Abstract：
:In this study we standardized a new technical approach in which the target mitochondrial DNA sequence (mtDNA) is amplified using a simple but sensitive PCR method as a tool to detect Plasmodium falciparum and Plasmodium vivax. Specific primers were designed to hybridize with cytochrome c oxidase genes of P. falciparum (cox III) and P. vivax (cox I). Amplification products were obtained for all positive samples, presenting homology only for species-specific mtDNA. Sensitivity and specificity were 100%. The applicability of the method was tested in a cross-sectional study, in which 88 blood samples from individuals naturally exposed to malaria in the Brazilian Amazon region were analyzed. Based on the results, the sensitivity and specificity were 100% and 88.3%, respectively. This simple and sensitive PCR method can be useful in specific situations and in different settings of malaria management, in endemic as well as non-endemic areas (travelers), and in clinical or epidemiological studies, with applications in malaria control programs.
journal_name
Acta Tropjournal_title
Acta tropicaauthors
Cunha MG,Medina TS,Oliveira SG,Marinho AN,Póvoa MM,Ribeiro-dos-Santos AKdoi
10.1016/j.actatropica.2009.02.003subject
Has Abstractpub_date
2009-07-01 00:00:00pages
35-8issue
1eissn
0001-706Xissn
1873-6254pii
S0001-706X(09)00034-5journal_volume
111pub_type
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