Abstract：
BACKGROUND/AIMS:The aim of this study was to investigate whether both matrix metalloproteinase-2 (MMP-2) and membrane type 1 MMP (MT1-MMP) participate in the spontaneous resolution of liver fibrosis. METHODS:Transcription of both genes was examined by reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization (ISH). Gelatinase activity was investigated by zymography. RESULTS:Gene expression by RT-PCR showed that both genes increased in the process of liver fibrosis, then decreased gradually in the recovery phase. ISH revealed that distribution of positive cells changed quickly in the recovery phase. Positive cells were widely seen in the liver, mainly around fibrous septa, in the aggressive phase, but were exclusively observed at the interface between the resolving fibrous band and the parenchyma, then were diffusely located in the lobules in the recovery phase. Main cells expressing both mRNAs seemed to be stellate cells for their morphology, though they did not express characteristic cell markers. Some hepatocytes and Kupffer cells expressed both mRNAs in the recovery phase. Gelatinase activity of MMP-2 increased in the recovery phase of 8-week-treated rat liver by gelatin zymography. CONCLUSIONS:The results of present study suggest that both enzymes participate in the destruction of extracellular matrix in coordination with MMP-13.
journal_name
J Hepatoljournal_title
Journal of hepatologyauthors
Watanabe T,Niioka M,Ishikawa A,Hozawa S,Arai M,Maruyama K,Okada A,Okazaki Idoi
10.1016/s0168-8278(01)00177-5subject
Has Abstractpub_date
2001-10-01 00:00:00pages
465-73issue
4eissn
0168-8278issn
1600-0641pii
S0168827801001775journal_volume
35pub_type
杂志文章abstract：BACKGROUND & AIMS:Autophagy plays a crucial role in hepatic homeostasis and its deregulation has been associated with chronic liver disease. However, the effect of autophagy on the release of fibrogenic extracellular vesicles (EVs) by platelet-derived growth factor (PDGF)-stimulated hepatic stellate cells (HSCs) remain...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2020.04.044
更新日期：2020-11-01 00:00:00
abstract：BACKGROUND & AIMS:Neonatal sclerosing cholangitis (NSC) is a severe neonatal-onset cholangiopathy commonly leading to liver transplantation (LT) for end-stage liver disease in childhood. Liver biopsy findings histopathologically resemble those in biliary atresia (BA); however, in NSC extrahepatic bile ducts are patent,...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2016.07.017
更新日期：2016-12-01 00:00:00
abstract：BACKGROUND & AIMS:Clinical cases of viral infections possibly involving the transfusion of blood components are systematically investigated. METHODS:Serological and molecular markers of hepatitis B virus were used including HBsAg, anti-HBc, anti-HBs, HBV DNA, and viral load. Full genome sequencing and phylogenetic ana...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2011.07.034
更新日期：2012-03-01 00:00:00
abstract：:Hepatitis B virus (HBV) variants with a stop codon, a mutated translation initiation codon or other mutations in the pre-C region which prevent e-antigen expression are highly prevalent in anti-HBe chronic carriers and can be positively selected from a mixed virus infection. Our laboratories recently described pre-C v...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(91)90083-n
更新日期：1991-11-01 00:00:00
abstract：BACKGROUND & AIMS:The therapeutic option for patients with chronic hepatitis delta virus infection (CHD) is limited to interferon alpha with rare curative outcome. Myrcludex B is a first-in-class entry inhibitor inactivating the hepatitis B virus (HBV) and hepatitis D virus (HDV) receptor sodium taurocholate co-transpo...
journal_title：Journal of hepatology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jhep.2016.04.016
更新日期：2016-09-01 00:00:00
abstract：BACKGROUND/AIMS:Chronic hepatitis C patients infected by genotype 1 are the least responsive to combination therapy and therefore monitoring response is important in identifying non-responders quickly, permitting therapy discontinuation and avoiding side effects and costs. We examined the usefulness of measuring total ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2003.11.026
更新日期：2004-03-01 00:00:00
abstract：BACKGROUND:Serum carbohydrate deficient transferrin is a marker of chronic alcohol consumption; it increases above normal in healthy individuals after a daily alcohol intake of more than 60 g/d for more than 2 weeks. The influence of liver disease itself on carbohydrate deficient transferrin levels has not been suffici...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(95)80037-9
更新日期：1995-12-01 00:00:00
abstract：:The coagulation inhibitor protein C was measured in 151 patients with various liver diseases. The protein C level was significantly decreased in patients with alcoholic cirrhosis (n = 73) compared to patients with steatosis (n = 24) (40 +/- 2%) vs. 88 +/- 4%, mean S.E., p less than 0.001). It was also decreased in cas...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(92)90153-g
更新日期：1992-03-01 00:00:00
abstract：BACKGROUND/AIMS:Alamifovir is a purine nucleotide analogue prodrug that shows potent activity against wild type and lamivudine resistant hepatitis B virus in preclinical studies. The aim of this study was to assess the safety and potential antiviral effects of alamifovir in humans. METHODS:A randomised, placebo contro...
journal_title：Journal of hepatology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.jhep.2004.07.028
更新日期：2004-11-01 00:00:00
abstract：:The multidrug resistance genes encode plasma membrane glycoproteins named P-glycoproteins, that act as an ATP-dependent drug efflux pump and decrease the cytosolic concentration of chemotherapeutic agents. It has been hypothesized that in rat liver, this protein may have a physiological role as a biliary transporter o...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(94)80235-1
更新日期：1994-11-01 00:00:00
abstract：:The EASL Monothematic Conference on Translational Research in Viral Hepatitis brought together a group of leading scientists and clinicians working on both, basic and clinical aspects of viral hepatitis, thereby building bridges from bench to bedside. This report recapitulates the presentations and discussions at the ...
journal_title：Journal of hepatology
pub_type：
doi：10.1016/j.jhep.2014.05.016
更新日期：2014-09-01 00:00:00
abstract：BACKGROUND:Platelet cytosolic calcium is necessary for normal platelet function, and may reflect intracellular signalling in vascular smooth muscle cells. METHODS:The cytosolic calcium of platelets from patients with cirrhosis and control subjects was measured in contact with plasma from either source, using FURA 2AM....
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(96)80117-6
更新日期：1996-09-01 00:00:00
abstract：:Suramin, a polybasic anion, blocks the activity of the DNA polymerase encoded by the duck hepatitis B virus. The interaction with the virus was studied under conditions in which the property of the drug to bind with proteins was used with plasma obtained from infected ducks with viraemia. Inhibition of DNA polymerase ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(85)80009-x
更新日期：1985-01-01 00:00:00
abstract：BACKGROUND & AIM:Natural killer T (NKT) cells are CD1d-restricted innate-like T cells that modulate innate and adaptive immune responses. Unlike the well-characterized invariant/type I NKT cells, type II NKT cells with a diverse T cell receptor repertoire are poorly understood. This study defines the pathogenic role of...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2017.05.024
更新日期：2017-10-01 00:00:00
abstract：:Human serum albumin (HSA) is one of the most frequent treatments in patients with decompensated cirrhosis. Prevention of paracentesis-induced circulatory dysfunction, prevention of type-1 HRS associated with bacterial infections, and treatment of type-1 hepatorenal syndrome are the main indications. In these indicatio...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2014.04.012
更新日期：2014-08-01 00:00:00
abstract：BACKGROUND/AIMS:Chronic graft hepatitis occurs in 20-30% adults after liver transplantation but the prevalence and causes in children are not known. In adults, hepatitis C virus infection is prevalent prior to transplantation and recurrent infection is a frequent cause of graft dysfunction. The significance of the rece...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(98)80225-0
更新日期：1998-05-01 00:00:00
abstract：BACKGROUND/AIM:Chronic rejection is an important cause of graft loss following liver transplantation. A number of risk factors for chronic rejection have been identified previously, albeit inconsistently. These include cytomegalovirus infection detected by a number of different techniques, including immunohistochemical...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(99)80294-3
更新日期：1999-11-01 00:00:00
abstract：:The peripheral arterial vasodilation hypothesis has been most influential in the field of cirrhosis and its complications. It has given rise to hundreds of pathophysiological studies in experimental and human cirrhosis and is the theoretical basis of life-saving treatments. It is undisputed that splanchnic arterial va...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2015.07.004
更新日期：2015-11-01 00:00:00
abstract：BACKGROUND/AIMS:Repetitive indomethacin administration induces down-regulation of hepatic cytochrome P450 (CYP) enzymes. We tested the hypothesis that an increase in intestinal permeability by indomethacin-induced intestinal injury leads to entry of bacterial endotoxin and reaching into liver via the portal vein, resul...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(03)00244-7
更新日期：2003-09-01 00:00:00
abstract：:Clinical complexity, anatomic diversity and molecular heterogeneity of cholangiocarcinoma (CCA) represent a major challenge in the assessment of effective targeted therapies. Molecular and cellular mechanisms underlying the diversity of CCA growth patterns remain a key issue of clinical concern. Crucial questions comp...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2014.09.007
更新日期：2015-01-01 00:00:00
abstract：:The addition of protease inhibitors, boceprevir or telaprevir, to peginterferon+ribavirin (PegIFN/RBV) increases the frequency as well as the severity, and hence, clinical relevance of anemia, which has now become one of the major complications associated with triple therapy. Most significant factors associated with a...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2013.07.014
更新日期：2013-12-01 00:00:00
abstract：BACKGROUND & AIMS:Weak hepatitis C virus (HCV) specific immunity in peripheral blood has been shown to be partially controlled by regulatory T cells (Treg). However, little is known about Treg present in livers of HCV-infected patients, their association with clinical parameters, and immunopathology resulting in diseas...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2009.12.013
更新日期：2010-03-01 00:00:00
abstract：BACKGROUND/AIMS:Immune mechanisms may modulate disease severity in chronic hepatitis C and the DR human leukocyte antigens may affect these mechanisms. Our aims were to evaluate the association between the DR antigens and disease severity at presentation and to seek correlation between these antigens, disease severity ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(96)80261-3
更新日期：1996-06-01 00:00:00
abstract：BACKGROUND/AIMS:Orthotopic liver transplantation has been shown to improve survival in fulminant hepatic failure. However, after orthotopic liver transplantation life-long immunosuppression is necessary and graft complications may occur. METHODS:We employed heterotopic auxiliary liver transplantation in a 26-year-old ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(95)80323-8
更新日期：1995-08-01 00:00:00
abstract：BACKGROUND/AIMS:The organic anion transporting polypeptides (OATPs) mediate the uptake of numerous amphipathic compounds into hepatocytes. Our aim was to study the expression and regulation of OATP8 (OATP1B3, SLC21A8/SLCO1B3) and OATP-C (OATP1B1, SLC21A6/SLCO1B1) in hepatocellular carcinomas (HCC). METHODS:RNA and pro...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2003.10.008
更新日期：2004-02-01 00:00:00
abstract：BACKGROUND & AIMS:HCV requires host lipid metabolism for replication, and apolipoproteins have been implicated in the response to treatment. METHODS:We examined plasma apolipoprotein concentrations in three cohorts of patients: mono-infected patients with symptomatic acute hepatitis C (aHCV); those undergoing treatmen...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2014.05.040
更新日期：2014-10-01 00:00:00
abstract：BACKGROUND & AIMS:Histopathology is the gold standard in the diagnosis of liver diseases but may be complicated by sampling error and bleeding. Confocal laser endomicroscopy (CLE) is a novel imaging modality providing in vivo histology. Previous studies using CLE for liver microscopy suffered from limited imaging depth...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2010.01.039
更新日期：2010-07-01 00:00:00
abstract：:The prolylhydroxylase inhibitor HOE 077 which has proven to be liver-selective in animals was tested in general pharmacological and toxicological studies for unwanted effects and safety. The results of these studies permit clinical development. A single-dose study in healthy volunteers was performed which showed that ...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/0168-8278(91)90009-z
更新日期：1991-01-01 00:00:00
abstract：:Twelve cirrhotic patients and six controls were fed an eucaloric high carbohydrate (CHO) diet for 3 days. Fasting serum triglyceride (TG), non-esterified fatty acids (NEFA), glucose, insulin and glycerol were estimated daily. On the 3rd day of the study we measured NEFA, glucose, insulin, and fructose every 45 min fro...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(92)90134-b
更新日期：1992-01-01 00:00:00
abstract：BACKGROUND/AIMS:Bacillus Calmette Guerin (BCG) infection causes hepatic injury following granuloma formation and secretion of cytokines which render mice highly sensitive to endotoxin-mediated hepatotoxicity. This work investigates the role of inducible nitric oxide synthase (iNOS) in liver damage induced by BCG and en...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2004.07.031
更新日期：2004-11-01 00:00:00
