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Molecular characterization of a troponin I-like protein from the hard tick Haemaphysalis longicornis.

Abstract：

:A cDNA expression library prepared from mRNA of Haemaphysalis longicornis (H. longicornis) was screened with a H. longicornis-infested rabbit serum. A cDNA encoding 27/30kDa proteins was cloned and designated P27/30 gene. The predicted amino acid sequence of the P27/30 gene shows a rather high homology (58% amino acid identities and 11% amino acid similarity) with Drosophila melanogaster troponin I clone E2. H. longicornis P27/30 possesses amino acid sequence of actin-binding domains of troponin I at the amino acid residues 128-148, suggesting that H. longicornis P27/30 is a troponin I-like protein. By immunoblot analysis, mouse anti-recombinant P27/30 serum reacted with major constituent protein bands in extracts of adult ticks, and also immunoreacted with muscle, cuticle, gut, and salivary gland in H. longicornis ticks. Moreover, immunohistochemistry using the anti-P27/30 serum showed a strong reactivity in muscle, suggesting that native P27/30 is expressed abundantly in that tissue.

journal_name

Insect Biochem Mol Biol

journal_title

Insect biochemistry and molecular biology

authors

You M,Xuan X,Tsuji N,Kamio T,Igarashi I,Nagasawa H,Mikami T,Fujisaki K

doi

10.1016/s0965-1748(01)00081-9

subject

Has Abstract

pub_date

2001-12-01 00:00:00

pages

67-73

issue

1

eissn

0965-1748

issn

1879-0240

pii

S0965-1748(01)00081-9

journal_volume

32

pub_type

杂志文章

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