Abstract：
:Antitumor vaccination therapy approaches using naked plasmid DNA or recombinant viruses encoding tumor-associated antigens are currently in development. In the present study, we examined the therapeutic efficacy of vaccination using the mouse alpha-fetoprotein (AFP) gene in mouse hepatocellular carcinoma (HCC) cells. C57L/J or C3H/HeN mice were primed with an injection of naked plasmid DNA expressing mouse AFP followed by a booster of replication-defective adenovirus expressing mouse AFP (plasmid-AFP prime/adenovirus-AFP booster vaccination). The mice were then challenged with high AFP-producing Hepa1-6 cells or low AFP-producing MH134 cells, respectively, and the tumor growth rate was monitored. Plasmid-AFP prime/adenovirus-AFP booster vaccination promoted protective immunity against Hepa1-6 cells, and significantly increased the number of interferon-gamma-producing splenic cells in C57L/J mice. In addition, this vaccination protocol repressed the growth of pre-established Hepa1-6 tumors in C57L/J mice. However, plasmid-AFP prime/adenovirus-AFP booster vaccination did not induce protective immunity against MH134 cells in C3H/HeN mice. These results suggest that vaccination with the AFP gene is a promising strategy to treat HCC, but its outcome may be affected by the level of AFP expression in HCC or by the immunological response of the host.
journal_name
Int J Mol Medjournal_title
International journal of molecular medicineauthors
Saeki A,Nakao K,Nagayama Y,Yanagi K,Matsumoto K,Hayashi T,Ishikawa H,Hamasaki K,Ishii N,Eguchi Ksubject
Has Abstractpub_date
2004-01-01 00:00:00pages
111-6issue
1eissn
1107-3756issn
1791-244Xjournal_volume
13pub_type
杂志文章abstract：:Parkinson's disease (PD) is a common age-associated neurodegenerative disorder. To date, stem cell transplantation therapy has been developed to replace lost or damaged neural cells in PD patients, in whom dopaminergic neuron cells are lost. Here, we show that CD44+ human amniotic fluid cells (HuAFCs) can be induced t...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2011.732
更新日期：2011-11-01 00:00:00
abstract：:GINS2 has been found to be closely related to cell proliferation, survival and maintenance of genomic integrity in addition to the induction of polyploidy. However, the molecular mechanisms of GINS2-mediated tumor regression have not been fully elucidated. Our study showed that GINS2 interacted with PML-C (a structura...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2013.1339
更新日期：2013-06-01 00:00:00
abstract：:betaA25-35, a neurotoxic fragment of the Alzheimer beta-amyloid peptide (betaA), acts as a strong inducer of pro-inflammatory cytokines, such as interleukin (IL)-1 and IL-6, in glial cells. Since IL-1 is known to induce expression of both IL-1 and IL-6, we have investigated to what extent the induction of IL-1alpha an...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.1.1.201
更新日期：1998-01-01 00:00:00
abstract：:Oxygen-related free radicals have been suggested as a cause of aging and various diseases, for example, various cancers and rheumatoid arthritis. Because of this a radical scavenger as an antioxidant has been sought in many foods. 'Nikogori' gelatin gel from cooked fish is a traditional food in Japan. Recently, 'Nikog...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2005-09-01 00:00:00
abstract：:We had shown previously that the novel, marine, anticancer compound dehydrothyrsiferol (DHT) does not modulate P-glycoprotein (P-gp) dependent drug efflux. Many chemotherapeutics with clinical impact are substrates for the structurally distant related membrane transport protein MRP1 (multidrug resistance-associated pr...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：:Type 2 cytokine‑associated immunity may be involved in the pathogenesis of allergic asthma. Although interleukin 27 (IL‑27) has been reported as an initiator and suppressor of T‑helper 1 (Th1) and T‑helper 2 (Th2) responses, respectively, its effects on the development of asthma remain unclear. In the present study, m...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2020.4622
更新日期：2020-08-01 00:00:00
abstract：:Alzheimer's disease (AD) is a common neurodegenerative disorder characterized by aberrant tau protein hyperphosphorylation, which eventually leads to the formation of neurofibrillary tangles. Hyperphosphorylated tau protein is considered as a vital factor in the development of AD and is highly associated with cognitiv...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2017.3281
更新日期：2018-02-01 00:00:00
abstract：:We reported previously that a novel dipeptide alcohol, L-homoserylaminoethanol (Hse-Gly-ol), is a selective inhibitor of eukaryotic DNA polymerase epsilon (pol epsilon). The discovery suggests that the dipeptide structure could be a chemical frame for a DNA polymerase inhibitor. Therefore, we chemically synthesized 14...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2005-10-01 00:00:00
abstract：:Laminarin, found in marine brown algae, is used as a carbohydrate reserve for phytoplankton; however, it is also used in traditional Chinese medicine, and has been shown to have several biological activities, including anticancer activities. In this study, we examined the mechanisms through which laminarin from Lamina...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2013.1409
更新日期：2013-08-01 00:00:00
abstract：:In the wide horizon of ophthalmologically rare diseases among retinitis pigmentosa forms, Stargardt disease has gradually assumed a significant role due to its heterogeneity. In the present study, we aimed to support one of two opposite hypotheses concerning the causative or protective role of heterozygous c.1268A>G m...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2017.2917
更新日期：2017-04-01 00:00:00
abstract：:Alzheimer's disease (AD) is a chronic neurodegenerative disease that often occurs at a slow pace yet deteriorates with time. MicroRNAs (miRs) have been demonstrated to offer novel therapeutic hope for disease treatment. The aim of the present study was to investigate the effect of miR‑98 on amyloid β (Aβ)‑protein prod...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2018.3957
更新日期：2019-01-01 00:00:00
abstract：:A microarray analysis of an animal model with experimental sepsis induced by caecal ligation and puncture revealed that the level of microRNA‑195 (miR‑195) was upregulated. However, to the best of our knowledge, the role of miR‑195 in sepsis remains unknown. The present study investigated the effect of miR‑195 on apop...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2019.4431
更新日期：2020-02-01 00:00:00
abstract：:Members of the basic helix-loop-helix (bHLH) family of transcription factors have been implicated in the regulation of cell proliferation and differentiation. Here we describe molecular characterization of mesoderm-specific bHLH protein, POD-1/Capsulin. Northern blot analysis showed that POD-1/Capsulin are preferentia...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.5.1.27
更新日期：2000-01-01 00:00:00
abstract：:Rac3 is a small GTPase of the Rho family, members of which have been implicated in tumorigenesis, cell growth/death and organization of the actin cytoskeleton. Many Rac-interacting proteins or effectors identified to date have a role in cytoskeletal organization. Using the yeast two-hybrid system, we have isolated a n...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.1.4.665
更新日期：1998-04-01 00:00:00
abstract：:Smoking has deleterious effects on osteoporosis and periodontitis both characterized by bone loss. Smoking also interferes with the protective effect that hormone replacement therapy (HRT) has on bone loss. Our study investigated two mechanisms by which smoking may affect bone metabolism: nicotine-induced proliferatio...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:The prevalence of psoriasis is increasing, and poses a serious risk to human health and quality of life. Psoriasis, a chronic immune‑mediated skin disease with epidermal hyperkeratosis and parakeratosis, is associated with numerous complications, including metabolic syndromes that are regulated by sirtuins (SIRTs) via...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2019.4173
更新日期：2019-07-01 00:00:00
abstract：:The prognosis of patients suffering from glioblastoma [also referred to as glioblastoma multiforme (GBM)] is dismal despite multimodal therapy. Chemotherapy with temozolomide may suppress tumor growth for a certain period of time (a few months); however, invariable tumor recurrence suggests that glioblastoma initiatin...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2017.3124
更新日期：2017-11-01 00:00:00
abstract：:Chronic asthma is characterized by inflammatory cell infiltration and tissue remodeling, leading to subepithelial inflammation. In order to evaluate the anti-asthmatic activity of LX519290, a derivative of L-allo threonine, we performed several in vitro and in vivo anti-asthmatic assays. Using ovalbumin (OVA)-sensitiz...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2013.1265
更新日期：2013-04-01 00:00:00
abstract：:Breast cancer accounts for 30-40% of all deaths from cancers in females. In an effort to identify tumor associated antigens that may be useful for immunotherapy, we utilized serological analysis of recombinant cDNA expression libraries (SEREX) technique to identify breast cancer-associated antigens. SEREX screening of...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:Platinum compounds induce their cytotoxic effect by binding to a DNA molecule in the form of a platinum-DNA-adduct. Many previous studies have shown that the level of platinum-DNA-adduct correlats with response to platinum-based chemotherapy. Although the mechanism of platinum resistance in vivo is not clearly underst...
journal_title：International journal of molecular medicine
pub_type： 杂志文章,评审
doi：
更新日期：2004-12-01 00:00:00
abstract：:Regucalcin is a Ca2+-binding protein which plays a regulatory role in liver cell functions related to Ca2+. In this study we have cloned and characterized cDNA for regucalcin from human liver and human hepatoma cell line Hep G2 by screening and rapid amplification of cDNA ends (RACE). The nucleotide sequences of the c...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:Human histocompatibility leukocyte antigen (HLA)-G is a nonclassical major histocompatibility complex class I molecule. HLA-G is known to provide tolerance from recognition by natural killer cells. We studied HLA-G expression in 39 human colorectal cancers and 23 extra-neoplastic colon tissue samples by RT-PCR. The ex...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2.3.349
更新日期：1998-09-01 00:00:00
abstract：:The purpose of this study was to investigate the expression of different CD44 and hyaluronan synthase isoforms in cartilage, their alterations during the chondrocyte dedifferentiation process in monolayer culture and during the redifferentiation process on 3D scaffolds. Chondrocytes isolated from human articular carti...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2009-02-01 00:00:00
abstract：:Human bladder exstrophy-epispadias complex (BEEC) comprises a spectrum of urogenital anomalies in which part or all of the distal urinary tract fails to close. Several lines of evidence implicate genetic factors in the formation of BEEC. Among them a murine p63+/+ knockout model showed the full picture of classic exst...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm_00000535
更新日期：2010-12-01 00:00:00
abstract：:Peripheral arterial disease (PAD) affects about 5% of the elderly population in the western world. It has been reported that PAD is associated with elevated plasma levels of the inflammatory markers. The goal of this review is to describe which proinflammatory molecules may play a role in PAD development, C-reactive p...
journal_title：International journal of molecular medicine
pub_type： 杂志文章,评审
doi：
更新日期：2007-09-01 00:00:00
abstract：:It is known that cancers develop by a multi-step process. Normal cells are first immortalized, and then transformed into tumorigenic cells. Normal human cells are very rarely immortalized, but once they are, they are relatively easily transformed into tumorigenic cells. This indicates that the immortalization step pla...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2.5.603
更新日期：1998-11-01 00:00:00
abstract：:In recent studies, we and others have demonstrated that bone morphogenetic protein-2 (BMP-2) promotes vascularization, inhibits hypoxic cell death of cancer cells and may be involved in tumor angiogenesis. The activation of circulating endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) represents a ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.18.4.735
更新日期：2006-10-01 00:00:00
abstract：:A variety of microRNAs (miRNAs) have been reported to be significantly be involved in the regulation of vascular smooth muscle cell (VSMC) proliferation, which is an essential process for the formation of atherosclerotic plaque. The objective of the present study was to explore the role of microRNA-155 (miR-155) in th...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2015.2181
更新日期：2015-06-01 00:00:00
abstract：:A large number of p53-transcribed proteins have been shown to mediate growth arrest and/or apoptosis in vitro, whereas their in vivo roles remain largely unclear. p53 is capable of initiating apoptosis without transcription of apoptosis inducer genes, although the underlying mechanism is unknown. p53 is present in the...
journal_title：International journal of molecular medicine
pub_type： 杂志文章,评审
doi：
更新日期：2002-01-01 00:00:00
abstract：:The spindle checkpoint proteins (SCPs), which sense the existence of misaligned sister chromatids during mitosis and meiosis, are essential for cell proliferation and differentiation. Therefore, the role of SCPs in carcinogenesis is gaining increased attention. In this study, we ana...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2012.1168
更新日期：2013-01-01 00:00:00
