Twisting and untwisting a single DNA molecule covered by RecA protein.

abstract：:Molecular dynamics simulations of RNA-protein complex between Escherichia coli loop E/helix IV (LE/HeIV) rRNA and L25 protein reveal a qualitative agreement between the experimental and simulated structures. The major groove of LE is a prominent rRNA cation-binding site. Divalent cations rigidify the LE major groove g...

journal_title：Biophysical journal

authors： Réblová K,Spacková N,Koca J,Leontis NB,Sponer J

更新日期：2004-11-01 00:00:00

abstract：:We have used fluorescence-quenching measurements to characterize the partitioning of a variety of indolyl-labeled phospho- and sphingolipids between gel or liquid-ordered and liquid-disordered lipid domains in several types of lipid bilayers where such domains coexist. In both cholesterol-free and cholesterol-containi...

journal_title：Biophysical journal

authors： Wang TY,Silvius JR

更新日期：2003-01-01 00:00:00

abstract：:We argue that membrane viscosity, η(m), plays a prominent role in the thermal fluctuation dynamics of micron-scale lipid domains. A theoretical expression is presented for the timescales of domain shape relaxation, which reduces to the well-known η(m) = 0 result of Stone and McConnell in the limit of large domain size...

journal_title：Biophysical journal

authors： Camley BA,Esposito C,Baumgart T,Brown FL

更新日期：2010-09-22 00:00:00

abstract：:Segmentation of the Drosophila melanogaster embryo results from the dynamic establishment of spatial mRNA and protein patterns. Here, we exploit recent temporal mRNA and protein expression measurements on the full surface of the blastoderm to calibrate a dynamical model of the gap gene network on the entire embryo cor...

journal_title：Biophysical journal

authors： Bieler J,Pozzorini C,Naef F

更新日期：2011-07-20 00:00:00

abstract：:A simple technique for forming "black" lipid bilayer membranes containing negligible amounts of alkyl solvent is described. The membranes are formed by the method of Mueller et al (Circulation. 1962. 26:1167.) from glyceryl monooleate (GMO) dispersed in squalene. The squalene forms an annulus to satisfy the boundary c...

journal_title：Biophysical journal

authors： White SH

更新日期：1978-09-01 00:00:00

abstract：:Sedimentation velocity is a powerful tool for the analysis of complex solutions of macromolecules. However, sample turbidity imposes an upper limit to the size of molecular complexes currently amenable to such analysis. Furthermore, the breadth of the particle size distribution, combined with possible variations in th...

journal_title：Biophysical journal

authors： Mächtle W

更新日期：1999-02-01 00:00:00

abstract：:Fibrin polymerizes to produce branching fibers forming a three-dimensional network, which has been difficult to visualize by conventional microscopy. Three-dimensional images of whole clots at high resolution were obtained from stereo-pair intermediate-voltage electron micrographs. Computer software was developed to p...

journal_title：Biophysical journal

authors： Baradet TC,Haselgrove JC,Weisel JW

更新日期：1995-04-01 00:00:00

abstract：:We examined model mixed micelles consisting of the nonionic surfactant n-dodecyl-β-D-maltoside, 3-(3-cholamidopropyl)-dimethylammoniopropane sulfonate, and the cholesterol derivative cholesteryl hemisuccinate (CHS) to identify micellar properties that are correlated with the in vitro conformational stability and activ...

journal_title：Biophysical journal

authors： O'Malley MA,Helgeson ME,Wagner NJ,Robinson AS

更新日期：2011-01-19 00:00:00

abstract：:Bioluminescence rhythms from cellular reporters have become the most common method used to quantify oscillations in circadian gene expression. These experimental systems can reveal phase and amplitude change resulting from circadian disturbances, and can be used in conjunction with mathematical models to lend further ...

journal_title：Biophysical journal

authors： St John PC,Taylor SR,Abel JH,Doyle FJ 3rd

更新日期：2014-12-02 00:00:00

abstract：:Distinct lipid environments, including lipid rafts, are increasingly recognized as a crucial factor affecting membrane protein function in plasma membranes. Unfortunately, an understanding of their role in membrane protein activation and oligomerization has remained elusive due to the challenge of characterizing these...

journal_title：Biophysical journal

authors： Siegel AP,Kimble-Hill A,Garg S,Jordan R,Naumann CA

更新日期：2011-10-05 00:00:00

abstract：:This study presents what is, to our knowledge, a novel technique by means of which the ratio of the single gating charges of voltage-gated rat brain IIA (rBIIA) sodium and Shaker potassium ion channels was estimated. In the experiment, multiple tandems of enhanced green fluorescent protein were constructed and inserte...

journal_title：Biophysical journal

authors： Gamal El-Din TM,Grögler D,Lehmann C,Heldstab H,Greeff NG

更新日期：2008-08-01 00:00:00

abstract：:The effect of aggregation of gramicidin A' (GA) on the phase structure of dipalmitoylphosphatidylcholine (DPPC) multilamellar vesicles was studied by cw-ESR using a chain-labeled lipid (16PC) at temperatures between 30 degrees and 45 degreesC that span the main phase transition of DPPC. Boundary lipids were observed o...

journal_title：Biophysical journal

authors： Ge M,Freed JH

更新日期：1999-01-01 00:00:00

abstract：:Plant light-harvesting complex II (LHCII) is the key antenna complex for plant photosynthesis. We present coarse-grained molecular dynamics simulations of monomeric and trimeric LHCII in a realistic thylakoid membrane environment based on the Martini force field. The coarse-grained protein model has been optimized wit...

journal_title：Biophysical journal

authors： Thallmair S,Vainikka PA,Marrink SJ

更新日期：2019-04-23 00:00:00

abstract：:An understanding of the structure-dynamics relationship is essential for understanding how a protein works. Prior research has shown that the activity of a protein correlates with intradomain dynamics occurring at picosecond to millisecond timescales. However, the correlation between interdomain dynamics and the funct...

journal_title：Biophysical journal

authors： Kumar L,Nash A,Harms C,Planas-Iglesias J,Wright D,Klein-Seetharaman J,Sarkar SK

更新日期：2020-07-21 00:00:00

abstract：:The channel-forming properties of two analogs of gramicidin, gramicidin-ethylenediamine (gram-EDA), and gramicidin-N,N-dimethylethylenediamine (gram-DMEDA) were studied in planar lipid bilayers, using protons as the permeant ion. These peptides have positively charged amino groups tethered to their C-terminal ends via...

journal_title：Biophysical journal

authors： Woolley GA,Zunic V,Karanicolas J,Jaikaran AS,Starostin AV

更新日期：1997-11-01 00:00:00

abstract：:Tagging of individual proteins with genetically encoded fluorescent proteins (FPs) has been used extensively to study localization and interactions in live cells. Recent developments in single-molecule localization microscopy have enabled the dynamic visualization of individual tagged proteins inside living cells. How...

journal_title：Biophysical journal

authors： Ghodke H,Caldas VE,Punter CM,van Oijen AM,Robinson A

更新日期：2016-07-12 00:00:00

abstract：:Open channel properties of canine cardiac Purkinje cell Na+ channels were studied with single channel cell-attached recording and with whole cell macroscopic current recording in internally perfused cells. Single channel currents and membrane currents increased with an increase in Na+ concentration, but showed evidenc...

journal_title：Biophysical journal

authors： Sheets MF,Scanley BE,Hanck DA,Makielski JC,Fozzard HA

更新日期：1987-07-01 00:00:00

abstract：:The effects of different cations on the hydrodynamic radius (RH) of a 48-bp synthetic DNA are measured by time-resolved fluorescence polarization anisotropy of intercalated ethidium. Relative statistical errors in RH are only approximately 1%. With increasing cation concentration, Na+ causes a small decrease in RH, Cs...

journal_title：Biophysical journal

authors： Fujimoto BS,Miller JM,Ribeiro NS,Schurr JM

更新日期：1994-07-01 00:00:00

abstract：:We describe the use of asymmetric Ru-ligand complexes as a new class of luminescent probes that can be used to measure rotational motions of proteins. These complexes are known to display luminescent lifetimes ranging from 10 to 4000 ns. In this report, we show that the asymmetric complex Ru(bpy)2(dcbpy) (PF6)2 displa...

journal_title：Biophysical journal

authors： Terpetschnig E,Szmacinski H,Malak H,Lakowicz JR

更新日期：1995-01-01 00:00:00

abstract：:We developed a kinetic model for a single-substrate trimolecular enzymatic system, where a receptor binds and stretches a substrate to expose its cleavage site, allowing an enzyme to bind and cleave it into product. We demonstrated that the general kinetics of the trimolecular enzymatic system is more complex than the...

journal_title：Biophysical journal

authors： Chen W,Zhu C

更新日期：2010-05-19 00:00:00

abstract：:The kinetics of porin incorporation into black lipid membranes (BLM) made of phosphatidylinositol (PI) or oxidized cholesterol (Ox Ch) were studied by means of alternating current; the set-up was able to acquire resistance and capacitance simultaneously by means of a mixed double-frequency approach at 1 Hz and 1 KHz, ...

journal_title：Biophysical journal

authors： Gallucci E,Micelli S,Monticelli G

更新日期：1996-08-01 00:00:00

abstract：:Molecular diffusion process after the photo-induced electron injection to ferric cytochrome c (Fe(III) cyt c) in guanidine hydrochloride (GdnHCl) 3.5 M buffer solution is studied by the time-resolved transient grating technique. Circular dichroism studies have revealed that Fe(III) cyt c is unfolded under this conditi...

journal_title：Biophysical journal

authors： Nada T,Terazima M

更新日期：2003-09-01 00:00:00

abstract：:Cationic amino acid transporters are highly selective for L-enantiomers such as L-arginine (L-Arg). Because of this stereoselectivity, little is known about the interaction of these transporters with D-isomers. To study whether these compounds can provide information on the molecular mechanism of transport, inward cur...

journal_title：Biophysical journal

authors： Zhou J,Peluffo RD

更新日期：2010-11-17 00:00:00

abstract：:A complete description of protein structure and function must include a proper treatment of mechanisms that lead to cooperativity. The helix/coil transition serves as a simple example of a cooperative folding process, commonly described by a nucleation-propagation mechanism. The prevalent view is that coil structure m...

journal_title：Biophysical journal

authors： Vorov OK,Livesay DR,Jacobs DJ

更新日期：2009-12-02 00:00:00

abstract：:In multicellular organisms, single-fluorophore imaging is obstructed by high background. To achieve a signal/noise ratio conducive to single-molecule imaging, we adapted reflected light-sheet microscopy (RLSM) to image highly opaque late-stage Drosophila embryos. Alignment steps were modified by means of commercially ...

journal_title：Biophysical journal

authors： Greiss F,Deligiannaki M,Jung C,Gaul U,Braun D

更新日期：2016-02-23 00:00:00

abstract：:Cochlear outer hair cells undergo reversible changes in shape when externally stimulated. This response, known as OHC motility, is a central component of the cochlear amplifier, the mechanism responsible for the high sensitivity of mammalian hearing. We report that actin depolymerization, as regulated by activation/in...

journal_title：Biophysical journal

authors： Matsumoto N,Kitani R,Maricle A,Mueller M,Kalinec F

更新日期：2010-10-06 00:00:00

abstract：:The nucleus has a smooth, regular appearance in normal cells, and its shape is greatly altered in human pathologies. Yet, how the cell establishes nuclear shape is not well understood. We imaged the dynamics of nuclear shaping in NIH3T3 fibroblasts. Nuclei translated toward the substratum and began flattening during t...

journal_title：Biophysical journal

authors： Li Y,Lovett D,Zhang Q,Neelam S,Kuchibhotla RA,Zhu R,Gundersen GG,Lele TP,Dickinson RB

更新日期：2015-08-18 00:00:00

abstract：:Cell motion is driven by interplay between the actin cytoskeleton and the cell adhesions in the front part of the cell. The actin network segregates into lamellipodium and lamellum, whereas the adhesion complexes are characteristically distributed underneath the actin system. Here, we suggest a computational model for...

journal_title：Biophysical journal

authors： Shemesh T,Bershadsky AD,Kozlov MM

更新日期：2012-04-18 00:00:00

abstract：:Many essential processes in eukaryotic cells depend on regulated molecular exchange between its two major compartments, the cytoplasm and the nucleus. In general, nuclear import of macromolecular complexes is dependent on specific peptide signals and their recognition by receptors that mediate translocation through th...

journal_title：Biophysical journal

authors： Salman H,Abu-Arish A,Oliel S,Loyter A,Klafter J,Granek R,Elbaum M

更新日期：2005-09-01 00:00:00

abstract：:Recent work has introduced a new fluorescent voltage sensor, ASAP1, which can monitor rapid trains of action potentials in cultured neurons. This indicator is based on the Gallus gallus voltage-sensitive phosphatase with the phosphatase domain removed and a circularly permuted GFP placed in the S3-S4 linker. However, ...

journal_title：Biophysical journal

authors： Lee EEL,Bezanilla F

更新日期：2017-11-21 00:00:00