当前位置： SCI文献检索 > AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY期刊下所有文献 > Functional analysis of a mutant estrogen receptor isolated from T47Dco breast cancer cells.

Functional analysis of a mutant estrogen receptor isolated from T47Dco breast cancer cells.

Abstract：

OBJECTIVE:Estrogen receptor-positive cancers that initially respond to hormone therapy often progress to a resistant state. The breast cancer cell line T47Dco is a model for such resistance. It is a polymorphic line, composed of multiple cell populations that demonstrate the presence of mutant estrogen receptors by cloning and sequencing techniques. Our objective was to isolate and analyze the structural and functional characteristics of the T47Dco mutant estrogen receptor complementary deoxyribonucleic acid clones. STUDY DESIGN:We constructed two independent T47Dco complementary deoxyribonucleic acid libraries. We isolated and sequenced T47Dco estrogen receptors and have identified a mutant receptor that is truncated near the end of the deoxyribonucleic acid binding domain. This mutant has now been recreated with site-directed mutagenesis and tested for its ability to bind to deoxyribonucleic acid, dimerize with other estrogen receptors, and activate gene transcription by the chloramphenicol acetyltransferase assay and by gel shift assays. RESULTS:The chloramphenicol acetyltransferase assays reveal that in the absence of estradiol low levels of conversion of chloramphenicol to acetylated products occur when the mutant estrogen receptor is used to activate chloramphenicol acetyltransferase gene transcription, supporting that it has some constitutive function. Also, gel mobility shift assays demonstrate low levels of deoxyribonucleic acid binding with the mutant protein. CONCLUSION:This mutant estrogen receptor may contribute to the estrogen receptor-positive, hormone-resistant phenotype of T47Dco cells by constitutively binding to and activating genes that were previously estradiol dependent.

journal_name

Am J Obstet Gynecol

journal_title

American journal of obstetrics and gynecology

authors

Leslie KK,Tasset DM,Horwitz KB

doi

10.1016/s0002-9378(11)90590-0

subject

Has Abstract

pub_date

1992-04-01 00:00:00

pages

1053-61

issue

4

eissn

0002-9378

issn

1097-6868

pii

S0002-9378(11)90590-0

journal_volume

166

pub_type

杂志文章

相关文献

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY文献大全
  • Contemporary management of preterm premature rupture of membranes (PPROM): a survey of maternal-fetal medicine providers.

    abstract：OBJECTIVE:This study was undertaken to characterize variations in the management for women with preterm premature rupture of membranes (PPROM) among maternal-fetal medicine (MFM) specialists in the context of current recommendations for clinical practice and evidenced-based practice. STUDY DESIGN:We performed a Web-ba...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2004.08.005

    authors： Ramsey PS,Nuthalapaty FS,Lu G,Ramin S,Nuthalapaty ES,Ramin KD

    更新日期：2004-10-01 00:00:00

  • Comparison of bony dimensions at the level of the pelvic floor in women with and without pelvic organ prolapse.

    abstract：OBJECTIVE:We sought to compare bony pelvis dimensions at the level of pelvic support in women with and without pelvic organ prolapse (POP). STUDY DESIGN:Pelvic floor dimensions of 42 white women with POP > 1 cm beyond the hymen were compared with 42 age- and parity-matched women with normal support. Bony landmarks rel...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2008.10.040

    authors： Stein TA,Kaur G,Summers A,Larson KA,DeLancey JO

    更新日期：2009-03-01 00:00:00

  • A role for matrix metalloproteinase-9 in spontaneous rupture of the fetal membranes.

    abstract：OBJECTIVES:Preterm premature rupture of fetal membranes is responsible for 30% to 40% of preterm deliveries. Fetal membranes are composed primarily of collagen. Matrix metalloproteinases are enzymes capable of degrading extracellular matrix macromolecules, including collagens. Expression of matrix metalloproteinase-9 (...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(98)70141-3

    authors： Athayde N,Edwin SS,Romero R,Gomez R,Maymon E,Pacora P,Menon R

    更新日期：1998-11-01 00:00:00

  • Oral contraceptive discontinuation: do side effects matter?

    abstract：OBJECTIVE:The purpose of this study was to assess self-reported side effects in women after they received the oral contraceptive (OC) and to compare discontinuation rates, according to presence or absence of side effects. STUDY DESIGN:The study comprised 1716 women aged <25 years who initiated the OC at 3 publicly fun...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：10.1016/j.ajog.2006.12.015

    authors： Westhoff CL,Heartwell S,Edwards S,Zieman M,Stuart G,Cwiak C,Davis A,Robilotto T,Cushman L,Kalmuss D

    更新日期：2007-04-01 00:00:00

  • Preconceptional antibiotic treatment to prevent preterm birth in women with a previous preterm delivery.

    abstract：:This article addresses the question of whether the uterine cavity is normally sterile and reviews the difficulties in conducting microbiologic studies of the endometrium, the limitations of conventional microbiologic techniques (cultivation-dependent), and the potential contribution of molecular microbiology to examin...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ajog.2005.11.050

    authors： Espinoza J,Erez O,Romero R

    更新日期：2006-03-01 00:00:00

  • Suburethral sling using the transobturator approach: a quality-of-life analysis.

    abstract：OBJECTIVE:The objective of the study was to determine the degree of benefit of a transobturator midurethral polypropylene sling in patients with stress urinary incontinence through a quality-of-life instrument and outcome analysis. STUDY DESIGN:Forty-seven patients completed the validated Individual Incontinence Impac...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2005.07.036

    authors： Lukban JC

    更新日期：2005-12-01 00:00:00

  • Localized increase in nitric oxide production and the expression of nitric oxide synthase isoforms in rat uterus with experimental intrauterine infection.

    abstract：OBJECTIVE:We recently reported that nitric oxide was associated with increased mortality among pregnant rats with intrauterine infection. In our current study we investigated the expression of different isoforms of nitric oxide synthases and nitric oxide in the nonpregnant rat uterus with experimental intrauterine infe...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(99)70499-0

    authors： Fang L,Nowicki BJ,Dong YL,Yallampalli C

    更新日期：1999-09-01 00:00:00

  • The role of the transcerebellar view in the detection of fetal central nervous system anomaly.

    abstract：:The current resolution of antenatal ultrasonography permits the sonologist to visualize and critically evaluate the cerebellum and cisterna magna. Abnormalities in the size and shape of these structures will assist the sonologist in the detection of the majority of central nervous system malformations. ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(91)90686-l

    authors： Hill LM,Martin JG,Fries J,Hixson J

    更新日期：1991-05-01 00:00:00

  • Chlamydia trachomatis: female pelvic infection.

    abstract：:Chlamydia trachomatis is a bacterium that causes both symptomatic and asymptomatic infection. This infection has been associated with severe infections of the upper genital tract, such as salpingitis, which may be responsible for a significant number of cases of ectopic pregnancy and infertility. An unsettled question...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,评审

    doi：10.1016/0002-9378(91)90558-9

    authors： Faro S

    更新日期：1991-06-01 00:00:00

  • Effect of regionalization of endometrial cancer care on site of care and patient travel.

    abstract：BACKGROUND:Complex oncologic surgeries, including those for endometrial cancer, increasingly have been concentrated to greater-volume centers, owing to previous research that has demonstrated associations between greater surgical volume and improved outcomes. There is a potential for concentration of care to have unwan...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2019.07.026

    authors： Knisely A,Huang Y,Melamed A,Tergas AI,St Clair CM,Hou JY,Khoury-Collado F,Ananth CV,Neugut AI,Hershman DL,Wright JD

    更新日期：2020-01-01 00:00:00

  • Medical student experiences with accessing and entering patient information in electronic health records during the obstetrics-gynecology clerkship.

    abstract：BACKGROUND:Medical school graduates should be able to enter information from patient encounters and to write orders and prescriptions in the electronic health record. Studies have shown that, although students often can access electronic health records, some students may receive inadequate preparation for these skills....

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2020.02.004

    authors： Hammoud MM,Foster LM,Cuddy MM,Swanson DB,Wallach PM

    更新日期：2020-09-01 00:00:00

  • Real-time data analysis using a machine learning model significantly improves prediction of successful vaginal deliveries.

    abstract：BACKGROUND:The process of childbirth is one of the most crucial events in the future health and development of the offspring. The vulnerability of parturients and fetuses during the delivery process led to the development of intrapartum monitoring methods and to the emergence of alternative methods of delivery. However...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2020.05.025

    authors： Guedalia J,Lipschuetz M,Novoselsky-Persky M,Cohen SM,Rottenstreich A,Levin G,Yagel S,Unger R,Sompolinsky Y

    更新日期：2020-09-01 00:00:00

  • Vaginal fetal fibronectin as a predictor of spontaneous preterm delivery after multifetal pregnancy reduction.

    abstract：OBJECTIVE:The study was undertaken to assess the validity of vaginal fetal fibronectin assay as a screening test for spontaneous preterm delivery in asymptomatic patients who have undergone multifetal pregnancy reduction (MFPR). STUDY DESIGN:A historic cohort of 63 patients who underwent MFPR between 10 and 14 weeks o...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2003.07.027

    authors： Roman AS,Rebarber A,Lipkind H,Mulholland J,Minior V,Roshan D

    更新日期：2004-01-01 00:00:00

  • Epithelial cells from normal human endometrium express a tumor-associated glycoprotein (TAG-72) epitope in vitro.

    abstract：:Monoclonal antibody B72.3 identifies a tumor-associated glycoprotein (TAG-72) epitope derived from a human breast carcinoma metastasis. Recently, expression of this epitope was noted in normal endometrium during the secretory, but not proliferative, menstrual interval. In light of known hormonal control of normal endo...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(90)91179-g

    authors： Osteen KG,Anderson TL,Hargrove JT,Hill GA,Gorstein F

    更新日期：1990-08-01 00:00:00

  • Plasma adrenocorticotropic hormone and cortisol and adrenal blood flow during sustained hypoxemia in fetal sheep.

    abstract：:We examined the effect of sustained hypoxemia with progressive acidemia on pituitary-adrenal endocrine function (adrenocorticotropic hormone, cortisol) and on adrenal blood flow in fetal sheep. Hypoxemia was induced by the maternal sheep breathing a gas mixture containing 9% oxygen, with 3% carbon dioxide added. Induc...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(86)90169-9

    authors： Challis JR,Richardson BS,Rurak D,Wlodek ME,Patrick JE

    更新日期：1986-12-01 00:00:00

  • Hysteroscopic findings after unsuccessful dilatation and curettage for abnormal uterine bleeding.

    abstract：:In recent years numerous articles have reported on the inaccuracies of the dilatation and curettage procedure, citing a false-negative rate of up to 10% to 15%. Indeed, several articles have reported that, in this era of simplified endoscopy, the blind dilatation and curettage should no longer play a significant role ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(88)90367-5

    authors： Brooks PG,Serden SP

    更新日期：1988-06-01 00:00:00

  • Postpartum ultrasonographic findings associated with placenta accreta.

    abstract：:Postpartum ultrasonographic examination of a patient with a retained placenta showed findings consistent with placenta accreta. The diagnosis was confirmed after surgery with histopathologic examination of the uterus. This case illustrates the possible clinical application of ultrasonography in the postpartum diagnosi...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/s0002-9378(11)91557-9

    authors： Shapiro JL,Sherer DM,Hurley JT,Metlay LA,Amstey MS

    更新日期：1992-09-01 00:00:00

  • A randomized trial of low-dose heparin and intermittent pneumatic calf compression for the prevention of deep venous thrombosis after gynecologic oncology surgery.

    abstract：OBJECTIVE:Our aim was to determine the relative efficacy and complications of low-dose heparin and intermittent pneumatic calf compression for the prevention of postoperative venous thrombosis in patients undergoing surgery for gynecologic malignancy. STUDY DESIGN:Randomized trial comparing 107 patients treated with l...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/0002-9378(93)90360-u

    authors： Clarke-Pearson DL,Synan IS,Dodge R,Soper JT,Berchuck A,Coleman RE

    更新日期：1993-04-01 00:00:00

  • Antibiotic treatment of preterm labor with intact membranes: a multicenter, randomized, double-blinded, placebo-controlled trial.

    abstract：OBJECTIVE:Although an association between subclinical intrauterine infection and preterm birth is well established, there is conflicting evidence regarding the benefits of antibiotic administration to women in preterm labor with intact membranes. We attempted to determine the effect of ampicillin-amoxicillin and erythr...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 临床试验,杂志文章,多中心研究,随机对照试验

    doi：10.1016/0002-9378(93)90003-2

    authors： Romero R,Sibai B,Caritis S,Paul R,Depp R,Rosen M,Klebanoff M,Sabo V,Evans J,Thom E

    更新日期：1993-10-01 00:00:00

  • Outpatient laparoscopy for abdominal and pelvic pain in the United States 1994 through 1996.

    abstract：OBJECTIVE:The purpose of this study was to estimate the frequency at which laparoscopic surgery is performed to treat female pelvic pain. STUDY DESIGN:Using the National Survey of Ambulatory Surgery, we performed a retrospective, cross-sectional study of women who had been diagnosed with abdominal or pelvic pain who u...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2005.09.001

    authors： Tu FF,Beaumont JL

    更新日期：2006-03-01 00:00:00

  • Evaluation of the fetal assessment score in pregnancies at risk for intrauterine hypoxia.

    abstract：OBJECTIVE:Our purpose was to define the diagnostic value of a new fetal assessment score that is based on each of the components of the Apgar score. STUDY DESIGN:A fetal assessment score was established to study the main fetal vital functions: (1) cardiovascular (heart rate, color of the skin in the Apgar score), now ...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(93)90618-s

    authors： Arabin B,Snyjders R,Mohnhaupt A,Ragosch V,Nicolaides K

    更新日期：1993-09-01 00:00:00

  • Comparing pregnancy outcomes and loss rates in elective twin pregnancy reduction with ongoing twin gestations in a large contemporary cohort.

    abstract：BACKGROUND:As compared with singleton gestations, twin pregnancies are associated with a significantly higher risk of preterm birth and maternal complications as well as fetal and neonatal morbidity and mortality. Multifetal pregnancy reduction is a technique developed in the 1980s to reduce the fetal number in higher-...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2019.04.001

    authors： Vieira LA,Warren L,Pan S,Ferrara L,Stone JL

    更新日期：2019-09-01 00:00:00

  • Can women without visible pubococcygeal muscle in MR images still increase urethral closure pressures?

    abstract：OBJECTIVE:The purpose of this study was to determine if the ability to increase maximum urethral closure pressure (MUCP) with a pelvic muscle contraction is impaired in women without pubococcygeal muscle (PCM). STUDY DESIGN:This was a cross-sectional study of continent women comparing those with (n=28) and those witho...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2004.03.082

    authors： Miller JM,Umek WH,Delancey JO,Ashton-Miller JA

    更新日期：2004-07-01 00:00:00

  • The squirrel monkey: an animal model of pelvic relaxation.

    abstract：OBJECTIVE:Casual observations of pelvic prolapse have previously been made in adult female squirrel monkeys. We objectively observed and described the pelvic findings of the adult females in our colony to evaluate their potential as an animal model for studies of pelvic relaxation. STUDY DESIGN:Subsets of a group of 2...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/0002-9378(95)90577-4

    authors： Coates KW,Galan HL,Shull BL,Kuehl TJ

    更新日期：1995-02-01 00:00:00

  • Stillbirths in an urban community in Pakistan.

    abstract：OBJECTIVE:The purpose of this study was to determine stillbirth risk factors and gestational age at delivery in a prospective developing country birth cohort. STUDY DESIGN:At 20-26 weeks of gestation, 1369 Pakistani women were prospectively enrolled in the study; the gestational age was determined by ultrasound evalua...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.ajog.2007.07.012

    authors： Jehan I,McClure EM,Salat S,Rizvi S,Pasha O,Harris H,Moss N,Goldenberg RL

    更新日期：2007-09-01 00:00:00

  • Amniotic fluid induces platelet-neutrophil aggregation and neutrophil activation.

    abstract：OBJECTIVE:Amniotic fluid embolism syndrome is a fatal disease in pregnant women. The exact role of platelets and neutrophils in amniotic fluid embolism syndrome is not clear. We examined whether amniotic fluid could affect platelet-neutrophil aggregation and activation and the possible mechanisms. STUDY DESIGN:Blood s...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1016/j.ajog.2013.01.012

    authors： Chen KB,Chang SS,Tseng YL,Chiu TH,Liao CC,Ho M,Huang GS,Li CY

    更新日期：2013-04-01 00:00:00

  • Spontaneous preterm birth: advances toward the discovery of genetic predisposition.

    abstract：:Evidence from family and twin-based studies provide strong support for a significant contribution of maternal and fetal genetics to the timing of parturition and spontaneous preterm birth. However, there has been only modest success in the discovery of genes predisposing to preterm birth, despite increasing sophistica...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ajog.2017.12.009

    authors： Strauss JF 3rd,Romero R,Gomez-Lopez N,Haymond-Thornburg H,Modi BP,Teves ME,Pearson LN,York TP,Schenkein HA

    更新日期：2018-03-01 00:00:00

  • Perimortem cesarean delivery: were our assumptions correct?

    abstract：OBJECTIVE:The recommendation to perform a perimortem cesarean delivery within 4 minutes of maternal cardiac arrest was introduced in 1986. This recommendation was based on the assumptions that cardiopulmonary resuscitation is ineffective in the third trimester because of aortocaval compression, and that fetal and perha...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ajog.2005.02.038

    authors： Katz V,Balderston K,DeFreest M

    更新日期：2005-06-01 00:00:00

  • Misoprostol for intrauterine device insertion in nulliparous women: a randomized controlled trial.

    abstract：OBJECTIVE:To examine the effects of preprocedure misoprostol on intrauterine device (IUD) placement in nulliparous women. STUDY DESIGN:In this randomized controlled double-blind trial at the University of New Mexico reproductive health clinic, nulliparous women requesting an IUD were randomized to 400 mcg of buccal mi...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.ajog.2013.11.018

    authors： Espey E,Singh RH,Leeman L,Ogburn T,Fowler K,Greene H

    更新日期：2014-03-01 00:00:00

  • Prenatal diagnosis by DNA polymorphism analysis of complete mole with coexisting twin.

    abstract：:Partial mole is distinguishable from complete mole with coexisting normal twin after delivery by DNA polymorphysm analysis. Our patient had chorionic villus sampling of a molar-appearing placenta with coexisting fetus at 12.3 weeks. Absent maternal alleles confirmed a diandrogenetic complete mole. Prenatal DNA diagnos...

    journal_title：American journal of obstetrics and gynecology

    pub_type： 杂志文章

    doi：10.1067/mob.2003.151

    authors： Wax JR,Pinette MG,Chard R,Blackstone J,Do,Cartin A

    更新日期：2003-04-01 00:00:00