Abstract:
:A synthetic T cell immunogen (TCI) has been designed as a candidate DNA-based vaccine against Human immunodeficiency virus (HIV)-1 using cytotoxic T lymphocytes (CD8(+) CTL) and T-helper lymphocytes (CD4(+) Th) epitopes retrieved from the Los Alamos HIV Molecular Immunology Database. The protein 392 amino acids in length contains about eighty CTL-epitopes, many of which are overlapping and are totally restricted by ten different HLA class I molecules. To be able to detect CTL responses induced by a DNA vaccine in experimental animals, additional epitopes, restricted by mouse and Macaque rhesus major histocompatibility complex (MHC) class I molecules, were included in the target immunogen. The gene encoding the TCI protein was assembled, cloned into vector plasmids and expressed in a prokaryotic and a eukaryotic system. The presence of HIV-1 protein fragments in the immunogen structure was ascertained by ELISA and immunoblotting using panels of HIV-1-positive sera and monoclonal antibodies to p24. It has been demonstrated that DNA vaccine can induce both specific T cell responses (CTL and blast transformation) and specific antibodies in mice immunized with pcDNA-TCI.
journal_name
Vaccinejournal_title
Vaccineauthors
Bazhan SI,Belavin PA,Seregin SV,Danilyuk NK,Babkina IN,Karpenko LI,Nekrasova NA,Lebedev LR,Ignatyev GM,Agafonov AP,Poryvaeva VA,Aborneva IV,Ilyichev AAdoi
10.1016/j.vaccine.2003.09.048subject
Has Abstractpub_date
2004-04-16 00:00:00pages
1672-82issue
13-14eissn
0264-410Xissn
1873-2518pii
S0264410X04000908journal_volume
22pub_type
杂志文章相关文献
VACCINE文献大全abstract:BACKGROUND:High incidence and serotype diversity of invasive pneumococcal disease (IPD) in Indigenous children in remote Australia led to rapid introduction of 7-valent conjugate pneumococcal vaccine (7vPCV) at 2, 4 and 6 months in 2001, followed by 23-valent polysaccharide pneumococcal vaccine (23vPPV) in the second y...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.10.089
更新日期:2015-11-27 00:00:00
abstract::We have compared two types of plasmids for DNA immunization against HTLV-I envelope glycoproteins. One type of plasmid contains the coding DNA of the complete envelope gene of HTLV-I under the control of the CMV promoter with (CMVenvLTR) or without (CMVenv) the tax/rex genes. The second type contains the coding DNA of...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(99)00565-4
更新日期:2000-04-28 00:00:00
abstract::Polymeric nanoparticles (NPs) of poly (lactic-co-glycolic) acid (PLGA) possess adjuvant properties. To date, there are few studies exploring their application as antigen carriers for vaccination of fish. This study presents a preclinical assessment of the early innate and adaptive immune responses in Atlantic salmon f...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.08.087
更新日期:2011-10-26 00:00:00
abstract::The efficacy of a vaccine is based primarily on the adherence of the subject to the immunization schedule. This paper compares the compliance rates (CR) for the third dose of hepatitis B virus (HBV) vaccine given according to one of two vaccination schedules among subjects attending two sexually transmitted disease (S...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1016/0264-410x(95)93130-2
更新日期:1995-02-01 00:00:00
abstract::Hepatitis A caused by hepatitis A virus (HAV) transmitted by the fecal-oral route, results in considerable morbidity and economic loss. Mucosal immunization can be more effective than conventional injection at inducing both local and systemic immunity to HAV. Here we show that co-administration of killed HAV with synt...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.04.015
更新日期:2006-06-19 00:00:00
abstract::We have previously described the generation of HIV-1 V3-specific cytotoxic T-lymphocytes (CTL) responses in BALB/c (H-2d) mice following immunization with Ty-virus-like particles carrying the V3 loop of gp120 (V3-VLPs) without adjuvant. In this study the effects of various adjuvants on CTL induction by V3-VLPs was exa...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(96)00010-2
更新日期:1996-07-01 00:00:00
abstract::This work demonstrates the presence of immune regulatory cells in the cervical lymph nodes draining Bacillus Calmette-Guérin (BCG) vaccinated site on the dorsum of the ear in guinea pigs. It is shown that whole cervical lymph node cells did not proliferate in vitro in the presence of soluble mycobacterial antigens (PP...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.10.009
更新日期:2020-11-10 00:00:00
abstract:BACKGROUND:Surveillance for acute flaccid paralysis with laboratory confirmation has been a key strategy in the global polio eradication initiative, and the laboratory platform established for polio testing has been expanded in many countries to include surveillance for cases of febrile rash illness to identify measles...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.01.004
更新日期:2015-02-25 00:00:00
abstract:BACKGROUND:Japan was verified as having achieved measles elimination by the Measles Regional Verification Commission in the Western Pacific Region in March 2015. Verification of measles elimination implies the absence of continuous endemic transmission. After the last epidemic in 2007 with an estimated 18,000 cases, Ja...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.08.070
更新日期:2015-11-09 00:00:00
abstract:BACKGROUND AND AIMS:Trans-placentally acquired antibodies can protect infants from infection in the first months of life. However, high concentrations of antibody at birth may impact the infant's own immune response to primary immunization. We examine the relationship between concentration of specific antibody to Borde...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.11.104
更新日期:2014-02-12 00:00:00
abstract::Clinical trials with pandemic influenza vaccine candidates have focused on aluminium hydroxide as an adjuvant to boost humoral immune responses. In this study we investigated the effect of aluminium hydroxide on the magnitude and type of immune response induced by whole-inactivated virus (WIV) vaccine. Balb/c mice wer...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.02.063
更新日期:2008-05-02 00:00:00
abstract:BACKGROUND:US-bound refugees undergo required health assessments overseas to identify and treat communicable diseases of public health significance-such as pulmonary tuberculosis-before migration. Immunizations are not required, leaving refugees at risk for vaccine-preventable diseases. In response, the US Centers for ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.10.047
更新日期:2021-01-03 00:00:00
abstract::A randomized, double-blind trial comparing a diphtheria-tetanus-acellular pertussis vaccine (DTaP) (pertussis toxoid and filamentous hemagglutinin) with a whole-cell vaccine (DTwP) was conducted. A case-contact study was nested in the trial to estimate absolute efficacy. From 1990 through 1994, 4181 children were rand...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0264-410x(97)00100-x
更新日期:1997-10-01 00:00:00
abstract:BACKGROUND:A recent mumps outbreak in Israel despite an ongoing national program of a 2-dose universal childhood vaccination policy since 1988, raised questions regarding population immunity among young adults. OBJECTIVE:To assess the seroprevalence of mumps antibodies among young Israeli adults born after 1987 in ord...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.01.103
更新日期:2011-03-24 00:00:00
abstract::Vaccines have drastically reduced the mortality and morbidity of many diseases. However, vaccines have historically been developed empirically, and recent development of vaccines against current pandemics such as HIV and malaria has been met with difficulty. The advent of high-throughput technologies, coupled with sys...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2015.03.072
更新日期:2015-09-29 00:00:00
abstract::While heptavalent pneumococcal conjugate vaccine (PCV) has decreased vaccine type invasive pneumococcal disease (IPD) nationwide, rapid serotype replacement and increasing parapneumonic empyema, has been reported in Utah children. The effect of pediatric vaccination on adults in this population is unknown. We identifi...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.10.009
更新日期:2011-11-15 00:00:00
abstract:BACKGROUND:We aim to determine the vaccination coverage of social and healthcare workers in International sites of Samusocial, providing emergency care to homeless people, and to assess factors associated with having received necessary doses at adulthood. METHODS:Data on immunization coverage of social and healthcare ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.05.014
更新日期:2017-09-18 00:00:00
abstract::Two outbreaks of hepatitis A started almost simultaneously in a maternal school and in a day care centre located at opposite sides of Florence, Italy, at the end of 2002. Both of them originated from immigrant children, and in both cases, hepatitis A was initially not recognised due to aspecific symptoms. While vaccin...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2005.01.044
更新日期:2005-03-18 00:00:00
abstract::This cross-sectional study aimed to assess pertussis seroprevalence among healthy healthcare workers (HCW) of the Children's Hospital of Tunis, Tunisia. During the study period, 236 blood samples were obtained to determine HCW exposure to pertussis. Concentrations of immunoglobulin G (IgG) to pertussis toxin (PT) were...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.11.023
更新日期:2019-01-03 00:00:00
abstract::The current total of AIDS cases in Mexico is 37,000 of which 86% have occurred in men. The major route of transmission is sexual. The campaign to prevent AIDS has fallen into four phases, and has now been extended to other sexually transmitted diseases, including hepatitis B. The first phase (1985-1989) was based arou...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(99)00475-2
更新日期:2000-02-18 00:00:00
abstract::One approach to develop a malaria blood-stage vaccine is to target proteins that play critical roles in the erythrocyte invasion of merozoites. The merozoite surface proteins (MSPs) and the erythrocyte-binding antigens (EBAs) are considered promising vaccine candidates, for they are known to play important roles in er...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.01.010
更新日期:2012-03-02 00:00:00
abstract::It has been known that influenza A virus infection induces a cross-protective immunity against infection by viruses with different subtypes of viral envelope proteins, hemagglutinin (HA) and neuraminidase (NA). This heterosubtypic immunity is generally mediated by cytotoxic T lymphocytes (CTL) reactive to specific epi...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(03)00234-2
更新日期:2003-07-04 00:00:00
abstract::Trehalose-6,6'-dimycolate (TDM) and its monosaccharide-type analogues were synthesized, and their lethal and adjuvant activities were examined in mice. All the monosaccharide-type analogues with a glucose or N-acetylglucosamine moiety were devoid of lethal toxicity to mice; in particular, D-GlcNAcM(1-deoxy) and D-GlcN...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(89)90242-9
更新日期:1989-06-01 00:00:00
abstract::We created and produced a novel self-assembling nanoparticle platform for delivery of peptide epitopes that induces CD8(+) and CD4(+)T cells that are protective against Toxoplasma gondii infection. These self-assembling polypeptide nanoparticles (SAPNs) are composed of linear peptide (LP) monomers which contain two co...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.03.092
更新日期:2014-05-30 00:00:00
abstract:INTRODUCTION:Despite the enormous benefits of childhood and maternal immunisation to individual and population health, the uptake of maternal vaccines during pregnancy remains suboptimal. Midwives are a trusted information source for parents and play an important role in the provision of immunisation information. Under...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.10.006
更新日期:2020-01-10 00:00:00
abstract::Eight different vaccination schemes using four commercially available inactivated Bluetongue vaccines against serotypes 4 and 8 in three different combinations (setting 1-3) were tested under field conditions for their ability to generate a measurable immune response in sheep. Animals of setting 1 (groups A-D) were si...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.08.011
更新日期:2019-09-16 00:00:00
abstract::We have previously shown that vaccination of BALB/c mice with a combination of BCG plus killed Leishmania promastigotes, applied by the i.p. route 10 and 3 days before Trypanosoma cruzi inoculation, prolonged their survival and decreased their parasitaemia. In the present study we show that the BCG-Leishmania vaccine ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(99)00426-0
更新日期:2000-03-06 00:00:00
abstract::On July 9, 2014, Aeras and the Max Planck Institute for Infection Biology convened a workshop entitled "Whole Mycobacteria Cell Vaccines for Tuberculosis" at the Max Planck Institute for Infection Biology on the grounds of the Charité Hospital in Berlin, Germany, close to the laboratory where, in 1882, Robert Koch fir...
journal_title:Vaccine
pub_type:
doi:10.1016/j.vaccine.2015.03.056
更新日期:2015-06-12 00:00:00
abstract::The ELISA method was used to titrate the humoral immune response in vaccinated mice. When mice were given two doses of a heat-killed salmonella vaccine 6 days apart, there was a steady but low-level increase of antibody synthesis. In contrast, if a booster vaccination was administered 21 days after the primary inocula...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(93)90256-w
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:Pregnant women are at risk of severe influenza disease and are a priority group for influenza vaccination programs. Nicaragua expanded recommendations to include influenza vaccination to all pregnant women in the municipality of Managua in 2013. METHODS:We carried out a survey among 1,807 pregnant women who...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.12.065
更新日期:2016-02-17 00:00:00