Abstract：
:The aim of this study was to assess, whether polymerase chain reaction (PCR) allows sensitive screening of treatment failure suspicions in areas, where drug resistance against African animal trypanosomosis (AAT) appears to be a problem. PCR was used to detect trypanosome infections prior to, 14 and 28 days after controlled treatment of 738 cattle from 10 villages in Kénédougou, Burkina Faso with isometamidium chloride and diminazene aceturate. Using three sets of primers, PCR was three-four times more sensitive and better at species identification, than standard microscopic examination. The better sensitivity and species specificity of PCR have important advantages for drug resistance studies in the field.
journal_name
Acta Tropjournal_title
Acta tropicaauthors
Gall Y,Woitag T,Bauer B,Sidibe I,McDermott J,Mehlitz D,Clausen PHdoi
10.1016/j.actatropica.2004.04.003subject
Has Abstractpub_date
2004-09-01 00:00:00pages
7-16issue
1eissn
0001-706Xissn
1873-6254pii
S0001706X04000968journal_volume
92pub_type
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