Abstract:
BACKGROUND:Tissue polypeptide antigen (TPA) is a circulating complex of polypeptide fragments from cytokeratins 8, 18 and 19. It is a tumour-related protein. TPA is an indicator of higher cell proliferation. One function of TP53 is the suppression of apoptosis. TP53 mutations are frequently correlated with tumour development in bladder cancer. One function of TP53 is the suppression of apoptosis. We compared TPA expression and TP53 mutation analysis in tumour-free and bladder cancer patients. MATERIALS AND METHODS:We examined 93 patients with bladder cancer, 24 patients with benign urological diseases and a control group of 18 healthy individuals. TPA concentration was measured by immunoluminometric assay with LIA-mat TPA-M Prolifigen. The normal cut-off value was defined at 47 U/I for serum and at 60 U/mmol for creatinine. Screening for TP53 mutations in tissue and urine sediment, amplification of the TP53 gene by polymerase chain reaction (PCR) for the exons 5, 6, 7 and 8 and temperature gradient gel electrophoresis (TGGE) were used to analyse the mutations. Statistical analysis included ROC, Mann-Whitney U-Test and Pearson's correlation. RESULTS:For superficial bladder cancer the mutation frequency in TP53 was 44.8%. We found elevated TPA levels in 45.5% in serum and 36.1% in urine. For invasive bladder cancer the mutation frequency in TP53 was 79.2%. Elevated TPA levels were found in 57.7% in serum and in 58.3% in urine. TPA has a sensitivity of 48.9% in serum and 40.4% in urine; the specificity of TPA is 83% in serum and 100% in urine in comparison with healthy individuals. We found no correlation between TPA level and the inflammation status of the patient. CONCLUSION:This study demonstrated that TP53 mutation frequently occurs in higher stages of bladder tumours. There was no TPA level difference between superficial and invasive bladder cancer. TPA is significantly higher in serum (p = 0.012) and in urine (p = 0.002) in patients with bladder cancer in comparison with control group. TPA in serum is significantly higher in patients with mutation of TP53 (p = 0.046) but not in urine (p = 0.173) in comparison with patients with wild-type TP53.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Ecke TH,Lenk SV,Schlechte HH,Loening SAsubject
Has Abstractpub_date
2003-03-01 00:00:00pages
957-62issue
2Aeissn
0250-7005issn
1791-7530journal_volume
23pub_type
杂志文章abstract:BACKGROUND/AIM:Since the first description of five pericytomas with the t(7;12)/ACTB-GLI1 fusion gene, only three new tumors were studied by both cytogenetics and molecular techniques. We report here genetic data on another case of this rare tumor. MATERIALS AND METHODS:Cytogenetic, fluorescence in situ hybridization ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.14065
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2011-02-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2019-08-01 00:00:00
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journal_title:Anticancer research
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更新日期:2000-09-01 00:00:00
abstract:BACKGROUND:The efficacy of positron emission tomography with 18F fluoro-2-deoxy-D-glucose (FDG-PET) is obscure in evaluating viability or the extent of colorectal hepatic metastasis (CHM), down-staged by chemotherapy. PATIENTS AND METHODS:A retrospective lesion-by-lesion analysis was performed for seven consecutive pa...
journal_title:Anticancer research
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doi:
更新日期:2006-11-01 00:00:00
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journal_title:Anticancer research
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doi:
更新日期:2000-09-01 00:00:00
abstract::Melanoma is a tumor of continuously increasing incidence for which new methods of imaging and targeted therapy are widely sought. Radioiodinated methylene blue is a promising tracer, showing selective uptake in human pigmented melanoma cells. We performed 131I-labeling of the tracer using 1% methylene blue injection U...
journal_title:Anticancer research
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更新日期:2008-11-01 00:00:00
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journal_title:Anticancer research
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doi:
更新日期:2004-03-01 00:00:00
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更新日期:2001-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2011-11-01 00:00:00
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journal_title:Anticancer research
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更新日期:1983-03-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2002-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2013-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1986-11-01 00:00:00
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doi:
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更新日期:2005-03-01 00:00:00
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journal_title:Anticancer research
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更新日期:1984-11-01 00:00:00
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pub_type: 杂志文章
doi:
更新日期:2002-11-01 00:00:00
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更新日期:1996-03-01 00:00:00
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更新日期:2018-11-01 00:00:00
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doi:
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