Pectin methylesterase activity and ascorbic acid content from guava fruit, cv. Predilecta, in different phases of development.

Abstract:

:The guava pectin methylesterase (PME) specific activity and vitamin C were assayed in samples from different phases of guava fruit development. The PME enzyme from guava was extracted with borate-acetate buffer, 50 mol/l, pH 8.0, in the presence of NaCl 0.3 mol/l. The results showed PME optimum activity at pH 9 and 95 degrees C, and it is a thermostable enzyme. Guava PME retained 96.8% of activity after 300 min in 90 degrees C. Electrophoresis showed that guava PME contained two isoforms, one with 57 kDa molecular mass. The analyses of the different phases of guava maturation showed that ascorbic acid decreases during the maturation process, but PME activity increases with maturation.

journal_name

Int J Food Sci Nutr

authors

Carvalho AB,De Assis SA,Leite KM,Bach EE,Oliveira OM

doi

10.1080/09637480701752244

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

255-65

issue

3

eissn

0963-7486

issn

1465-3478

pii

791409828

journal_volume

60

pub_type

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