Abstract：
:An adaptation of a modified procedure using anion-exchange centrifugation was evaluated to isolate and freeze in field conditions a high number of bloodstream forms of Trypanosoma brucei gambiense for direct in vitro culture. Pellets of trypanosomes were obtained from 62 patients in Central Africa. The presence of trypanosomes was detected in all 62 eluates. The isolation system was efficient. Preliminary results of in vitro culture of 10 thawed samples of trypanosomes were highly promising because no fungal or bacterial contamination was noticed even after 2 weeks of culture.
journal_name
Acta Tropjournal_title
Acta tropicaauthors
Truc P,Likeufack CL,Mbongo N,Ebo'o Enyenga Vdoi
10.1016/j.actatropica.2003.10.017subject
Has Abstractpub_date
2004-04-01 00:00:00pages
219-21issue
2eissn
0001-706Xissn
1873-6254pii
S0001706X03003279journal_volume
90pub_type
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