Genotype distribution of Candida albicans isolates by 25S intron analysis with regard to invasiveness.

Abstract:

:The aim of this study was to genotype Candida albicans strains isolated from patients with invasive and non-invasive deep-seated infections. For this purpose, 301 C. albicans isolates (81 invasive and 220 non-invasive) were genotyped by using specific PCR primers designed to span the transposable group I intron of the 25S rDNA gene. Fifty-three of the 81 invasive isolates were genotype A (65.4%), eight were genotype B (9.9%) and 20 were genotype C (24.7%), while 98 of the 220 non-invasive isolates were genotype A (44.6%), 46 were genotype B (20.9%) and 76 were genotype C (34.5%). Genotype A was more prevalent among invasive isolates and genotypes B and C were more prevalent among non-invasive isolates (P = 0.0046). Genotypes D and E which represent C. dubliniensis were not found. These results indicate that there may be a relationship between C. albicans genotypes and invasiveness; genotype A being more invasive than others. The presence or absence of the transposable group I intron in the 25S rDNA gene may be important in determining the invasiveness of C. albicans.

journal_name

Mycoses

journal_title

Mycoses

authors

Karahan ZC,Güriz H,Ağirbaşli H,Balaban N,Göçmen JS,Aysev D,Akar N

doi

10.1111/j.1439-0507.2004.01022.x

subject

Has Abstract

pub_date

2004-12-01 00:00:00

pages

465-9

issue

11-12

eissn

0933-7407

issn

1439-0507

pii

MYC1022

journal_volume

47

pub_type

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