Simultaneous determination of thiamin and riboflavin in the sea urchin, Paracentrotus lividus, by high-performance liquid chromatography.

Abstract:

:Thiamin and riboflavin were determined in the sea urchin, Paracentrotus lividus, by reversed-phase liquid chromatography with fluorescence detection and a mobile phase consisting of 72% 0.005 M NH(4)OAc and 28% MeOH at a flow rate of 1.450 ml/min. The total analysis time never exceeded 20 min. After acid hydrolysis and enzymatic digestion, thiamin was oxidized to thiochrome and the oxidized fraction was purified and concentrated by solid-phase extraction. Limits of detection were 9 pg/ml and 80 pg/ml for thiamin and riboflavin, respectively. Good results were obtained with respect to repeatability (RSD%<2.4) and recovery (>91.3%).

journal_name

Int J Food Sci Nutr

authors

Rodríguez-Bernaldo De Quirós A,López-Hernández J,Simal-Lozano J

doi

10.1080/09637480310001622323

subject

Has Abstract

pub_date

2004-06-01 00:00:00

pages

259-63

issue

4

eissn

0963-7486

issn

1465-3478

pii

BXK4PCCAY2NMUAE7

journal_volume

55

pub_type

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