Coexpression of endothelin-converting enzyme-1 and endothelin-1 in different stages of human atherosclerosis.


BACKGROUND:Endothelin-converting enzyme (ECE)-1 activates endothelin-1 (ET-1) and may thus contribute to the regulation of vascular tone and cell growth during atherosclerosis. METHODS AND RESULTS:To evaluate ECE-1 immunoreactivity concerning big ET-1/ET-1, we performed qualitative and quantitative immunohistochemistry in normal internal mammary arteries (n=10), in coronary arteries with adaptive intimal fibrosis (n=10), in aortic fatty streaks (n=10), and in distinct regions of advanced carotid plaques (n=15). Furthermore, we determined ECE-1 activity in the control specimens and in the inflammatory intimal regions of carotid plaques. Double immunolabeling showed that ECE-1 was present in endothelial cells, vascular smooth muscle cells, and macrophages. All ET-1(+) cells were simultaneously ECE-1(+). Most importantly, there were significantly more ET-1(+) cells in the intima and media when atherosclerosis was in an inflammatory stage than when it was in a noninflammatory stage. Moreover, ECE-1 activity was upregulated in the intima of carotid plaques, although immunohistochemically, there were no significant differences between the number of ECE(+) cells in the different compartments of the arterial wall. CONCLUSION:Together with ET-1, ECE-1 is abundantly present in human arteries and at different stages of atherosclerotic plaque evolution. The upregulation of the ECE-1/ET-1 system is closely linked to the presence of chronic inflammation and is present in very early stages of plaque evolution. Therefore, enhanced production of active ET-1 may substantially contribute to cell growth and the regulation of vascular tone in advanced atherosclerotic lesions and in the very early stages of plaque evolution, when a plaque is still imperceptible clinically.






Ihling C,Szombathy T,Bohrmann B,Brockhaus M,Schaefer HE,Loeffler BM




Has Abstract


2001-08-21 00:00:00












  • Coronary arterial spasm and Prinzmetal's variant form of angina induced by hyperventilation and Tris-buffer infusion.

    abstract::Vigorous hyperventilation was induced for five minutes immediately after a five-minute infusion of 100 ml of Tris-buffer (pH 10) in nine patients with Prinzmetal's variant angina. In eight of the patients, chest pain with ischemic changes in the electrocardiogram occurred during this procedure or within five minutes a...


    pub_type: 杂志文章


    authors: Yasue H,Nagao M,Omote S,Takizawa A,Miwa K,Tanaka S

    更新日期:1978-07-01 00:00:00

  • Linkage analysis and long QT syndrome. Using genetics to study cardiovascular disease.

    abstract:BACKGROUND:Recombinant DNA technologies have facilitated the development of a set of polymorphic DNA markers covering the human genome. General linkage analysis in families predisposed to inherited disease is now feasible. Linkage analysis can help identify a disease gene even when relatively little is known about the ...


    pub_type: 杂志文章,评审


    authors: Keating M

    更新日期:1992-06-01 00:00:00

  • Distribution of extracellular potassium and its relation to electrophysiologic changes during acute myocardial ischemia in the isolated perfused porcine heart.

    abstract::An experimental approach is described to quantitate inhomogeneity in extracellular K concentration ([K+]out) in the presence of ischemia and to relate this inhomogeneity to the electrophysiologic changes. Extracellular potassium concentration and local direct-current electrograms from the same sites were measured in i...


    pub_type: 杂志文章


    authors: Coronel R,Fiolet JW,Wilms-Schopman FJ,Schaapherder AF,Johnson TA,Gettes LS,Janse MJ

    更新日期:1988-05-01 00:00:00

  • Late reperfusion for acute myocardial infarction limits the dilatation of left ventricle without the reduction of infarct size.

    abstract:BACKGROUND:While previous clinical studies have shown a possible beneficial effect of the reperfusion performed at a relatively late phase of acute myocardial infarction ("late reperfusion") in preventing left ventricular enlargement, the mechanism has not been clarified. METHODS AND RESULTS:Of 89 patients with an ini...


    pub_type: 临床试验,杂志文章


    authors: Hirayama A,Adachi T,Asada S,Mishima M,Nanto S,Kusuoka H,Yamamoto K,Matsumura Y,Hori M,Inoue M

    更新日期:1993-12-01 00:00:00

  • Stimulated activation of platelet-derived growth factor receptor in vivo in balloon-injured arteries: a link between angiotensin II and intimal thickening.

    abstract:BACKGROUND:Growth factors such as platelet-derived growth factor (PDGF) have been postulated to be important mediators of neointimal formation in balloon-injured artery. Binding of growth factors to their receptors activates intrinsic receptor tyrosine kinase, resulting in tyrosine phosphorylation of receptors themselv...


    pub_type: 杂志文章


    authors: Abe J,Deguchi J,Matsumoto T,Takuwa N,Noda M,Ohno M,Makuuchi M,Kurokawa K,Takuwa Y

    更新日期:1997-09-16 00:00:00

  • Cardiovascular response to dynamic exercise in patients with chronic symptomatic mild-to-moderate and severe aortic regurgitation.

    abstract::Fifteen patients with symptomatic mild-to-moderate and severe chronic aortic regurgitation (AR) performed supine bicycle exercise while measurements of rest and exercise hemodynamics and left ventricular function were obtained. A continuous Doppler method was used to determine the change in distribution of total left ...


    pub_type: 杂志文章


    authors: Kawanishi DT,McKay CR,Chandraratna PA,Nanna M,Reid CL,Elkayam U,Siegel M,Rahimtoola SH

    更新日期:1986-01-01 00:00:00

  • G894T polymorphism in the endothelial nitric oxide synthase gene is associated with an enhanced vascular responsiveness to phenylephrine.

    abstract:BACKGROUND:Differences in vascular reactivity to phenylephrine (PE) responsiveness have been largely evidenced in patients undergoing cardiac surgery with cardiopulmonary bypass (CPB). Because nitric oxide (NO) strongly affects modulation of the vascular tone in response to vasopressor agents, we hypothesized that the ...


    pub_type: 临床试验,杂志文章


    authors: Philip I,Plantefeve G,Vuillaumier-Barrot S,Vicaut E,LeMarie C,Henrion D,Poirier O,Levy BI,Desmonts JM,Durand G,Benessiano J

    更新日期:1999-06-22 00:00:00

  • Tripling survival from sudden cardiac arrest via early defibrillation without traditional education in cardiopulmonary resuscitation.

    abstract:BACKGROUND:Early defibrillation is the most important intervention affecting survival from sudden cardiac arrest (SCA). To improve public access to early defibrillation, we established Piacenza Progetto Vita (PPV), the first system of out-of-hospital early defibrillation by first-responder volunteers. METHODS AND RESU...


    pub_type: 杂志文章


    authors: Capucci A,Aschieri D,Piepoli MF,Bardy GH,Iconomu E,Arvedi M

    更新日期:2002-08-27 00:00:00

  • Temporal Trends in Percutaneous Coronary Intervention Appropriateness: Insights From the Clinical Outcomes Assessment Program.

    abstract:BACKGROUND:It is unknown whether the appropriate use of percutaneous coronary intervention (PCI) has improved over time and whether trends in PCI appropriateness have been accompanied by changes in the use of PCI. METHODS AND RESULTS:We applied appropriate use criteria to determine the appropriateness of all 51 872 PC...


    pub_type: 杂志文章


    authors: Bradley SM,Bohn CM,Malenka DJ,Graham MM,Bryson CL,McCabe JM,Curtis JP,Lambert-Kerzner A,Maynard C

    更新日期:2015-07-07 00:00:00

  • Characterization of the cardiomyopathy in infants of diabetic mothers.

    abstract::A transient form of hypertrophic cardiomyopathy has been previously described in infants of diabetic mothers (IDMs). The purpose of this study was to determine the incidence, natural history and pathologic features of this cardiomyopathy in symptomatic and asymptomatic IDMs. We studied 47 IDMs for evidence of cardiomy...


    pub_type: 杂志文章


    authors: Gutgesell HP,Speer ME,Rosenberg HS

    更新日期:1980-02-01 00:00:00

  • Placental Growth Factor as an Indicator of Maternal Cardiovascular Risk After Pregnancy.

    abstract:BACKGROUND:Angiogenic placental growth factor (PlGF) concentrations rise during pregnancy, peaking at the end of midpregnancy. Low PlGF concentrations during pregnancy are associated with pregnancy complications with recognized later-life cardiovascular risk. We hypothesized that low PlGF concentrations, especially in ...


    pub_type: 杂志文章


    authors: Benschop L,Schalekamp-Timmermans S,Broere-Brown ZA,Roeters van Lennep JE,Jaddoe VWV,Roos-Hesselink JW,Ikram MK,Steegers EAP,Roberts JM,Gandley RE

    更新日期:2019-04-02 00:00:00

  • Incorporating a Genetic Risk Score Into Coronary Heart Disease Risk Estimates: Effect on Low-Density Lipoprotein Cholesterol Levels (the MI-GENES Clinical Trial).

    abstract:BACKGROUND:Whether knowledge of genetic risk for coronary heart disease (CHD) affects health-related outcomes is unknown. We investigated whether incorporating a genetic risk score (GRS) in CHD risk estimates lowers low-density lipoprotein cholesterol (LDL-C) levels. METHODS AND RESULTS:Participants (n=203, 45-65 year...


    pub_type: 杂志文章,随机对照试验


    authors: Kullo IJ,Jouni H,Austin EE,Brown SA,Kruisselbrink TM,Isseh IN,Haddad RA,Marroush TS,Shameer K,Olson JE,Broeckel U,Green RC,Schaid DJ,Montori VM,Bailey KR

    更新日期:2016-03-22 00:00:00

  • Tissue and serum angiogenic activity is associated with low prevalence of ischemic complications in patients with giant-cell arteritis.

    abstract:BACKGROUND:Vascular inflammatory lesions from patients with giant-cell arteritis show a remarkable amount of neovascularization, but its clinical implications have never been investigated. METHODS AND RESULTS:To assess the clinical relevance of neovascularization in giant-cell arteritis, angiogenesis was measured in t...


    pub_type: 临床试验,杂志文章


    authors: Cid MC,Hernández-Rodríguez J,Esteban MJ,Cebrián M,Gho YS,Font C,Urbano-Márquez A,Grau JM,Kleinman HK

    更新日期:2002-09-24 00:00:00

  • Randomized comparison of sirolimus-eluting stent versus standard stent for percutaneous coronary revascularization in diabetic patients: the diabetes and sirolimus-eluting stent (DIABETES) trial.

    abstract:BACKGROUND:Outcomes after percutaneous coronary interventions in diabetic patients are shadowed by the increased rate of recurrence compared with nondiabetic patients. METHODS AND RESULTS:We conducted a multicenter, randomized trial to demonstrate the efficacy of sirolimus-eluting stents compared with standard stents ...


    pub_type: 杂志文章,随机对照试验


    authors: Sabaté M,Jiménez-Quevedo P,Angiolillo DJ,Gómez-Hospital JA,Alfonso F,Hernández-Antolín R,Goicolea J,Bañuelos C,Escaned J,Moreno R,Fernández C,Fernández-Avilés F,Macaya C,DIABETES Investigators.

    更新日期:2005-10-04 00:00:00

  • Sex difference in risk of second but not of first venous thrombosis: paradox explained.

    abstract:BACKGROUND:The risk of recurrent venous thrombosis is 2-fold higher in men than in women. In contrast, no such sex difference in the risk of first venous thrombosis has been reported. We hypothesized that, for a first event, a risk difference between the sexes is masked by female exposure to reproductive factors (oral ...


    pub_type: 杂志文章


    authors: Roach RE,Lijfering WM,Rosendaal FR,Cannegieter SC,le Cessie S

    更新日期:2014-01-07 00:00:00

  • Patient selection and current practice strategy for off-pump coronary artery bypass surgery.

    abstract:OBJECTIVE:Previous studies comparing off-pump coronary artery bypass surgery (OPCABG) to conventional techniques utilizing cardiopulmonary bypass (CABG-CPB) have failed to provide patient selection guidelines. We sought to determine guidelines, attempting to rectify the limitations of previous studies. METHODS AND RES...


    pub_type: 杂志文章


    authors: Magee MJ,Coombs LP,Peterson ED,Mack MJ

    更新日期:2003-09-09 00:00:00

  • Absence of lethal reperfusion injury after 3 hours of reperfusion. A study in a single-canine-heart model of ischemia-reperfusion.

    abstract:BACKGROUND:Whether reperfusion can cause necrosis of previously viable myocytes (lethal reperfusion injury) remains controversial. Numerous studies examined the ability of various agents to prevent or limit reperfusion injury, but the results were contradictory. In a single-canine-heart model of ischemia-reperfusion, w...


    pub_type: 杂志文章


    authors: Zahger D,Yano J,Chaux A,Fishbein MC,Ganz W

    更新日期:1995-06-15 00:00:00

  • Temporal response and localization of integrins beta1 and beta3 in the heart after myocardial infarction: regulation by cytokines.

    abstract:BACKGROUND:Integrins are involved in structural remodeling and tissue repair. This study aimed to elucidate the role of the beta-integrins in cardiac remodeling after myocardial infarction (MI). METHODS AND RESULTS:The MI model was created by ligation of the left anterior descending coronary artery in rats. We detecte...


    pub_type: 杂志文章


    authors: Sun M,Opavsky MA,Stewart DJ,Rabinovitch M,Dawood F,Wen WH,Liu PP

    更新日期:2003-02-25 00:00:00

  • First postpacing interval variability during right ventricular stimulation: a single algorithm for the differential diagnosis of regular tachycardias.

    abstract:BACKGROUND:Failure to differentiate supraventricular from ventricular arrhythmias is the most frequent cause of inappropriate implantable cardioverter-defibrillator therapies. Although a sudden-onset criterion is available to differentiate sustained monomorphic ventricular tachycardias (SMVTs) and sinus tachycardias (S...


    pub_type: 杂志文章


    authors: Arenal A,Almendral J,Villacastin J,Morris R,Castellanos E,Delcan JL

    更新日期:1998-08-18 00:00:00

  • QT dispersion as an attribute of T-loop morphology.

    abstract:BACKGROUND:The suggestion that increased QT dispersion (QTD) is due to increased differences in local action potential durations within the myocardium is wanting. An alternative explanation was sought by relating QTD to vectorcardiographic T-loop morphology. METHODS AND RESULTS:The T loop is characterized by its ampli...


    pub_type: 杂志文章


    authors: Kors JA,van Herpen G,van Bemmel JH

    更新日期:1999-03-23 00:00:00

  • Vectorcardiographic criteria for the diagnosis of anterior myocardial infarction.

    abstract::Frank lead vectorcardiograms (VCG) from four carefully selected patient subgroups (226 patients) were analyzed to develop optimal criteria for the diagnosis of anterior myocardial infarction. Specificity was evaluated using 100 healthy volunteers under age 30 and 80 patients with normal left ventriculogram and normal ...


    pub_type: 杂志文章


    authors: Starr JW,Wagner GS,Draffin RM,Reed JB,Walston A 2nd,Behar VS

    更新日期:1976-02-01 00:00:00

  • Abnormal norepinephrine clearance and adrenergic receptor sensitivity in idiopathic orthostatic intolerance.

    abstract:BACKGROUND:Chronic orthostatic intolerance (OI) is characterized by symptoms of inadequate cerebral perfusion with standing, in the absence of significant orthostatic hypotension. A heart rate increase of >/=30 bpm is typical. Possible underlying pathophysiologies include hypovolemia, partial dysautonomia, or a primary...


    pub_type: 临床试验,杂志文章


    authors: Jacob G,Shannon JR,Costa F,Furlan R,Biaggioni I,Mosqueda-Garcia R,Robertson RM,Robertson D

    更新日期:1999-04-06 00:00:00

  • Effects of propranolol and nifedipine on exercise-induced attack in patients variant angina: assessment by exercise thallium-201 myocardial scintigraphy with quantitative rotational tomography.

    abstract::To examine the effects of propranolol and nifedipine on exercise-induced attack in patients with variant angina, exercise 201Tl myocardial scintigraphy with quantitative analysis by emission-computed tomography was performed in 20 patients with variant angina after oral propranolol (80 mg), nifedipine (20 mg), and pla...


    pub_type: 临床试验,杂志文章


    authors: Kugiyama K,Yasue H,Horio Y,Morikami Y,Fujii H,Koga Y,Kojima A,Takahashi M

    更新日期:1986-08-01 00:00:00

  • Five-Year Clinical Outcome and Valve Durability After Transcatheter Aortic Valve Replacement in High-Risk Patients.

    abstract:BACKGROUND:The FRANCE-2 registry (French Aortic National Corevalve and Edwards) previously reported good early- and medium-term clinical and echocardiographic efficacy for transcatheter aortic valve replacement. We here report 5-year follow-up results from the registry. METHODS:The registry includes all consecutive pa...


    pub_type: 杂志文章


    authors: Didier R,Eltchaninoff H,Donzeau-Gouge P,Chevreul K,Fajadet J,Leprince P,Leguerrier A,Lièvre M,Prat A,Teiger E,Lefevre T,Tchetché D,Carrié D,Himbert D,Albat B,Cribier A,Sudre A,Blanchard D,Rioufol G,Collet F,Houel

    更新日期:2018-12-04 00:00:00

  • Enhanced activity of variant phospholipase C-delta1 protein (R257H) detected in patients with coronary artery spasm.

    abstract:BACKGROUND:We recently demonstrated that phospholipase C (PLC)-delta1 activity in cultured skin fibroblasts obtained from patients with coronary spastic angina (CSA) is enhanced. We tested the hypothesis that structural abnormality in PLC-delta1 isoform is a cause of the enhanced activity. METHODS AND RESULTS:Sequence...


    pub_type: 杂志文章


    authors: Nakano T,Osanai T,Tomita H,Sekimata M,Homma Y,Okumura K

    更新日期:2002-04-30 00:00:00

  • Spatial heterogeneity of calcium transient alternans during the early phase of myocardial ischemia in the blood-perfused rabbit heart.

    abstract:BACKGROUND:Optical mapping of cytosolic calcium transients in intact mammalian hearts is now possible using long-wavelength [Ca(2+)](i) indicators. We propose that beat-to-beat [Ca(2+)](i) transient alternans during ischemia may lead to spatial and temporal heterogeneity of calcium-activated membrane currents. METHODS...


    pub_type: 杂志文章


    authors: Qian YW,Clusin WT,Lin SF,Han J,Sung RJ

    更新日期:2001-10-23 00:00:00

  • Effects of oxygen inhalation on skin microcirculation in patients with peripheral arterial occlusive disease.

    abstract:BACKGROUND:Oxygen administration is currently used in clinical medicine to improve peripheral oxygen delivery to tissues threatened by ischemia. However, conflicting results have been reported on the effects of oxygen in ischemic areas. This study was aimed at investigating the effects of 40% oxygen inhalation on the s...


    pub_type: 杂志文章


    authors: Bongard O,Bounameaux H,Fagrell B

    更新日期:1992-09-01 00:00:00

  • Cardiovascular responses to the isoprostanes iPF(2alpha)-III and iPE(2)-III are mediated via the thromboxane A(2) receptor in vivo.

    abstract:BACKGROUND:Isoprostanes (iPs) are free radical-catalyzed products of arachidonic acid that reflect lipid peroxidation in vivo. Several iPs exert biological effects in vitro and may contribute to the functional consequences of oxidant stress. For example, iPF(2alpha)-III (8-iso PGF(2alpha)) and iPE(2)-III modulate plate...


    pub_type: 杂志文章


    authors: Audoly LP,Rocca B,Fabre JE,Koller BH,Thomas D,Loeb AL,Coffman TM,FitzGerald GA

    更新日期:2000-06-20 00:00:00

  • Contribution of myocardium responsible for ventricular tachycardia to abnormalities detected by analysis of signal-averaged ECGs.

    abstract:BACKGROUND:Current methods of signal-averaged ECG analysis interrogate the terminal 40 msec of the QRS complex and/or the ST segment and have a low positive-predictive accuracy for detecting vulnerability to sustained ventricular tachycardia (VT). The extent to which abnormalities detected during these ECG intervals ar...


    pub_type: 杂志文章


    authors: Hood MA,Pogwizd SM,Peirick J,Cain ME

    更新日期:1992-12-01 00:00:00

  • Late dysrhythmias and disturbances of conduction following Mustard operation for complete transposition of the great arteries.

    abstract::Between 1964 and 1971, 49 patients were discharged from hospital following atrial baffle repair for transposition of the great arteries. Electrocardiograms of 47 of these patients were available for review seven months to eight and a half years (average 31 months) after operation. Of the 46 patients in sinus rhythm pr...


    pub_type: 杂志文章


    authors: Clarkson PM,Barratt-Boyles BG,Neutze JM

    更新日期:1976-03-01 00:00:00