE,Hyman BC

doi

10.1046/j.1365-294x.1998.00495.x

subject

Has Abstract

pub_date

1998-11-01 00:00:00

pages

1441-55

issue

11

eissn

0962-1083

issn

1365-294X

journal_volume

7

pub_type

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