Abstract：
:Interleukin-2 (IL-2) withdrawal is a physiologic process inducing cell death in activated T lymphocytes. Glucocorticoid-induced leucine zipper (GILZ) has recently been identified as a protein modulating T-cell receptor activation by repressing various signaling pathways. We report here that IL-2 deprivation leads to expression of GILZ in T lymphocytes. We then characterized the human gilz promoter and showed that FoxO3 (Forkhead box class O3) binding to the Forkhead responsive elements identified in the promoter is necessary for induction of gilz expression upon IL-2 withdrawal. To assess the functional consequences of this induction, we used 2 strategies, GILZ overexpression and GILZ silencing in murine IL-2-dependent CTLL-2 cells. GILZ overexpression protects CTLL-2 cells from IL-2 withdrawal-induced apoptosis, whereas cell death is accelerated in cells unable to express GILZ. Concomitantly, the expression of Bim is inhibited in GILZ-overexpressing cells and enhanced when GILZ expression is impaired. Furthermore, GILZ inhibits FoxO3 transcriptional activity that leads to inhibition of Bim expression but also to down-regulation of GILZ itself. Therefore, GILZ is a transiently expressed protein induced upon IL-2 withdrawal that protects T cells from the onset of apoptosis.
journal_name
Bloodjournal_title
Bloodauthors
Asselin-Labat ML,David M,Biola-Vidamment A,Lecoeuche D,Zennaro MC,Bertoglio J,Pallardy Mdoi
10.1182/blood-2003-12-4295subject
Has Abstractpub_date
2004-07-01 00:00:00pages
215-23issue
1eissn
0006-4971issn
1528-0020pii
2003-12-4295journal_volume
104pub_type
杂志文章相关文献
BLOOD文献大全abstract：:The interleukin-4 receptor (IL-4R) is expressed as a 140-Kd membrane glycoprotein that binds IL-4 with high affinity. Recently, cDNA clones for the murine IL-4R have been isolated. One clone encodes an integral membrane protein, while another encodes a protein in which translation is terminated before the transmembran...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:Chronic myeloid leukemia (CML) patients with the BCR-ABL T315I mutation do not benefit from therapy with currently approved tyrosine kinase inhibitors. Omacetaxine mepesuccinate is a protein synthesis inhibitor that has demonstrated activity in cells harboring the T315I mutation. This phase 2 trial assessed the effica...
journal_title：Blood
pub_type： 杂志文章,多中心研究
doi：10.1182/blood-2012-03-415307
更新日期：2012-09-27 00:00:00
abstract：:Advances in fluorescence-activated cell sorter technology have brought about multicolor analysis of cell phenotypes. To clarify the phenotypes of human hematopoietic stem cells (HSCs), we initially prepared novel antibodies against CD34 and labeled one of them (4A1) with allophycocyanin (APC). With this, we analyzed t...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1992-07-15 00:00:00
abstract：:Mammalian beta-globin loci are composed of multiple orthologous genes whose expression is erythroid specific and developmentally regulated. The expression of these genes both from the endogenous locus and from transgenes is strongly influenced by a linked 15-kilobase region of clustered DNaseI hypersensitive sites (HS...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
abstract：:Specific monoclonal and polyclonal antibody reagents and a double antigen indirect immunofluorescence microscopy technique were used to visualize coagulation factor V in human bone marrow. Marrow aspirates were smeared directly on glass slides, or washed and cytospun onto glass slides, or processed and plated into a p...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
abstract：:Cytogenetic and histopathologic data were correlated with clinical parameters from 423 patients with non-Hodgkin's lymphoma (NHL). Clinical correlations were performed on subgroups of 149 patients with low-grade lymphoma (LG) and 205 patients with diffuse lymphoma with a large cell component (DLLC). Correlations were ...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：:The ataxia telangiectasia mutated (ATM)-interacting protein ATMIN mediates noncanonical ATM signaling in response to oxidative and replicative stress conditions. Like ATM, ATMIN can function as a tumor suppressor in the hematopoietic system: deletion of Atmin under the control of CD19-Cre results in B-cell lymphomas i...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2015-09-672980
更新日期：2016-10-20 00:00:00
abstract：:Among the most striking morphological features of acute nonlymphoblastic leukemias (ANLL) is the occurrence of eosinophilic cytoplasmic inclusions known as Auer rods on Auer bodies. We examined immature myeloid cells from the peripheral blood of 9 human fetuses of 16-19 wk gestation for the presence of such structures...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1983-03-01 00:00:00
abstract：:Interleukin-1 beta (IL-1 beta) converting enzyme (ICE) is a cysteine protease that specifically cleaves precursor IL-1 beta to its biologically active form. Recent studies have also implicated ICE in the induction of apoptosis in vertebrate cells. Because IL-1 plays a major role in acute myelogenous leukemia (AML) bla...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1995-12-15 00:00:00
abstract：:Non-Hodgkin lymphoma (NHL) incidence is greatly increased after kidney transplantation. NHL risk was investigated in a nationwide cohort of 8164 kidney transplant recipients registered on the Australia and New Zealand Dialysis and Transplant Registry. NHL diagnoses were ascertained using linkage with national cancer r...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2009-02-202507
更新日期：2009-07-16 00:00:00
abstract：:Hermansky-Pudlak syndrome (HPS) is an inherited hemorrhagic disease affecting the related subcellular organelles platelet dense granules, lysosomes, and melanosomes. The mouse genes for HPS, pale ear and pearl, orthologous to the human HPS1 and HPS2 (ADTB3A) genes, encode a novel protein of unknown function and the be...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：:Transforming growth factor-beta1 (TGF-beta1) has potent physiologic and pathologic effects on a variety of cell types at subnanomolar concentrations. Platelets contain 40 times as much TGF-beta1 as other cells and secrete it as an inactive (latent) form in complex with latency-associated peptide (LAP), which is disulf...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2008-04-151753
更新日期：2008-11-01 00:00:00
abstract：:Human neutrophils possess a very short half-life because they constitutively undergo apoptosis. Cytokines, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), and other agents can rescue neutrophils from apoptosis but the molecular mechanisms involved in this rescue are undefined. Here, we show by Weste...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1998-10-01 00:00:00
abstract：:Previous studies have shown that children with acute myeloid leukemia (AML) who developed mixed chimerism (MC) were at high risk for relapse after allogeneic stem-cell transplantation (allo-SCT). We investigated the feasibility of intensified preemptive immunotherapy in children receiving allo-SCT for AML. Eighty-four...
journal_title：Blood
pub_type： 临床试验,杂志文章
doi：10.1182/blood-2011-04-348805
更新日期：2011-11-17 00:00:00
abstract：:Antigens implicated in the graft-versus-leukemia (GVL) effect in chronic myeloid leukemia (CML) include WT1, PR1, and BCR-ABL. To detect very low frequencies of these antigen-specific CD8+ T cells, we used quantitative polymerase chain reaction (qPCR) to measure interferon-gamma (IFN-gamma) mRNA production by peptide-...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2003-01-0150
更新日期：2003-10-15 00:00:00
abstract：:The outcome of patients with acute myeloid leukemia who are older than 60 years has remained poor because of unfavorable disease characteristics and patient-related factors. The randomized German-Austrian AML Study Group 06-04 protocol was designed on the basis of in vitro synergistic effects of valproic acid (VPA) an...
journal_title：Blood
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1182/blood-2013-12-546283
更新日期：2014-06-26 00:00:00
abstract：:In this issue of Blood, Usmani et al provide important information on second primary malignancies in patients treated with thalidomide and lenalidomide in the Arkansas total therapy trials. ...
journal_title：Blood
pub_type： 评论,杂志文章
doi：10.1182/blood-2012-07-439158
更新日期：2012-08-23 00:00:00
abstract：:Rabbit kidney tissue either from normal rabbits or from rabbits "programmed" by phlebotomy or varying periods of hypoxia was cultured and shown to release into the culture medium an erythropoietically active substance. The cultures established from rabbits programmed by 6-12 hr of hypoxia yielded the highest levels of...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1978-07-01 00:00:00
abstract：:Foxp3(+) regulatory T cells (Tregs) maintain self-tolerance and adoptive therapy, and using Foxp3(+) Tregs has been proposed as treatment for autoimmune diseases. The clinical use of Tregs will require large numbers of cells and methods for in vitro expansion of Tregs are being developed. Foxp3(+) Tregs can be divided...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2011-09-377895
更新日期：2012-03-22 00:00:00
abstract：:The effectiveness of arabinosylcytosine (ara-C) for the treatment of acute myelogenous leukemia (AML) depends on the formation of its active metabolite, the triphosphate of ara-C (ara-CTP). Using biochemical modulation strategies to increase the accumulation of ara-CTP in leukemia blasts, a clinical protocol was desig...
journal_title：Blood
pub_type： 临床试验,杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:With the introduction of genomic technologies, more hereditary cancer syndromes with hematologic malignancies are being described. Up to 10% of hematologic malignancies in children and adults may be the result of an underlying inherited genetic risk. Managing these patients with hereditary hematologic malignancies, in...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2016-06-716704
更新日期：2016-11-24 00:00:00
abstract：:The subcellular localization of the platelet membrane receptors glycoproteins (GP) Ib and IIb/IIIa [corrected] has been studied within resting platelets by a combination of biochemical and cytochemical techniques. While both GPIb and GPIIb/IIIa are localized within the plasma membrane and surface-connected canalicular...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1991-02-15 00:00:00
abstract：:Purified human lactoferrin was assessed for its influence in vivo in untreated mice and in mice undergoing rebound myelopoiesis after sublethal dosages of Cytoxan. Fully iron-saturated lactoferrin suppressed the numbers of granulocytes and monocytes per femur, the numbers of granulocyte-macrophage progenitor cells (CF...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:Follicular dendritic cells (FDCs) form a reticular FDC network in the lymphoid follicle that is essential for the retention and presentation of native antigens in the form of antigen-antibody immune complexes (ICs) to B cells during secondary immune response. Although the presence of migrating precursors of FDCs has b...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2007-01-068387
更新日期：2007-08-15 00:00:00
abstract：:Chronic graft-versus-host disease (cGVHD) after allogeneic hematopoietic stem cell transplant reflects a complex immune response resulting in chronic damage to multiple tissues. Previous studies indicated that donor B cells and the antibodies they produce play an important role in the development of cGVHD. To understa...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2017-08-801001
更新日期：2017-12-28 00:00:00
abstract：:It was found in a long-term bromodeoxyuridine (BrdU) infusion study that two or more different subpopulations of bone marrow stem cells exist in mice. One of these subpopulations appears to be noncycling and forms approximately 10% of eight-day CFU-S. Another one, a subpopulation of slowly cycling bone marrow cells, i...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1985-12-01 00:00:00
abstract：:Human cyclin A1 is a newly cloned, tissue-specific cyclin that is prominently expressed in normal testis. In this study, we showed that cyclin A1 was highly expressed in a subset of leukemia samples from patients. The highest frequency of cyclin A1 overexpression was observed in acute myelocytic leukemias, especially ...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1999-03-15 00:00:00
abstract：:The human erythrocyte actively phosphorylates and dephosphorylates phosphatidylinositol present in the membrane in an apparent "futile cycle." Recent reports have proposed that this phosphorylation/dephosphorylation cycle is a significant consumer of adenosine-5'-triphosphate (ATP) in the erythrocyte. This study detai...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1985-11-01 00:00:00
abstract：:Attempts to maintain or expand primitive hematopoietic stem cells in vitro without the concomitant loss of their differentiative and proliferative potential in vivo have largely been unsuccessful. To investigate this problem, we compared the ability of three cloned bone marrow (BM) stromal cell lines to support the gr...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
abstract：:Human polymorphonuclear leukocytes adhesion to endothelial cells during the early stage of inflammation leads to cell surface externalization of Annexin A1 (AnxA1), an effector of endogenous anti-inflammation. The antiadhesive properties of AnxA1 become operative to finely tune polymorphonuclear leukocytes transmigrat...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2010-02-270520
更新日期：2010-11-18 00:00:00
