Abstract：
:A cyst-forming strain of Toxoplasma gondii was transfected with the Escherichia coli LacZ gene and expressed beta-galactosidase constitutively. This strain has been used to localize and analyze the early stages of development and reactivation of T. gondii in mice. The chromogenic detection of the enzyme allows an easy detection of the parasites after light fixation and therefore allows a submacroscopic analysis of tissue distribution within the organism. Also, it allows further embedding and retrieval of rare stages for electron microscopic observation. that detect the presence of the parasite and initiate the response, and (2) the early stages of reactivation, when the cysts are supposed to break open and release the infectious bradyzoïtes. We have taken advantage of the possibility of detecting the enzymatic activity of beta-galactosidase (beta-gal) in transfected parasites to show that one could perform a semi-macroscopic detection and that this was compatible with further analysis by histological or electron microscopic techniques, being therefore able to detect the rare events and then to analyze them further with more refined morphological techniques.
journal_name
Parasitol Resjournal_title
Parasitology researchauthors
Dao A,Soête M,Sergent V,Deslée D,Fortier B,Dubremetz JFdoi
10.1007/s004360100499subject
Has Abstractpub_date
2002-01-01 00:00:00pages
69-72issue
1eissn
0932-0113issn
1432-1955journal_volume
88pub_type
杂志文章abstract：:The history of European bison Bison bonasus Linnaeus, 1758 has been stormy since its extinction in the wild after the First World War. Due to the fact that the species was restored from just 12 founders, further expansion has suffered from low genetic variability, rendering the bison vulnerable to various pathogens du...
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pub_type： 杂志文章
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journal_title：Parasitology research
pub_type： 杂志文章
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journal_title：Parasitology research
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更新日期：2019-02-01 00:00:00
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更新日期：2006-01-01 00:00:00
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journal_title：Parasitology research
pub_type： 杂志文章,已发布勘误
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更新日期：2018-04-01 00:00:00
abstract：:Toxocara canis is an intestinal nematode of canids with a worldwide distribution, causing an important but neglected parasitic zoonosis in humans. Aquaporins (AQP) are a family of water channel proteins, which function as membrane channels to regulate water homeostasis. In this study, the coding sequence of aquaporin-...
journal_title：Parasitology research
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doi：10.1007/s00436-016-5129-8
更新日期：2016-09-01 00:00:00
