The effect of toluidine blue on the survival, dormancy and outer membrane porin proteins (OmpC and OmpF) of Salmonella typhimurium LT2 in seawater.

Abstract:

AIMS:To study the relationship between changes in the composition of the outer membrane proteins and the survival of Salmonella typhimurium LT2 in filtered autoclaved seawater containing Toluidine Blue (TB) dye as a photosensitizer. METHODS AND RESULTS:In samples exposed to TB and excited by artificial visible light, the total viable (TVC) and respiring cell counts (RCC) showed that, although the TVC declined to an undetectable level in 6.5 h, the RCC showed that some cells were still capable of respiration. The porin protein composition changed gradually with OmpC and OmpF becoming undetectable by sodium dodecyl sulphate-polyacrylamide gel electrophoresis after 8 h of incubation. Hydrogen peroxide-pretreated cells survived longer compared with the control. CONCLUSIONS:Oxidative pretreatment of Salm. typhimurium protects cells from some of the effects of sunlight in the presence of photosensitizers. The changes in porin proteins may play a role in this protection. SIGNIFICANCE AND IMPACT OF THE STUDY:The study shows that the survival of bacteria under conditions of stress is the result of a linked series of reactions.

journal_name

J Appl Microbiol

authors

Ozkanca R,Sahin N,Isik K,Kariptas E,Flint KP

doi

10.1046/j.1365-2672.2002.01642.x

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

1097-104

issue

6

eissn

1364-5072

issn

1365-2672

pii

1642

journal_volume

92

pub_type

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