Abstract：
OBJECTIVE:Nontypeable Haemophilus influenzae (NTHi) is one of the leading causative pathogens for otitis media. The outer membrane protein P6 of NTHi is highly conserved among the strains and is an attractive candidate for a preventive vaccine. However, for the production of a relatively small amount P6 containing lipopolysaccharides, the development of a recombinant version of this protein is required. This study was designed to investigate the specific mucosal immunity induced by intranasal immunization of recombinant P6 (rP6) with cholera toxin (CT). METHODS:BALB/c mice were immunized with of rP6 (30 microg) and CT (2 microg) intranasally every 2 days for 2 weeks. Anti-rP6 specific IgG, IgA and IgM antibodies and the subclass of anti-rP6 specific IgG antibody were determined by enzyme linked immunosorbent assay (ELISA). Anti-rP6 specific IgA in nasopharyngeal washings were also determined by ELISA. Nasopharyngeal clearance of inoculated NTHi after the intranasal immunization were assessed. All statistical differences between the two groups were assessed by ANOVA parametric test. RESULTS:Intranasal immunization with rP6 and CT evoked rP6-specific mucosal IgA immune response as well as the systemic IgG immune response against rP6 and enhanced nasopharyngeal clearance of inoculated live NTHi. CONCLUSION:These results indicate the good immunogenicities of rP6 to induce specific immune responses against NTHi. Intranasal immunization with rP6 will be an effective approach to protect infections of NTHi.
journal_name
Int J Pediatr Otorhinolaryngoljournal_title
International journal of pediatric otorhinolaryngologyauthors
Hotomi M,Yamanaka N,Shimada J,Suzumoto M,Ikeda Y,Sakai A,Arai J,Green Bdoi
10.1016/s0165-5876(02)00076-9subject
Has Abstractpub_date
2002-09-02 00:00:00pages
109-16issue
2eissn
0165-5876issn
1872-8464pii
S0165587602000769journal_volume
65pub_type
杂志文章abstract：:We present two siblings with severe laryngomalacia requiring surgical intervention during the newborn period, microcephaly, developmental delay, cleft palate, preaxial polydactyly, dysplastic nails and conductive hearing loss (persistent after tympanostomy tube placement). In addition the girl has microopthalmia and t...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章,评审
doi：10.1016/j.ijporl.2006.04.010
更新日期：2006-09-01 00:00:00
abstract：OBJECTIVES:Silymarin is a plant extract with strong antioxidant properties in addition to anti-inflammatory and anticarcinogenic actions. The aim of this study was to investigate the potential preventive effect of silymarin on cisplatin ototoxicity in an auditory cell line, HEI-OC1 cells. METHODS:Cultured HEI-OC1 cell...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2013.12.024
更新日期：2014-03-01 00:00:00
abstract：OBJECTIVES:Adenotonsillar hypertrophy is the major determinants of habitual snoring in pediatric population. Behavioral hyperactivity and schooling problems have been repeatedly reported in these children, and it may underlie more extensive behavioral disturbances, particularly for the obese children. The aim of the pr...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2014.09.026
更新日期：2014-12-01 00:00:00
abstract：:The pattern of referral of infants and children with tracheotomy to speech-language pathology (SLP) was studied through a retrospective review. Less than half (29/62) were referred to SLP with no difference by surgical service (otolaryngology vs pediatric surgery) or length of time with tracheotomy. Younger children w...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/0165-5876(92)90105-x
更新日期：1992-05-01 00:00:00
abstract：OBJECTIVE:Acute otitis media (AOM) is one of the most common infectious diseases in children. Nontypeable Haemophilus influenzae (NTHi) is Gram-negative bacteria that are considered major pathogens of AOM and respiratory tract infections. In this study, we used monophosphoryl lipid A (MPL), a toll-like receptor (TLR) 4...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2017.03.018
更新日期：2017-06-01 00:00:00
abstract：:Assessment of candidacy for cochlear implantation in children continues to present a challenge to cochlear implant programs. The efficacy of implantation depends on a number of factors and as a result a multidisciplinary approach has been adopted. At the Cochlear Implant Program at The Hospital for Sick Children, Toro...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/s0165-5876(99)00112-3
更新日期：1999-08-05 00:00:00
abstract：OBJECTIVE:To evaluate the relationship between neck abscess characteristics on computerized tomography (CT) scan and surgical drainage in pediatric patients. METHODS:Retrospective data warehouse review identified 43 children younger than 19 years admitted to a tertiary care pediatric hospital during the first quarters...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2007.10.016
更新日期：2008-02-01 00:00:00
abstract：OBJECTIVE:To identify the perception of the train passengers about the cause and the management of hearing loss. METHODOLOGY:A total of 115 passengers, 90 males and 25 females, in the age range of 13-80 years (mean: 39.19, standard deviation: 15.4) years. RESULTS:From the survey, it is found that most of the particip...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2012.04.014
更新日期：2012-08-01 00:00:00
abstract：:The treatment of infections in the ring of Waldeyer in children is an ever present problem. Most infections are caused by group A streptococci, and various antibiotics are routinely used for their treatment. In many cases, however, this treatment is ineffective as demonstrated by prolonged disease duration and recurre...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/s0165-5876(84)80053-1
更新日期：1984-03-01 00:00:00
abstract：:Humans are born 12 months too early. Gestation should be 21 months. Humans evolved to become the pre-eminent animal in the world, but our big brain, bipedalism, and small female pelvic outlet have caused us to pay the price of being born too soon with all of its disadvantages. Early birth has an impact on diseases and...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章,评审
doi：10.1016/j.ijporl.2004.07.021
更新日期：2005-01-01 00:00:00
abstract：:In children, persistent upper airway obstruction may lead to increased pulmonary arterial pressure (PAP). Allergic rhinitis (AR) is one of the frequent cause of persisting upper airway obstruction by nasal blockage in childhood. Regular use of nasal topical corticosteroids are effective in reducing nasal blockage and ...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/s0165-5876(01)00500-6
更新日期：2001-07-30 00:00:00
abstract：OBJECTIVES:Etiological diagnosis of hearing impairment is of great importance to ensure early and adequate management. Even after thorough history taking, clinical and audiometric evaluation, the cause of hearing loss remains unclear in a majority of patients. Further examinations can imply imaging, ophthalmologic inve...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2014.12.012
更新日期：2015-02-01 00:00:00
abstract：INTRODUCTION:The use of honey in wound care is becoming more common due to the proven benefit in all three phases of wound healing, as well as the antibacterial and antibiofilm properties. We present our experience using TheraHoney gel, a medical grade honey, for the successful treatment of cochlear implant associated ...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2018.05.026
更新日期：2018-08-01 00:00:00
abstract：:Although the incidence of Obstructive Sleep Apnea syndrome (OSAS) in craniofacial syndromes is high, it is often not recognized and thus not treated. In order to study the diagnostics and treatment options for these patients, we studied a group of 72 patients treated in our hospital for Apert, Crouzon, or Pfeiffer syn...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2003.08.007
更新日期：2003-12-01 00:00:00
abstract：BACKGROUND:Prosody is the aspect of language that conveys emotion by changes in tone, rhythm, and emphasis during speech and the term specific language impairment (SLI) refers to children whose language development is substantially below their chronological age, despite a normal nonverbal intelligence and no obvious ne...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2014.11.001
更新日期：2015-01-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to investigate the status of matrix metalloproteinases (MMP-2, MMP-7, MMP-9) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) due to dysregulated turnover of connective tissue matrices in children with recurrent tonsillitis (RT). METHODS:Forty-four patients with RT were e...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2010.02.016
更新日期：2010-05-01 00:00:00
abstract：OBJECTIVE:In this study, we developed a biocompatible and biodegradable vancomycin (VM)-eluting electrospun poly(ɛ-caprolactone) (PCL)/polyethylene oxide (PEO)/VM/PCL nanofiber mat. The purpose of this study was to evaluate the efficacy of this nanofiber mat for preventing methicillin-resistant Staphylococcus aureus (M...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2015.05.037
更新日期：2015-08-01 00:00:00
abstract：OBJECTIVES:The aims of the present study were to evaluate the efficacy of and compliance with a new device for autoinflation in the treatment of persistent otitis media with effusion (OME) in young children. METHODS:Forty-five children with persistent OME with a bilateral type B or C2 tympanogram for at least three mo...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.ijporl.2014.05.015
更新日期：2014-08-01 00:00:00
abstract：AIM:The main aim of this study was to explore factors compromising early intervention (EI) service delivery to hearing impaired children in South Africa, as expressed by their caregivers. METHODS:Within a qualitative survey design, a sample of 19 hearing impaired children's caregivers completed structured self-adminis...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2018.12.021
更新日期：2019-03-01 00:00:00
abstract：OBJECTIVE:Recurrent respiratory papillomatosis (RRP) is a benign aerodigestive tract neoplasm. Cidofovir, an antiviral drug, has demonstrated efficacy in slowing and/or reducing RRP recurrence. This investigation examined the differential gene expression of RPP before and after cidofovir use in vivo. METHODS:Papilloma...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2008.06.001
更新日期：2008-09-01 00:00:00
abstract：OBJECTIVES:This study was conducted to analyze the association between recent antimicrobial use and acute otitis media (AOM) due to Streptococcus pneumoniae intermediately resistant to penicillin (PISP). The influence of drug resistance on the clinical course of AOM was also assessed. METHODS:A retrospective cohort st...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2009.06.007
更新日期：2009-10-01 00:00:00
abstract：:Treatment of nasal septal deformity in childhood has received growing acceptance in recent years. Traditionally, concern about the role of the septum in the overall growth of the midface has led otolaryngologists to take a very cautious approach to correction of septal deformities in children. However, a great deal of...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章,评审
doi：10.1016/0165-5876(95)01306-7
更新日期：1996-05-01 00:00:00
abstract：OBJECTIVE:To assess the link between sleep-disordered breathing and cognitive function in children. To identify correlations among polysomnography, upper respiratory infections, or cephalometric as well as rhinometric measures. METHODS:This study is based on a questionnaire survey of snoring in a population cohort of ...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2011.12.002
更新日期：2012-03-01 00:00:00
abstract：OBJECTIVE:To assess the impact of the waiting list on the spontaneous resolution of recurrent acute tonsillitis in children. METHODS:We have evaluated 623 cases placed on the waiting list for elective tonsillectomy (with or without adenoidectomy) between February 1994 and May 1999 at our institution. In each child, ag...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/s0165-5876(02)00128-3
更新日期：2002-08-01 00:00:00
abstract：OBJECTIVES:The main objective was to examine the effect of central maturation on the auditory temporal resolution in a group of school-age children using Gaps-In-Noise test. METHODS:The study involved 180 children (6-16 years) with normal hearing, average intelligence and language skills, and adequate scholastic achie...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2019.04.010
更新日期：2019-07-01 00:00:00
abstract：INTRODUCTION:Dexmedetomidine is a novel pharmacologic agent that has become a frequently used adjunct during care of pediatric patients with obstructive sleep apnea (OSA) undergoing tonsillectomy. While generally safe and effective, dexmedetomidine is associated with adverse effects of hypotension and bradycardia from ...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2017.11.025
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND:Studies related to plasticity and learning-related phenomena have primarily focused on higher-order processes of the auditory system, such as those in the auditory cortex and limited information is available on learning- and plasticity-related processes in the auditory brainstem. DESIGN AND METHOD:A clinica...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2012.12.032
更新日期：2013-04-01 00:00:00
abstract：OBJECTIVE:The purpose of this study is to compare the levels of depressive emotioning of adolescents with cochlear implants and the ones who have normal hearing. METHODS:For this purpose, Rosenberg Self-Esteem Scale is applied upon the study group which consists of 30 adolescents with cochlear implant between the ages...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2009.09.027
更新日期：2009-12-01 00:00:00
abstract：OBJECTIVE:To implement a surgeon led initiative for tracheostomy education for non-surgical pediatric first responders in the hospital setting and to assess its efficacy. METHODS:A prospective, observational study conducted from September 2018 to March 2019. All pediatric residents at a tertiary care children's hospit...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2020.109944
更新日期：2020-06-01 00:00:00
abstract：UNLABELLED:Optimal management of deep neck abscesses has been the subject of debate for more than a century: surgical drainage has been the mainstay of treatment, but recently many centres have reported successful non-operative management in selected cases. OBJECTIVES:Our objective was to review the management of deep...
journal_title：International journal of pediatric otorhinolaryngology
pub_type： 杂志文章
doi：10.1016/j.ijporl.2012.09.006
更新日期：2012-12-01 00:00:00
