Abstract:
:Thiamin and riboflavin were determined in the sea urchin, Paracentrotus lividus, by reversed-phase liquid chromatography with fluorescence detection and a mobile phase consisting of 72% 0.005 M NH(4)OAc and 28% MeOH at a flow rate of 1.450 ml/min. The total analysis time never exceeded 20 min. After acid hydrolysis and enzymatic digestion, thiamin was oxidized to thiochrome and the oxidized fraction was purified and concentrated by solid-phase extraction. Limits of detection were 9 pg/ml and 80 pg/ml for thiamin and riboflavin, respectively. Good results were obtained with respect to repeatability (RSD%<2.4) and recovery (>91.3%).
journal_name
Int J Food Sci Nutrjournal_title
International journal of food sciences and nutritionauthors
Rodríguez-Bernaldo De Quirós A,López-Hernández J,Simal-Lozano Jdoi
10.1080/09637480310001622323subject
Has Abstractpub_date
2004-06-01 00:00:00pages
259-63issue
4eissn
0963-7486issn
1465-3478pii
BXK4PCCAY2NMUAE7journal_volume
55pub_type
杂志文章abstract::Mexico ranks 2nd in adult obesity and 4th in milk intake worldwide. Low levels of IGF-1 have been related to obesity and can be reverted by milk intake. The rs6214 polymorphism has been associated with an increase in the expression of IGF-1. Therefore, the aim of the study was to evaluate the association between both,...
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journal_title:International journal of food sciences and nutrition
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journal_title:International journal of food sciences and nutrition
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更新日期:2013-08-01 00:00:00
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