Molecular cloning and characterization of a novel calcium/calmodulin-dependent protein kinase II inhibitor from human dendritic cells.

abstract：:Integral membrane lysophospholipid acyltransferases (AT) are involved in many reactions that produce phospholipids and triglycerides. Enzymes that utilize lysophosphatidic acid (LPA) as an acceptor substrate have been termed LPAATs, and several are members of the 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) ge...

journal_title：Biochemical and biophysical research communications

authors： Schmidt JA,Yvone GM,Brown WJ

更新日期：2010-07-09 00:00:00

abstract：:Recently, the concern that toluene might have carcinogenic and reproductive toxic potential has been raised. We investigated the ability of DNA damage by minor metabolites of toluene, methylhydroquinone, and methylbenzoquinone, using (32)P-5'-end-labeled DNA fragments obtained from the human genes. Methylhydroquinone ...

journal_title：Biochemical and biophysical research communications

authors： Murata M,Tsujikawa M,Kawanishi S

更新日期：1999-08-02 00:00:00

abstract：:We compared the nitric oxide (NO)-generating behavior of nitroglycerin (NTG), pentaerythritol trinitrate (PEtriN) and isosorbide dinitrate (ISDN), in the microsomal preparation of bovine coronary artery smooth muscle cells. The comparative NO generating activities among these nitrates were consistent with their relati...

journal_title：Biochemical and biophysical research communications

authors： Chung SJ,Fung HL

更新日期：1992-06-30 00:00:00

abstract：:Class-I phosphoinositide 3-kinases (PI 3-kinases) are dual specificity enzymes that possess both lipid and protein kinase activity. While the best characterized property of this protein kinase is as an autokinase activity, there have also been reports it can phosphorylate exogenous substrates including peptides, IRS-1...

journal_title：Biochemical and biophysical research communications

authors： Foukas LC,Shepherd PR

更新日期：2004-06-25 00:00:00

abstract：:Pierisin-2 is a cytotoxic and apoptosis-inducing protein present in Pieris brassicae with a 91% homology in the deduced amino acid sequences to pierisin-1 from Pieris rapae. We earlier showed pierisin-1 to catalyze mono(ADP-ribosyl)ation of 2'-deoxyguanosine (dG) in DNA to form N2-(ADP-ribos-1-yl)-2'-deoxyguanosine, t...

journal_title：Biochemical and biophysical research communications

authors： Takamura-Enya T,Watanabe M,Koyama K,Sugimura T,Wakabayashi K

更新日期：2004-10-15 00:00:00

abstract：:The ras family of oncogenes, as well as the transplasma membrane electron transport system, have been implicated in cellular growth control. Here we present evidence that the Ha-ras oncoprotein increases the activity of the transplasma membrane electron transport system in the mouse embryonic fibroblast cell line C3H1...

journal_title：Biochemical and biophysical research communications

authors： Crowe RA,Taparowsky EJ,Crane FL

更新日期：1993-10-29 00:00:00

abstract：:We previously demonstrated several nicotinic acetylcholine receptor (nAChR) subunits and associated proteins in human sperm. Here, we identified in sperm for the first time two additional nAChR-associated molecules: (1) agrin(SN)Z(+) in human sperm localized in the posterior post-acrosomal, neck, and flagellar mid-pie...

journal_title：Biochemical and biophysical research communications

authors： Kumar P,Ferns MJ,Meizel S

更新日期：2006-04-07 00:00:00

abstract：:Pre-treatment of neutrophils with either pertussis or cholera toxins does not inhibit neutrophil activation by surface bound IgG. In contrast, pretreatment with the phorbol ester, phorbol myristate acetate, results in a dose dependent inhibition of degranulation by surface bound IgG. This inhibition is similar to that...

journal_title：Biochemical and biophysical research communications

authors： Blackburn WD Jr,Heck LW

更新日期：1989-11-15 00:00:00

abstract：:In our previous studies, we have demonstrated that a stretch of amino-acid sequences identified from Arabidopsis ribosomal S6 kinase 1 (AtS6K1) provided a plant version of the TOS (TOR-signaling) motif, mediating the interaction with the Raptor protein in the TOR (Target of Rapamycin) kinase complex. Here we report th...

journal_title：Biochemical and biophysical research communications

authors： Son O,Kim S,Kim D,Hur YS,Kim J,Cheon CI

更新日期：2018-06-27 00:00:00

abstract：:Bcl10 is a signaling protein required for activation of the NF-kappaB transcription factor. NF-kappaB is an important mediator of genotoxic stress and regulates the expression of genes required for both cell proliferation and cell death. Bcl10 is phosphorylated in vivo, however, the protein kinase or kinases responsib...

journal_title：Biochemical and biophysical research communications

authors： Bodero AJ,Ye R,Lees-Miller SP

更新日期：2003-02-21 00:00:00

abstract：:We report here that at least seven low Mr GTP-binding proteins (range 21.5 to 29 kDa) are associated with the membranes of zymogen granules from rat pancreas. GTP binding proteins of similar Mr but in different relative proportions were found in the cytosolic fraction. Treatment of intact granules with either trypsin ...

journal_title：Biochemical and biophysical research communications

authors： Padfield PJ,Jamieson JD

更新日期：1991-01-31 00:00:00

abstract：:The effect of nicotinamide and flavin coenzymes on the 5-lipoxygenase activity has been determined in cell-free extracts from rat polymorphonuclear leukocytes. 5-lipoxygenase was assayed in the presence of 5-hydroperoxyeicosatetraenoic acid (5-HPETE), which caused a 3 to 4-fold stimulation in the maximal conversion of...

journal_title：Biochemical and biophysical research communications

authors： Riendeau D,Leblanc Y

更新日期：1986-12-15 00:00:00

abstract：:The α2-adrenoceptor inducer dexmedetomidine protects against acute lung injury (ALI), but the mechanism of this effect is largely unknown. The present study investigated the effect of dexmedetomidine on apoptosis induced by lipopolysaccharide (LPS) and the relationship between this effect and gap junction intercellula...

journal_title：Biochemical and biophysical research communications

authors： Zhang Y,Tan X,Xue L

更新日期：2018-01-01 00:00:00

abstract：:The conditions used in some immunoblot procedures can fail to detect calmodulin, S100 proteins, and other proteins with similar physical properties. We describe here some of the basis of this difficulty, and provide an immunoblot protocol that allows the rapid and reproducible detection of calmodulin and S100 beta in ...

journal_title：Biochemical and biophysical research communications

authors： Van Eldik LJ,Wolchok SR

更新日期：1984-11-14 00:00:00

abstract：:Effect of recombinant human interleukin-11 (rhIL-11) on the expression of transcripts encoding microsomal heme oxygenase (HO), the rate-limiting enzyme in heme catabolism, and haptoglobin (Hpt), a major acute-phase protein, were examined in human HepG2 hepatoma cells. Treatment of HepG2 cells with rhIL-11 elicited an ...

journal_title：Biochemical and biophysical research communications

authors： Fukuda Y,Sassa S

更新日期：1993-05-28 00:00:00

abstract：:The peroxidase and FAD-containing monooxygenase activities of porcine thyroid subcellular preparations were measured and it was observed that FAD-containing monooxygenase activity was considerably lower than that of peroxidase. The end product of 1-methyl-2[14C]thioimidazole oxidation catalysed by thyroid peroxidase w...

journal_title：Biochemical and biophysical research communications

authors： Paterson JR,Hood HT,Skellern GG

更新日期：1983-10-31 00:00:00

abstract：:Ghrelin, a novel growth-hormone-releasing acylated peptide, was recently isolated from rat stomach by the search of an endogenous ligand to an "orphan" G-protein-coupled-receptor. Ghrelin neuron is present in the arcuate nucleus of rat hypothalamus, but its central effect on growth hormone (GH) release has yet to be c...

journal_title：Biochemical and biophysical research communications

authors： Date Y,Murakami N,Kojima M,Kuroiwa T,Matsukura S,Kangawa K,Nakazato M

更新日期：2000-08-28 00:00:00

abstract：:Mammary epithelial cells derived from the entire mammary parenchyma or only end buds were isolated by collagenase digestion of mammary glands from virgin mice. Cells were cultured within collagen gels in serum-free medium containing insulin. Keratinocyte growth factor (KGF or FGF-7) and acidic fibroblast growth factor...

journal_title：Biochemical and biophysical research communications

authors： Imagawa W,Cunha GR,Young P,Nandi S

更新日期：1994-11-15 00:00:00

abstract：:Lysine-specific methyltransferase Set7/9 (KMT7) belongs to the SET domain family of proteins. Besides the SET domain, Set7/9 also contains a so-called MORN (Membrane Occupation and Recognition Nexus) domain whose function in high eukaryotes is largely unknown. Set7/9 has been shown to specifically methylate both histo...

journal_title：Biochemical and biophysical research communications

authors： Vasileva E,Shuvalov O,Petukhov A,Fedorova O,Daks A,Nader R,Barlev N

更新日期：2020-05-14 00:00:00

abstract：:Hyaluronan synthase 2 (HAS2) is an integral membrane protein composed of multi-membrane-spanning regions and a large intracellular loop (HAS2-loop). We previously examined the effect of phorbol 12-myristate 13-acetate (PMA) and/or 4-methylumbelliferone (4-MU) on the synthesis of hyaluronan (HA) in human skin fibroblas...

journal_title：Biochemical and biophysical research communications

authors： Kasai K,Kuroda Y,Takabuchi Y,Nitta A,Kobayashi T,Nozaka H,Miura T,Nakamura T

更新日期：2020-12-17 00:00:00

abstract：:Calphostin C (UCN-1028C), a newly isolated compound from Cladosporium cladosporioides, is a potent and specific inhibitor of protein kinase C, because it was 1000 times more inhibitory to protein kinase C (IC50, 0.05 microM) than other protein kinases such as cAMP-dependent protein kinase and tyrosine-specific protein...

journal_title：Biochemical and biophysical research communications

authors： Kobayashi E,Nakano H,Morimoto M,Tamaoki T

更新日期：1989-03-15 00:00:00

abstract：:Protein kinase C (PKC) is a family of serine/threonine kinases whose activity is controlled, in part, by phosphorylation on three conserved residues that are located on the catalytic domain of the enzyme, known as the activation-loop, the turn-motif, and the C-terminal hydrophobic-motif sites. Using a panel of phospho...

journal_title：Biochemical and biophysical research communications

authors： Freeley M,Volkov Y,Kelleher D,Long A

更新日期：2005-08-26 00:00:00

abstract：:ZinT (B1973), previously known as YodA, was originally characterised as a cadmium-induced periplasmic protein under the regulation of Fur and SoxS. Here we describe a decrease in zinT transcript in response to elevated copper concentrations and the zinc and copper dependent phenotype of a DeltazinT strain. Cadmium sen...

journal_title：Biochemical and biophysical research communications

authors： Kershaw CJ,Brown NL,Hobman JL

更新日期：2007-12-07 00:00:00

abstract：:The protein misfolding cyclic amplification (PMCA) assay allows for detection of prion protein misfolding activity in tissues and fluids from sheep with scrapie where it was previously undetected by conventional western blot and immunohistochemistry assays. Studies of goats with scrapie have yet to take advantage of P...

journal_title：Biochemical and biophysical research communications

authors： Madsen-Bouterse SA,Zhuang D,O'Rourke KI,Schneider DA

更新日期：2012-07-13 00:00:00

abstract：:Resonance energy transfer (RET) is widely used to detect proximity between biomolecules. In transparent solution the maximum donor-to-acceptor distance for RET is about 70 A. We measured the effects of metallic silver island films on RET from the intrinsic tryptophan of a protein to a bound probe as the acceptor. Thes...

journal_title：Biochemical and biophysical research communications

authors： Malicka J,Gryczynski I,Kusba J,Shen Y,Lakowicz JR

更新日期：2002-06-21 00:00:00

abstract：:The optimized geometry of the conformation of atoms constituting the 6-pyruvoyl tetrahydropterin molecule, the labile key intermediate of tetrahydrobiopterin biosynthesis, was obtained by molecular orbital calculations within the MINDO/3 framework. The stereostructure of the molecule showing the preferred mode for bin...

journal_title：Biochemical and biophysical research communications

authors： Katoh S,Sueoka T,Kurihara T

更新日期：1991-04-15 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma (PPARγ) participates in the process of insulin resistance (IR), a crucial pathophysiology in non-alcoholic fatty liver disease (NAFLD). Meanwhile, suppressor of cytokine signaling3 (SOCS3) also regulates IR in NAFLD. Both PPARγ and SOCS3 play a role in NAFLD through reg...

journal_title：Biochemical and biophysical research communications

authors： Bi J,Sun K,Wu H,Chen X,Tang H,Mao J

更新日期：2018-04-15 00:00:00

abstract：:Partial 3'-end nucleotide sequence of the pkI gene (GenBank accession No. AF019143) from Photobacterium leiognathi ATCC 25521 has been determined, and the encoded pyruvate kinase I is deduced. Pyruvate kinase I is the key enzyme of glycolysis, which converts phosphoenol pyruvate to pyruvate. Alignment and comparison o...

journal_title：Biochemical and biophysical research communications

authors： Lin JW,Lu HC,Chen HY,Weng SF

更新日期：1997-10-09 00:00:00

abstract：:Oxidized low-density lipoprotein (ox-LDL) is a critical mediator of atherogenesis. Macrophage uptake of ox-LDL and their subsequent development into foam cells is the principal event in atherosclerosis. Interleukin-1β (IL-1β), a prototypic multifunctional cytokine involved in inflammation, has an important effect on t...

journal_title：Biochemical and biophysical research communications

authors： Jiang Y,Wang M,Huang K,Zhang Z,Shao N,Zhang Y,Wang W,Wang S

更新日期：2012-08-24 00:00:00

abstract：:The factors which control the formation of lactiferous ducts during mammary gland development are not yet fully understood. To address this issue, we assessed the effect of the multifunctional cytokine transforming growth factor-beta-1 (TGF-beta1) on the morphogenetic properties of TAC-2.1 mammary epithelial cells gro...

journal_title：Biochemical and biophysical research communications

authors： Soriano JV,Orci L,Montesano R

更新日期：1996-03-27 00:00:00