Role of vitamin E in ascorbate-dependent protein thiol oxidation in rat liver endoplasmic reticulum.

abstract：:3-Methyl-substituted fatty acids are first oxidatively decarboxylated (alpha-oxidation) before they are degraded further via beta-oxidation. We synthesized [1-14C]phytanic and 3-[1-14C]methylmargaric acids in order to study their alpha-oxidation in isolated rat hepatocytes, rat liver homogenates and subcellular fracti...

journal_title：Archives of biochemistry and biophysics

authors： Huang S,Van Veldhoven PP,Vanhoutte F,Parmentier G,Eyssen HJ,Mannaerts GP

更新日期：1992-07-01 00:00:00

abstract：:Dipeptidyl-peptidase IV (EC 3.4.14.5) also known as CD26 is a membrane-bound serine peptidase which cleaves N-terminal dipeptides from a peptide chain containing a proline residue in the penultimate position. The enzyme is believed to play an important role in neuropeptide metabolism and T-cell activation. A series of...

journal_title：Archives of biochemistry and biophysics

authors： Li J,Wilk E,Wilk S

更新日期：1995-10-20 00:00:00

abstract：:The pH gradient, delta pH, present across the inner mitochondrial membrane in isolated rat hepatocytes was continuously monitored with a novel spectroscopic technique that utilizes the weak acid fluorescein. Unlike most cytosolic pH indicators, such as 2',7'-bis(carboxyethyl)-5,(6)-carboxyfluorescein (BCECF), fluoresc...

journal_title：Archives of biochemistry and biophysics

authors： Thomas PJ,Gaspers LD,Pharr C,Thomas JA

更新日期：1991-07-01 00:00:00

abstract：:We have successfully prepared E- and Z- isomers of 17-20 dehydro analogs of 2-methylene-19-nor-(20S)-1alpha,25-dihydroxyvitamin D3 (2MD). Both isomers bind to the recombinant rat vitamin D receptor (VDR) with high affinity. The Z-isomer (Vit-III 17-20Z) displays activity in vivo and in vitro that is similar to 2MD. Th...

journal_title：Archives of biochemistry and biophysics

authors： Vanhooke JL,Tadi BP,Benning MM,Plum LA,DeLuca HF

更新日期：2007-04-15 00:00:00

abstract：:Two enzymatically modified derivatives of tobramycin have been prepared by gentamicin nucleotidyl transferase-catalyzed adenylylation of tobramycin, using ATP and (Sp)-ATP alpha S as adenylylation substrates. (EC 2.7.7.46). The 1H, 13C, and 31P NMR spectra have been assigned for tobramycin, 2''-(adenosine-5'-phosphory...

journal_title：Archives of biochemistry and biophysics

authors： Van Pelt JE,Mooberry ES,Frey PA

更新日期：1990-08-01 00:00:00

abstract：:The effect of glutathione (GSH) on the superoxide-sensitive [4Fe-4S]-containing aconitase of Escherichia coli was explored. A mutant deficient in GSH biosynthesis, designated gshA, grew slower in a defined medium than did the parental strain and this effect was more pronounced when succinate was supplied as the carbon...

journal_title：Archives of biochemistry and biophysics

authors： Gardner PR,Fridovich I

更新日期：1993-02-15 00:00:00

abstract：:Previous studies have suggested that pyroptosis may play an important role in LPS-induced acute lung injury (ALI), but the exact mechanism of pyroptosis induction and the role of Angiotensin-converting enzyme 2 (ACE2)/Ang (1-7)/Mas axis in pyroptosis has not been investigated yet. The present study aimed to establish ...

journal_title：Archives of biochemistry and biophysics

authors： Huang H,Wang J,Liu Z,Gao F

更新日期：2020-10-30 00:00:00

abstract：:A cDNA that encodes the human plasminogen (HPg) amino acid sequence has been inserted adjacent to the polyhedrin promoter in the genome of the baculovirus, Autographa californica nuclear polyhedrosis virus, which was then used to infect cultured cells of the farm armyworm, Spodoptera frugiperda. Under the conditions o...

journal_title：Archives of biochemistry and biophysics

authors： Whitefleet-Smith J,Rosen E,McLinden J,Ploplis VA,Fraser MJ,Tomlinson JE,McLean JW,Castellino FJ

更新日期：1989-06-01 00:00:00

abstract：:The activity of thymidylate synthetase in the liver of the ddY strain male mouse increased transitorily according to the increase in tumor cell number at maximum 7-9 days after ip transplantation of Ehrlich ascites tumor. The enzyme was able to be purified from the tumor host mouse liver or from the normal mouse liver...

journal_title：Archives of biochemistry and biophysics

authors： Asanagi M,Moriyama Y,Fujimura S

更新日期：1988-12-01 00:00:00

abstract：:Subcutaneous implantation of demineralized bone matrix in rats induces migration of host cells into the site and results in the sequential development of cartilage and bone. The biosynthesis and metabolic fate of proteoglycans in the plaques at the bone matrix implantation site were investigated by [35S]sulfate labeli...

journal_title：Archives of biochemistry and biophysics

authors： Tian MY,Yanagishita M,Hascall VC,Reddi AH

更新日期：1986-05-15 00:00:00

abstract：:G protein-coupled receptors can be directly modulated by changes in transmembrane voltage in a variety of cell types. Here we show that, while changes in the membrane voltage itself do not induce detectable modifications in the cytosolic Ca(2+) concentration, platelet stimulation with thrombin or the PAR-1 and PAR-4 a...

journal_title：Archives of biochemistry and biophysics

authors： Albarrán L,Dionisio N,López E,Salido GM,Rosado JA

更新日期：2013-10-15 00:00:00

abstract：:An anti-avidin mAb, viz., H12G4, is shown to release bound biotin in a dose-dependent manner from holoavidin and holostreptavidin and inhibit the binding of ligand to the two apoproteins. The release of biotin by this mAb is accompanied by quenching of ligand-induced enhanced fluorescence of the FITC-avidin conjugate....

journal_title：Archives of biochemistry and biophysics

authors： Subramanian N,Subramanian S,Karande AA,Adiga PR

更新日期：1997-08-15 00:00:00

abstract：:Intrinsically disordered proteins represent about one third of eukaryotic proteins. An additional third correspond to proteins containing folded domains as well as large intrinsically disordered regions (IDR). While IDRs may represent functionally autonomous domains, in some instances it has become clear that they pro...

journal_title：Archives of biochemistry and biophysics

authors： Arbesú M,Pons M

更新日期：2019-11-30 00:00:00

abstract：:This work was purposed to speculate the possible association of rs2910164hsa-miR-146a C>G gene single nucleotide polymorphism in the pathogenesis of schizophrenia and subsequently their relevance to neuro-inflammatory, vascular and oxidative stress pathways as acute ischemic stroke (AIS) risk factors in chronic schizo...

journal_title：Archives of biochemistry and biophysics

authors： Ibrahim RR,Amer RA,Abozeid AA,Elsharaby RM,Shafik NM

更新日期：2020-01-15 00:00:00

abstract：:The zaragozic acids are potent inhibitors of squalene synthase. In vivo studies in mice confirmed our earlier observations that inhibition of squalene synthase by zaragozic acid A was accompanied by an increase in the incorporation of label from [3H]mevalonate into farnesyl-diphosphate (FPP)-derived isoprenoic acids (...

journal_title：Archives of biochemistry and biophysics

authors： Vaidya S,Bostedor R,Kurtz MM,Bergstrom JD,Bansal VS

更新日期：1998-07-01 00:00:00

abstract：:Cytosolic proteins may play an important role in the intracellular transport of bile acids in enterocytes. The lithocholate binding properties of cytosolic protein from bovine small intestine were studied. Lithocholate binding was observed in the Y (45-50 kDa), Y' (30-35 kDa), and Z fractions (10-15 kDa) following gel...

journal_title：Archives of biochemistry and biophysics

authors： Takikawa H,Arai S,Yamanaka M

更新日期：1992-11-01 00:00:00

abstract：:Reactive oxygen species (ROS) can be generated in experimental shock states through several different mechanisms. We measured ROS production in metabolically active liver mitochondria from rats rendered septic by cecal ligation and puncture. By polarography, the State 4 and State 3 respiration rates of liver mitochond...

journal_title：Archives of biochemistry and biophysics

authors： Taylor DE,Ghio AJ,Piantadosi CA

更新日期：1995-01-10 00:00:00

abstract：:Replication of the human immunodeficiency virus type 1 (HIV-1) is inhibited by interferons (IFNs), in part through activity of the IFN-inducible protein kinase PKR. To escape this antiviral effect, HIV-1 has developed strategies for blocking PKR function. We have previously shown that the HIV-1 Tat protein can associa...

journal_title：Archives of biochemistry and biophysics

authors： Cai R,Carpick B,Chun RF,Jeang KT,Williams BR

更新日期：2000-01-15 00:00:00

abstract：:After an energy source shift-down, Escherichia coli accumulates 70 S ribosome-mRNA complexes ("70 S monosomes"). The monosome mRNA strands are predominantly primary transcription products with purine nucleoside 5'-triphosphate and 5'-diphosphate termini present at a 1:2 ratio. The number-average chain length is 564 +/...

journal_title：Archives of biochemistry and biophysics

authors： Wnek AP,Jacobson LA

更新日期：1985-08-15 00:00:00

abstract：:The mitochondrial ATP synthase/ATPase (F0F1 ATPase) is perhaps the most complex enzyme known. In animal systems it consists of a minimum of 11 different polypeptide chains, 10 (or more) of which appear to be essential for function, and 1 called the "ATPase inhibitor peptide" which is involved in regulation. Recent stu...

journal_title：Archives of biochemistry and biophysics

authors： Schwerzmann K,Pedersen PL

更新日期：1986-10-01 00:00:00

abstract：:Swainsonine, a plant toxin, strongly inhibits certain alpha-D-mannosidases but has no effect on others [D. R. P. Tulsiani, T. M. Harris, and O. Touster (1982) J. Biol. Chem. 257, 7936-7939]. The reversible inhibition of jack bean and lysosomal alpha-D-mannosidases has previously been suggested to be similar in nature ...

journal_title：Archives of biochemistry and biophysics

authors： Tulsiani DR,Broquist HP,Touster O

更新日期：1985-01-01 00:00:00

abstract：:An S-adenosyl-L-methionine:caffeoyl-CoA 3-O-methyltransferase was purified 82-fold from elicitor-induced parsley cell suspension cultures by ammonium sulfate fractionation, anionic exchange and hydrophobic interaction chromatographies, and chromatofocusing. The enzyme has an apparent pI of 5.7 and a molecular weight o...

journal_title：Archives of biochemistry and biophysics

authors： Pakusch AE,Kneusel RE,Matern U

更新日期：1989-06-01 00:00:00

abstract：:The relative reactivities of lysine residues of tropomyosin complexed with troponin have been measured in order to locate the binding site of troponin on tropomyosin in a complex between the two native proteins. The lysines were labeled with acetic anhydride using a competitive labeling procedure and the relative reac...

journal_title：Archives of biochemistry and biophysics

authors： Hitchcock-DeGregori SE,Lewis SF,Mistrik M

更新日期：1988-08-01 00:00:00

abstract：:Developmentally regulated GTP-binding protein (DRG) is a new subfamily within the superfamily of GTP-binding proteins. Its expression is regulated during embryonic development. To investigate the effect of the expression of DRG2 on cell growth, we constructed a human Jurkat-T-cell line that overexpresses DRG2. Overexp...

journal_title：Archives of biochemistry and biophysics

authors： Ko MS,Lee UH,Kim SI,Kim HJ,Park JJ,Cha SJ,Kim SB,Song H,Chung DK,Han IS,Kwack K,Park JW

更新日期：2004-02-15 00:00:00

abstract：:Triosephosphate isomerase from rabbit has 5 Cys and 2 Met, while triosephosphate isomerase from yeast has 2 Cys (present in the rabbit enzyme in equivalent positions) and no Met. Since chloramine-T oxidizes Cys and Met, we determined the effect it has on the activity and structure of both enzymes. The activity of trio...

journal_title：Archives of biochemistry and biophysics

authors： Zubillaga RA,Pérez-Montfort R,Gómez-Puyou A

更新日期：1994-09-01 00:00:00

abstract：:The polymerization of scallop beta-like actin is significantly slower than that of skeletal muscle alpha-actin. To reveal which steps of polymerization contribute to this difference, we estimated the efficiency of nucleation of the two actins, the rates of filament elongation at spontaneous and gelsolin-nucleated poly...

journal_title：Archives of biochemistry and biophysics

authors： Khaitlina S,Hinssen H

更新日期：2008-09-15 00:00:00

abstract：:In vitro phosphorylation of purified spectrin dimer was studied in the presence of Ca2+-calmodulin (CaM). CaM inhibited autophosphorylation of the beta subunit of spectrin. The inhibitory effect (65% at a 32-fold molar excess) appeared to be due to a weak interaction of CaM with spectrin. CaM was similarly effective i...

journal_title：Archives of biochemistry and biophysics

authors： Maretzki D,Lutz HU

更新日期：1986-04-01 00:00:00

abstract：:Heme and iron are essential to almost all forms of life. The strict maintenance of heme and iron homeostasis is essential to prevent cellular toxicity and the existence of systemic and intracellular regulation is fundamental. Cytosolic heme can be catabolized and detoxified by heme oxygenases (HOs). Interestingly, fre...

journal_title：Archives of biochemistry and biophysics

authors： Yanatori I,Richardson DR,Toyokuni S,Kishi F

更新日期：2019-09-15 00:00:00

abstract：:Glutamine transport in both normal and acidotic rat kidney mitochondria was investigated using both isotopic techniques and by spectroscopic measurements in which glutamine metabolism was allowed to occur. Widely used criteria for demonstrating the occurrence of carrier-mediated transport were successfully applied in ...

journal_title：Archives of biochemistry and biophysics

authors： Atlante A,Passarella S,Minervini GM,Quagliariello E

更新日期：1994-12-01 00:00:00

abstract：:Pyruvate dehydrogenase (E1), an alpha(2)beta(2) tetramer, catalyzes the oxidative decarboxylation of pyruvate and reductive acetylation of lipoyl moieties of the dihydrolipoamide acetyltransferase. The roles of betaW135, alphaP188, alphaM181, alphaH15, and alphaR349 of E1 determined by kinetic analysis were reassessed...

journal_title：Archives of biochemistry and biophysics

authors： Korotchkina LG,Ciszak EM,Patel MS

更新日期：2004-09-15 00:00:00