Abstract:
:The development of multi-cellular organisms is regulated by the ordered definition of gene expression programmes that govern cell proliferation and differentiation. Although differential gene activity is mainly controlled by transcription factors, it is also dependent upon the underlying chromatin structure, which can stabilize transcriptional "on" or "off" states. We have recently isolated human (SUV39H1) and mouse (Suv39h1) histone methyltransferases (HMTases) and shown that they are important regulators for the organization of repressive chromatin domains. To investigate whether a SUV39H1-induced modulation of heterochromatin would affect mammalian development, we generated transgenic mice that over-express the SUV39H1 HMTase early during embryogenesis. SUV39H1 transgenic mice are growth retarded, display a weak penetrance of skeletal transformations and are largely characterized by impaired erythroid differentiation, consistent with highest transgene expression in foetal liver. Ex vivo transgenic foetal liver cultures initially contain reduced numbers of cells in G1 but progress to immortalized erythroblasts that are compromised in executing an erythroid differentiation programme. The outgrowing SUV39H1-immortalized erythroblasts can maintain a diploid karyotype despite deregulation of several tumour suppressor proteins and dispersed distribution of the heterochromatin component HP1. Together, these data provide evidence for a role of the SUV39H1 HMTase during the mammalian development and indicate a possible function for higher-order chromatin in contributing to the balance between proliferation and differentiation potentials of progenitor cells.
journal_name
Mech Devjournal_title
Mechanisms of developmentauthors
Czvitkovich S,Sauer S,Peters AH,Deiner E,Wolf A,Laible G,Opravil S,Beug H,Jenuwein Tdoi
10.1016/s0925-4773(01)00464-6subject
Has Abstractpub_date
2001-09-01 00:00:00pages
141-53issue
1-2eissn
0925-4773issn
1872-6356pii
S0925477301004646journal_volume
107pub_type
杂志文章abstract::In multicellular organisms, very little is known about the role of mRNA stability in development, and few proteins involved in degradation pathways have been characterized. We have identified the Drosophila homologue of XRN1, which is the major cytoplasmic 5'-3' exoribonuclease in Saccharomyces cerevisiae. The protein...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(98)00173-7
更新日期:1998-12-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(01)00664-5
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journal_title:Mechanisms of development
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更新日期:1999-06-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2001-02-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:2016-02-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(00)00356-7
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(91)90079-l
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(01)00363-x
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abstract::We have used whole mount in situ hybridization to analyze the pattern of expression of the gene Endo 16 in S. purpuratus embryos. The mRNA is first detectable at 18 h post-fertilization in the cytoplasm of blastomeres derived from the Veg2 6th cleavage tier. The number of Endo 16 positive cells increases gradually thr...
journal_title:Mechanisms of development
pub_type: 杂志文章
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更新日期:1993-08-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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abstract::The gene expression patterns of primordial germ cells (PGCs) and embryonic stem cells were analyzed by a modified serial analysis of gene expression. During the process, we cloned a novel gene, PGC7, which was preferentially expressed in PGCs. Immunohistochemical analysis revealed that PGC7 was specifically expressed ...
journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2004.12.011
更新日期:2005-05-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2004.01.008
更新日期:2004-03-01 00:00:00
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
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journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2010.12.002
更新日期:2011-03-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
pub_type: 杂志文章
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/0925-4773(92)90015-c
更新日期:1992-03-01 00:00:00
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journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/s0925-4773(00)00424-x
更新日期:2000-10-01 00:00:00
abstract::Early in the development of the imaginal wing disc of Drosophila, the LIM-HD gene tailup (islet), together with the HD genes of the iroquois complex, specify the notum territory of the disc. Later, tailup has been shown to act as a prepattern gene that antagonizes formation of sensory bristles on the notum of this fly...
journal_title:Mechanisms of development
pub_type: 杂志文章
doi:10.1016/j.mod.2010.05.001
更新日期:2010-09-01 00:00:00