Abstract：
:A simple, rapid and inexpensive method for the extraction of DNA from filarial vector, Culex quinquefasciatus, useful in Ssp I PCR assay for xenomonitoring of infection with Wuchereria bancrofti is presented. The DNA extracted by this method was found suitable for PCR detection of W. bancrofti infection in pools of 10-30 mosquitoes. The PCR assay employing the simplified DNA extraction method was evaluated for its sensitivity on field caught Cx. quinquefasciatus, in comparison with the conventional dissection and microscopy technique. When assayed on dissection washings of vector mosquitoes the PCR assay detected 45 pools out of 49 dissection positive pools as positive for infection and hence found to be less sensitive than the conventional technique. The reason for detecting four dissection positive pools as negatives by the PCR assay may be due to the loss of a few numbers of parasites (1-3) present in these pools during the transfer of washings of dissected mosquitoes. The PCR assay detected ten out of 72 dissection negative pools as positives, while it did not detect any of the 62 known negative (laboratory reared, uninfected) mosquito pools as positives. When 38 pools (10 mosquitoes/pool) of intact mosquitoes were assessed for infection by each method, the infection rates obtained by the two methods were almost similar (3.35 and 3.01%, respectively, for conventional method and PCR assay). The results thus show that the DNA extraction method, which is simple, rapid, safe and inexpensive, is efficient to generate DNA from vector mosquitoes useful in PCR assay and hence has potential application in xenomonitoring.
journal_name
Acta Tropjournal_title
Acta tropicaauthors
Vasuki V,Hoti SL,Sadanandane C,Jambulingam Pdoi
10.1016/s0001-706x(02)00267-xsubject
Has Abstractpub_date
2003-04-01 00:00:00pages
109-14issue
1eissn
0001-706Xissn
1873-6254pii
S0001706X0200267Xjournal_volume
86pub_type
杂志文章相关文献
ACTA TROPICA文献大全abstract：:By standardized interviews of people in 23 rural villages, in the Oio region of Guinea Bissau, we collected data on which plant species and plant derived products or methods people use to reduce mosquito biting activity. The following plants were used to reduce numbers of mosquitoes indoors at night: fresh or smoulder...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(98)00083-7
更新日期：1999-01-15 00:00:00
abstract：:We conducted the first meta-analysis of ten Schistosoma haematobium (one published and nine unpublished) and eight Schistosoma mansoni (two published and six unpublished) microsatellite datasets collected from individual schistosome-infected school-children across six sub-Saharan Africa countries. High levels of genet...
journal_title：Acta tropica
pub_type： 杂志文章,meta分析
doi：10.1016/j.actatropica.2012.09.014
更新日期：2013-11-01 00:00:00
abstract：:Three Entamoeba spp. including E. suis, zoonotic E. polecki, and E. histolytica, have been described in pigs to date. However, little is known about the molecular epidemiology of these neglected parasites in pigs globally. In this study we surveyed the occurrence and molecular epidemiology of porcine Entamoeba spp. in...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2018.06.020
更新日期：2018-09-01 00:00:00
abstract：:This paper reviews various imaging techniques in different organs. Ultrasound is the most popular and readily available technique. Special emphasis is given to computerized tomography (CT) and magnetic resonance imaging (MRI) findings and their advantages for a confident diagnosis and treatment. ...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/s0001-706x(97)00052-1
更新日期：1997-09-15 00:00:00
abstract：:Mixed viral infections are ubiquitous in natural populations and may have significant but unpredictable biological and epidemiological consequences. These infections may be acquired simultaneously (coinfection) or result from two single infections at different time intervals (superinfection). This study investigated t...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2013.10.016
更新日期：2014-02-01 00:00:00
abstract：:Phlebotomine sand flies are known vectors of several pathogens. In Spain, Phlebotomus perniciosus and Phlebotomus ariasi are the proven vectors of Leishmania infantum. Since 2010 a human leishmaniasis outbreak has been notified in Madrid region, central Spain. Studies have shown that P. perniciosus is the only vector ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2018.08.011
更新日期：2018-11-01 00:00:00
abstract：:Definitions of the term 'challenge' as applied to the African trypanosomiases are reviewed. Data from one West and one East African site show simple linear relationships between the incidence of trypanosomiasis in both humans and animals, and either the amount of man-tsetse contact, or the Apparent Density of flies. D...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：
更新日期：1985-03-01 00:00:00
abstract：:An adaptation of a modified procedure using anion-exchange centrifugation was evaluated to isolate and freeze in field conditions a high number of bloodstream forms of Trypanosoma brucei gambiense for direct in vitro culture. Pellets of trypanosomes were obtained from 62 patients in Central Africa. The presence of try...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2003.10.017
更新日期：2004-04-01 00:00:00
abstract：:The aim of this work was to study the composition of the Culicidae community in three environments with different degrees of anthropic disturbance in the province of Chaco. The nonparametric richness estimator ACE was calculated to measure the completeness of the sampling in each environment, and the diversity of each...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2015.12.002
更新日期：2016-04-01 00:00:00
abstract：:During a period of 2 years, the ecology of Bulinus globosus was studied in 8 habitats in two streams near Ifakara, SE-Tanzania. The relative Bulinus densities were followed monthly. Two different methods for estimating snail densities (man/time vs. palmleaf traps) gave comparable results. Bulinus densities were consta...
journal_title：Acta tropica
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
abstract：:Macrophages infected with amastigotes of Leishmania mexicana mexicana as compared to normal macrophages show decreased migration both randomly and through a 5 microns pore in response to a known chemotaxin, an increased ability to pinocytose and an increased bactericidal ability. Unless very heavily parasitized their ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：
更新日期：1983-03-01 00:00:00
abstract：:The emergence of drug-resistance poses a major obstacle to the control of malaria. A homolog of the major multidrug-transporter in mammalian cells was identified, Plasmodium falciparum multidrug resistance protein-1, pfmdr1, also known as the P-glycoprotein homolog 1, Pgh-1. Several studies have demonstrated strong, a...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2005.04.008
更新日期：2005-06-01 00:00:00
abstract：:Cellular immune responses to specific Schistosoma japonicum recombinant and native antigens were investigated in a defined study population of 155 individuals in the Philippines, where data collected from a 3-year observation of exposure, infection and reinfection pattern were used to categorically classify putative '...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(02)00177-8
更新日期：2002-11-01 00:00:00
abstract：:Azithromycin, an azalide analog of erythromycin was assayed for its in vitro activity against multidrug-resistant Plasmodium falciparum K1 strain by measuring the 3H-hypoxanthine incorporation. Azithromycin caused inhibitory effects on the parasite growth with IC50 and IC90 values of 8.4+/-1.2 microM and 26.0+/-0.9 mi...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2006.10.008
更新日期：2006-12-01 00:00:00
abstract：:Accumulation, degradation and excretion by the host of helminth metabolic end products, such as branched-chain volatile fatty acids are poorly studied although they play an appreciable role in host-parasite interactions and, being detectable in excreta, provide a reliable diagnostic test for at least some helminthiase...
journal_title：Acta tropica
pub_type： 杂志文章
doi：
更新日期：1981-12-01 00:00:00
abstract：:The most important mechanism for combating infection using larval therapy depends on larval excretions and secretions (ES). The present work was aimed at evaluating Sarconesiopsis magellanica (Diptera: Calliphoridae) ES antibacterial activity in six bacterial strains (three Gram-positive and three Gram-negative) and c...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2014.04.018
更新日期：2014-08-01 00:00:00
abstract：:In mid-August 2004, an outbreak of a previously unknown fatal disease of camels was reported to Kassala State veterinary authorities. Several areas in the state were visited during August-October 2004 to collect epidemiological data and specimens for diagnosis. Clinically the disease was characterized by sudden death ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2010.08.002
更新日期：2010-11-01 00:00:00
abstract：:The internal classification of the cosmopolitan and medically important genus Culex is thoroughly reviewed and updated to reflect the multitude of taxonomic changes and concepts which have been published since the classification was last compiled by Edwards in 1932. Both formal and informal taxa are included. The clas...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2011.06.005
更新日期：2011-10-01 00:00:00
abstract：:The pathological events in human cerebral malaria are mimicked in the experimental cerebral malaria (ECM) in Plasmodium berghei ANKA (PBA)-infected C57BL/6 mice. Although previously implied in ECM, the kinetics of cytokines and chemokines expression-an essential functional feature for defining causality in ECM develop...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2017.04.025
更新日期：2017-08-01 00:00:00
abstract：:As strictly haematophagous insects, tsetse flies feed on a wide variety of wild and domestic animals. Although these are mainly mammals, some tsetse species also feed on reptiles. The present study investigated whether the odours of several potential natural tsetse hosts may be used as novel attractants to improve the...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(97)00083-1
更新日期：1997-11-01 00:00:00
abstract：:A key tool for the control, elimination, and eradication of Plasmodium vivax is the development of an effective vaccine. The thrombospondin-related adhesion protein (TRAP) is one of the major sporozoite antigens that plays an important role in the invasion of mosquito salivary glands and hepatocytes by sporozoites. Th...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2017.05.026
更新日期：2017-09-01 00:00:00
abstract：:In Burkina Faso, where malaria is hyper-endemic and transmission intensity is very high, the majority of malaria-related morbidity and mortality occurs in children less than 5 years of age. A control measure such as the use of insecticide-treated curtains (ITC) significantly reduces transmission of malaria infection. ...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2003.12.004
更新日期：2004-05-01 00:00:00
abstract：:In this study, Aphanius chantrei's behaviour of feeding on mosquito larvae has been determined under experimental conditions. In groups that have different sizes (first group 27 mm; second group 40 mm; third group 47 mm), larvae consumption has increased with length and weight. During daytime, average larvae consumpti...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2007.04.016
更新日期：2007-06-01 00:00:00
abstract：:Several DNA probes were designed for use in real-time polymerase chain reaction (PCR) assays to target sequence variation within the ribosomal intergenic spacer (IGS) of schistosomes. A sub-section of the IGS (∼300bp) was amplified, with cross-specific primers, after which group-specific fluorescent, locked nucleic ac...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2011.10.019
更新日期：2013-11-01 00:00:00
abstract：:Leptospirosis is an emerging disease, especially in countries with a tropical climate such as Malaysia. A dramatic increase in the number of cases has been reported over the last decade; however, information on the epidemiological trends of this disease is lacking. The objective of this study is to provide an epidemio...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2016.01.031
更新日期：2016-05-01 00:00:00
abstract：:African animal trypanosomosis, transmitted cyclically by tsetse flies or mechanically by other biting flies, causes serious health problems in livestock. Although tsetse infestations have been observed in Blue Nile State in Sudan, tsetse was eradicated in West Kordofan in 1962, and no further studies have been carried...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2019.105302
更新日期：2020-03-01 00:00:00
abstract：:The last decade has seen an increase in investment and concerted efforts by the Malawi Ministry of Health and partners to control malaria disease. This report summarizes what is known about the burden of malaria and the strategies being implemented to control it in Malawi. Over the past 5 years, roll out of treatment ...
journal_title：Acta tropica
pub_type： 杂志文章,评审
doi：10.1016/j.actatropica.2011.06.017
更新日期：2012-03-01 00:00:00
abstract：:A survey of 100 rural households in a village in the Chaco region of Bolivia revealed a serious problem of Taenia solium cysticercosis, with a seroprevalence of 99/447 (22%) in humans and 102/273 (37%) in pigs. Risk factors for humans were being in older age groups, absence of sanitary facilities, poor formal educatio...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/s0001-706x(01)00161-9
更新日期：2001-12-21 00:00:00
abstract：:Along with the determination of malaria infection rate among suspected patients attending hospitals in Hodeidah governorate, the present study evaluated the accuracy of Plasmodium falciparum histidine-rich protein-2 (PfHRP-2)/parasite-specific lactate dehydrogenase (pLDH)-based rapid diagnostic test (RDT) for the diag...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2017.12.006
更新日期：2018-02-01 00:00:00
abstract：:Haemoproteus species (Haemosporida, Haemoproteidae) are cosmopolitan bird blood parasites, which often cause relatively benign infections in adapted avian hosts, but severe and even lethal haemoproteosis might develop due to internal organ damage if these pathogens inhabit non-adapted (wrong) hosts. Haemoproteids of s...
journal_title：Acta tropica
pub_type： 杂志文章
doi：10.1016/j.actatropica.2020.105486
更新日期：2020-07-01 00:00:00
