Flight muscle-specific expression of act88F: GFP in transgenic Culex quinquefasciatus Say (Diptera: Culicidae).

Abstract:

:A strategy to engineer a strain of Culex mosquitoes refractory to filarial transmission is described. A requirement for success of the strategy is identification of a flight muscle-specific promoter that functions in the mosquito. A GFP marker gene under the control of the promoter region of the Drosophila melanogaster act88F gene was inserted into the genome of Culex quinquefasciatus. Transformation was confirmed by Mendelian genetics. Hybridization of a genomic Southern blot to a radiolabeled probe verified that the entire donor plasmid integrated into the mosquito genome. GFP expression in the transgenic mosquitoes was restricted to the flight muscles.

journal_name

Parasitol Int

authors

Allen ML,Christensen BM

doi

10.1016/j.parint.2004.04.002

subject

Has Abstract

pub_date

2004-12-01 00:00:00

pages

307-14

issue

4

eissn

1383-5769

issn

1873-0329

pii

S1383576904000601

journal_volume

53

pub_type

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