Abstract:
:Photodynamic therapy (PDT) is an evolving cancer treatment with promising results in treating malignant tumors by photoactivation of a photosensitizer with a specific wavelength. The second generation photosensitizer mono-L-aspartyl chlorin e6 (NPe6) was reported to have significant efficacy in killing cancer cells in vitro and in vivo. Though topical application might yield a higher local concentration and less systemic side effect, no data concerning topical absorption of NPe6 is available even though the drug has already been used in clinical trial for several years. To evaluate the possibility of topical delivery of NPe6 via an animal model, escalated concentrations of NPe6 were applied to BALB/c mouse skin for a different time periods after barrier disruption with tape stripping. Since NPe6 fluorescence intensity and drug concentration in tissue was well correlated, we evaluated drug penetration depth with frozen sections of treated and non-treated skin under a fluorescence microscope. An on-line fluorescence imaging system was used to monitor the NPe6 fluorescence kinetics in the skin. The fluorescence microscope confirmed successful topical delivery of NPe6 in mouse skin with or even without barrier disruption. Orange to red NPe6 fluorescence appeared at the epidermis, dermis, and even the muscular layer when using 10 mg/ml NPe6 application. The fluorescence intensity peaked at 1 h and revealed a dose-dependent response pattern. NPe6 treated versus non-treated skin showed a statistically significant difference by Student's t-test (P<0.05). The results described here suggest that topical delivery of NPe6 is possible. It showed fast and deep penetration into mouse skin. This implies that NPe6 might be useful as a topical photosensitizer for PDT in treating skin cancers.
journal_name
J Pharmacol Scijournal_title
Journal of pharmacological sciencesauthors
Wong TW,Aizawa K,Sheyhedin I,Wushur C,Kato Hdoi
10.1254/jphs.93.136subject
Has Abstractpub_date
2003-10-01 00:00:00pages
136-42issue
2eissn
1347-8613issn
1347-8648journal_volume
93pub_type
杂志文章abstract::In the present study, we investigated the effect of aripiprazole, a dopamine system stabilizer, on ethanol-induced psychological and physiological dependence and anxiety-like behavior. First we determined the effect of aripiprazole, a dopamine system stabilizer, on the development and expression of ethanol-induced pla...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.11201fp
更新日期:2012-01-01 00:00:00
abstract::Multi-glycoside, one of the extracted compounds from Tripterygium wilfordii HOOK. f. (GTW), has been shown to be clinically effective in suppressing glomerular inflammation in chronic kidney disease. However, its clinical application is often limited by its cytotoxic actions on the liver. This study was performed to c...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.11157fp
更新日期:2012-01-01 00:00:00
abstract::We studied the pronociceptive role of proteinase-activated receptor-2 (PAR2) in mouse bladder. In female mice, intravesical infusion of the PAR2-activating peptide, SLIGRL-amide (SL), caused delayed mechanical hypersensitivity in the lower abdomen, namely 'referred hyperalgesia', 6-24 h after the administration. The P...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2017.12.007
更新日期:2018-01-01 00:00:00
abstract::We recently developed a novel cognitive enhancer, ST101 (spiro[imidazo[1,2-a]pyridine-3,2-indan]-2(3H)-one), that activates T-type voltage-gated calcium channels (VGCCs). Here, we address whether T-type VGCC activation with ST101 mediates its cognitive effects in vivo and the relevance of T-type VGCC activation to ace...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.12233fp
更新日期:2013-01-01 00:00:00
abstract::Prolonged QT interval is an independent risk factor for development of ventricular arrhythmias. Haloperidol is one of the drugs inducing QT prolongation. Previous studies showed that haloperidol affects not only QT duration but also heart rate (RR interval). The present work focused on relationship between QT and RR a...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2018.11.004
更新日期:2019-01-01 00:00:00
abstract::Resveratrol is a stilbene compound found in grapes and other sources. In this study we examined the effects of trans-resveratrol (4.38 - 438 microM/implant) in the vasculogenesis of yolk-sac membranes and its capacity to improve chick embryo growth. High concentrations of the stilbene (43.8 - 438 microM) significantly...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.fp0071876
更新日期:2008-06-01 00:00:00
abstract::N-methyl-N-nitrosourea (MNU) is widely used to study the mechanism of retinal degenerative diseases (RDs) because of its selectivity of photoreceptor cell death. Many reports suggest that excessive nitric oxide (NO) plays a crucial role in neuronal cell death. We hypothesized that nitric oxide synthase (NOS)/NO are in...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2015.02.008
更新日期:2015-03-01 00:00:00
abstract::The effect of T-2328 {2-fluoro-4'-methoxy-3'-[[[(2S,3S)-2-phenyl-3-piperidinyl]amino]methyl]-[1,1'-biphenyl]-4-carbonitrile dihydrochloride}, a novel tachykinin NK(1)-receptor antagonist, was examined on cisplatin-induced emesis in ferrets. Cisplatin induced acute emesis in 24 h and delayed emesis during 24 and 72 h, ...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.08027fp
更新日期:2008-06-01 00:00:00
abstract::Schisandra chinensis is a hepatoprotective herb that has been used for centuries in China. Polysaccharide is one of the major active components in S. chinensis, which has been reported to improve liver injuries induced by carbon tetrachloride, alcohol, or high-fat diet. In this study, we observed the effects and corre...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2019.07.008
更新日期:2019-07-01 00:00:00
abstract::There are several animal models of glucocorticoid-induced osteoporosis (GIOP), but each requires a long time to evaluate drug effects. Zebrafish scales are classified as dermal bone and potentially represent a convenient animal model of GIOP because they rapidly regenerate following their removal. We clarified that de...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2020.02.011
更新日期:2020-06-01 00:00:00
abstract::The optimum strategy for heart failure (HF) treatment has yet to be elucidated. This study intended to test the benefit of a combination of valsartan (VAL) and perifosine (PER), a specific AKT inhibitor, in protecting against pressure overload induced mouse HF. Mouse were subjected to aortic banding (AB) surgery to es...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2020.04.001
更新日期:2020-07-01 00:00:00
abstract::In the present study, we investigated the protective effect of methyl 3,4-dihydroxybenzoate (MDHB) against H2O2-induced apoptosis in RGC-5 cells. The RGC-5 cells were cultured in plates for 24 h, which were then pretreated with dimethyl sulfoxide, different concentrations of MDHB, or probucol for 12 h prior to additio...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.13055fp
更新日期:2014-01-01 00:00:00
abstract::The prevalence rate of cardiovascular disease is higher for males than females, and estradiol (E2) induces AMP-activated protein kinase (AMPK) activation, which is known to regulate proliferation of VSMC. We identified the estrogenic properties of nordihydroguaiaretic acid (NDGA, a lignan phytoestrogen) that inhibit V...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2016.09.001
更新日期:2016-09-01 00:00:00
abstract::Serotonin/noradrenaline reuptake inhibitors (SNRIs) are widely used for the treatment for major depressive disorder, but these drugs induce several side effects including increased aggression and impulsivity, which are risk factors for substance abuse, criminal involvement, and suicide. To address this issue, milnacip...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2017.06.004
更新日期:2017-07-01 00:00:00
abstract::Aspirin (acetylsalicylic acid) is a well-known nonsteroidal anti-inflammatory drug that can potentiate some acute allergies and causes adverse immunological reactions collectively referred to as aspirin intolerance, a disorder that induces urticaria, asthma, and anaphylaxis in response to oral administration of the dr...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章,评审
doi:10.1254/jphs.08r32fm
更新日期:2009-07-01 00:00:00
abstract::The critical role played by the renin-angiotensin-aldosterone system (RAAS) in the regulation of blood pressure and body fluid homeostasis has been well recognized. Angiotensin (Ang) II and aldosterone are the most powerful biologically active products of the RAAS, although there are also other bioactive Ang peptides ...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章,评审
doi:10.1254/jphs.10r03fm
更新日期:2010-01-01 00:00:00
abstract::Metformin has received increasing attention for its potential anticancer activity against certain human leukemia cells, but its effects on human megakaryoblastic cells are unclear. This study aimed to investigate the effects of metformin on proliferation and apoptosis of human megakaryoblastic cells (Dami and MEG-01) ...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2017.08.003
更新日期:2017-09-01 00:00:00
abstract::Antipsychotics are often used in conjunction with anti-Alzheimer drugs to treat the behavioral and psychological symptoms of dementia (BPSD). Here, we examined the effects of cholinesterase inhibitors (ChEIs), donepezil and galantamine, on antipsychotic-induced extrapyramidal side effects (EPS) in mice. The effects of...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2015.03.004
更新日期:2015-04-01 00:00:00
abstract::We aimed to examine the rate of thrombotic events after discontinuation of one year clopidogrel therapy in patients with implanted coronary stent, and to determine platelet aggregability by multiple electrode analyzer after cessation of clopidogrel. This prospective, multicenter study enrolled 200 patients subjected t...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.11076fp
更新日期:2011-01-01 00:00:00
abstract::Although mannitol has been used as an osmotherapeutic drug on brain injury, the clinical efficiency of the drug are still controversial. In the present study, we examined the effects of mannitol on the edema in a hippocampal slice due to brief ischemia. To evaluate the effects, we employed an image analysis system tha...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.fp0030565
更新日期:2004-07-01 00:00:00
abstract::In the novel object recognition task, ZSET1446 (also coded as ST101) enhanced object recognition memory in mice and ameliorated cognitive impairment caused by scopolamine in rats. The enhancement induced by ZSET1446 in mice was abolished by injection of mechamylamine, a nonselective antagonist of nicotinic acetylcholi...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.13042fp
更新日期:2013-01-01 00:00:00
abstract::We investigated whether endothelin (ET) would be involved in skin cancer pain in mice. Orthotopic inoculation of B16-BL6 melanoma cells into the plantar region of the hind paw produced marked mechanical allodynia in C57BL/6 mice. Intraplantar injections of the ET(A)-receptor antagonist BQ-123 (0.3 - 3 nmol/site), but ...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.fp0072051
更新日期:2008-02-01 00:00:00
abstract::Although cyclic ADP-ribose (cADPR), a novel Ca(2+)-mobilizing mediator, is suggested to be involved in the functions of neutrophils in rodents, its role in human neutrophils remains unclear. The present study examined the ability of cADPR to mobilize Ca(2+) and mediate formyl methionyl leucyl phenylalanine (fMLP)-stim...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.fp0072083
更新日期:2008-03-01 00:00:00
abstract::Melittin is a major peptide component of sweet bee venom that possesses anti-allergic, anti-inflammatory, anti-arthritis, anti-cancer, and neuroprotective properties. However, the therapeutic effects of melittin on muscle injury have not been elucidated. We investigated the therapeutic effects of melittin on muscle in...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2019.03.009
更新日期:2019-05-01 00:00:00
abstract::In the present study, we investigated whether a novel benzopyranylindol analogue, KR-31466 (KR466) (1-[(2S,3R,4S)-3,4-dihydro-2-dimethoxymethyl-3-hydroxy-2-methyl-6-nitro-2H-1-benzopyran-4-yl]-1H-indole-2-carboxylic acid ethyl ester) can attenuate hypoxic injury in heart-derived H9c2 cells and, if so, whether the prot...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.92.13
更新日期:2003-05-01 00:00:00
abstract::We have demonstrated previously that the ketamine-induced discriminative stimulus effect is likely to reflect the phencyclidine-like psychotomimetic effects. Therefore, the present study was designed to investigate the effects of the antipsychotics and 5-HT2 receptor antagonist on the discriminative stimulus effects o...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.12148sc
更新日期:2013-01-01 00:00:00
abstract::The beneficial effects of physical activity for pain are denominated exercise-induced hypoalgesia (EIH). Here, we examined the age-related change and potential role of the neurosteroid allopregnanolone (ALLO) on EIH in rats. Adult and aged rats were randomly divided into one of three groups; non-exercise control, Low-...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2018.11.009
更新日期:2019-02-01 00:00:00
abstract::We have previously shown a marked but transient increase in DNA binding of the nuclear transcription factor activator protein-1 after brief exposure to static magnetic fields in cultured rat hippocampal neurons, suggesting that exposure to static magnetism would lead to long-term consolidation as well as amplification...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章,评审
doi:10.1254/jphs.fmj05001x5
更新日期:2005-07-01 00:00:00
abstract::Metabolic syndrome (MetS) induces serious complications; therefore, we developed a noninvasive MetS model using an extremely small minipig, the Microminipig. For 8 weeks, Microminipigs were administrated a high-fat and high-cholesterol diet (HFCD) for atherosclerosis and N(G)-nitro-l-arginine methyl ester (l-NAME) for...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.14171sc
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:Treatment with the chemotherapeutic agent, doxorubicin (DOX), is limited by side effects. We have previously demonstrated that fasudil, a Rho/ROCK inhibitor, has antioxidant, anti-inflammatory and anti-apoptotic effects in contrast-induced acute kidney injury model. The present study to investigated the possi...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2020.10.002
更新日期:2021-01-01 00:00:00